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Detection of serum p53 antibodies from Chinese patients with papillary thyroid carcinoma using phage-SP-ELISA: correlation with clinical parameters

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Abstract

The goal of the present study was to investigate whether p53 antibodies (Abs) could be a relevant marker for papillary thyroid carcinoma (PTC). Three types of enzyme-linked immunosorbent assay (ELISA) methods were developed for the detection of p53 Abs, including p53-ELISA, phage-SS-ELISA, and phage-SP-ELISA. A total of 304 patients, including 117 cases with thyroid adenoma and 187 PTC patients, were enrolled in this study. Expression of p53 protein and mutation in BRAF gene were evaluated in paraffin-embedded tissue from 44 patients with PTC, in order to elucidate their correlations with the presence of p53 Abs. Compared with p53-ELISA and phage-SS-ELISA, phage-SP-ELISA presented the highest detection efficiency of p53 Abs in patients with PTC, and a combination of these three ELISA systems could make the detection of p53 Abs more sensitive than using each of the individual ELISA methods. Furthermore, p53 Abs was positively associated with clinical stage (P = 0.044), node metastasis (P = 0.010), and p53 protein accumulation (P = 0.019). These results indicate that serum p53 Abs could be a useful marker for PTC.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (Grant Number 81028010) and the Department of Science and Technology of Jilin Province, China (Grant Number 20130206009YY) and (Grant Number 20130727034YY).

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, 130024, China

    Pengtao Pan, Xiaoyi Han, Fangqian Li, Xiang Gao & Li Wang

  2. Jilin Provincial Key Laboratory of Surgical Translational Medicine, Division of Thyroid Surgery, China-Japan Union Hospital, Jilin University, Changchun, 130000, China

    Qingfeng Fu & Hui Sun

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  1. Pengtao Pan
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  2. Xiaoyi Han
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Correspondence to Hui Sun or Li Wang.

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Pan, P., Han, X., Li, F. et al. Detection of serum p53 antibodies from Chinese patients with papillary thyroid carcinoma using phage-SP-ELISA: correlation with clinical parameters. Endocrine 47, 543–549 (2014). https://doi.org/10.1007/s12020-014-0243-9

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  • DOI: https://doi.org/10.1007/s12020-014-0243-9

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