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The endothelial dysfunction in patients with type 2 diabetes mellitus is associated with IL-6 gene promoter polymorphism in Chinese population

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Abstract

The purpose of this study is to examine the effects of IL-6 gene promoter −174G/C and −572G/C polymorphism on endothelial function of Chinese T2DM and normal glucose regulation (NGR) subjects. 512 newly diagnosed T2DM patients and 483 NGR subjects were recruited and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was performed for the IL-6 gene promoter −174G/C and −572G/C polymorphism. Flow-mediated dilation (FMD) was measured as a non-invasive indicator for endothelial function. The results show that the C allele and CC genotype at −174 of IL-6 gene promoter region was extremely rare in both T2DM and NGR groups; genotypes’ and alleles’ frequency at −572 of IL-6 gene promoter region is of no difference between T2DM and NGR groups; within T2DM group, higher plasma IL-6 concentration and lower FMD was found in patients with −572 GC (2.36 ± 0.69, 4.23 ± 3.82%) or GG (2.32 ± 0.74, 4.24 ± 3.67%) genotype, compared with patients with CC (2.15 ± 0.62, 5.28 ± 3.94%) genotype. The conclusion of the study is that in comparison with patients of CC genotype, the T2DM patients of −572 GC or GG genotype may have more aggravated endothelial dysfunction (ED) and be at higher risk for coronary artery disease (CAD).

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Abbreviations

IL-6:

Interleukin-6

T2DM:

Type 2 diabetes mellitus

NGR:

Normal glucose regulation

CAD:

Coronary artery disease

ED:

Endothelial dysfunction

FMD:

Flow-mediated dilation

BMI:

Body mass index

SBP:

Systolic blood pressure

DBP:

Diastolic blood pressure

FPG:

Fasting plasma glucose

2 h PPG:

2 h Postloading plasma glucose

INS:

Insulin

HOMA-IR:

Homeostasis model assay of insulin resistance

TC:

Total cholesterol

TG:

Triglyceride

HDL-c:

High-density lipoprotein–cholesterol

LDL-c:

Low-density lipoproteincholesterol

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Acknowledgment

This work was supported by grant from Hangzhou Committee of Science and Technology (2005B012), we also thank all participants in the study.

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, Hangzhou Hospital, Nanjing Medical University, Xueshi Road 4#, Hangzhou City, Zhejiang Province, 310006, China

    Xianfeng Zhang, Lizhen Ma, Fengying Peng, Yin Wu, Yu Chen, Linying Yu, Zhikai Lei & Chu Zhang

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  1. Xianfeng Zhang
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  2. Lizhen Ma
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Correspondence to Chu Zhang.

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Zhang, X., Ma, L., Peng, F. et al. The endothelial dysfunction in patients with type 2 diabetes mellitus is associated with IL-6 gene promoter polymorphism in Chinese population. Endocrine 40, 124–129 (2011). https://doi.org/10.1007/s12020-011-9442-9

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  • DOI: https://doi.org/10.1007/s12020-011-9442-9

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