Skip to main content
SpringerLink
Log in

Analysis of angiotensin II- and ACTH-driven mineralocorticoid functions and omental adiposity in a non-genetic, hyperadipose female rat phenotype

  • Original Article
  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

The hypothalamic damage induced by neonatal treatment with monosodium l-glutamate (MSG) induces several metabolic abnormalities, resulting in a rat hyperleptinemic–hyperadipose phenotype. This study was conducted to explore the impact of the neonatal MSG treatment, in the adult (120 days old) female rat on: (a) the in vivo and in vitro mineralocorticoid responses to ACTH and angiotensin II (AII); (b) the effect of leptin on ACTH- and AII-stimulated mineralocorticoid secretions by isolated corticoadrenal cells; and (c) abdominal adiposity characteristics. Our data indicate that, compared with age-matched controls, MSG rats displayed: (1) enhanced and reduced mineralocorticoid responses to ACTH and AII treatments, respectively, effects observed in both in vivo and in vitro conditions; (2) adrenal refractoriness to the inhibitory effect of exogenous leptin on ACTH-stimulated aldosterone output by isolated adrenocortical cells; and (3) distorted omental adiposity morphology and function. This study supports that the adult hyperleptinemic MSG female rat is characterized by enhanced ACTH-driven mineralocorticoid function, impaired adrenal leptin sensitivity, and disrupted abdominal adiposity function. MSG rats could counteract undesirable effects of glucocorticoid excess, by developing a reduced AII-driven mineralocorticoid function. Thus, chronic hyperleptinemia could play a protective role against ACTH-mediated allostatic loads in the adrenal leptin resistant, MSG female rat phenotype.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. C.B. Nemeroff, L.D. Grant, G. Bissette et al., Psychoneuroendocrinology 2, 179–196 (1997)

    Article  Google Scholar 

  2. M.A. Holzwarth-McBride, J.R. Sladek Jr., K.M. Knigge, Anat. Rec. 186, 197–205 (1976)

    Article  CAS  PubMed  Google Scholar 

  3. D.T. Krieger, A.S. Liotta, G. Nicholsen, J.S. Kizer, Nature 278, 562–563 (1979)

    Article  CAS  PubMed  Google Scholar 

  4. J.W. Olney, L.G. Sharpe, Science 166, 386–388 (1969)

    Article  CAS  PubMed  Google Scholar 

  5. R.M. Burde, B. Schainker, J. Kayes, Nature 233, 58–60 (1971)

    Article  CAS  PubMed  Google Scholar 

  6. T.W. Redding, A.V. Schally, A. Arimura, I. Wakabayashi, Neuroendocrinology 8, 245–255 (1971)

    Article  CAS  PubMed  Google Scholar 

  7. P.J. Larsen, J.D. Mikkelsen, D. Jessop, S.L. Lightman, H.S. Chowdrey, J. Endocrinol. 141, 497–503 (1994)

    Article  CAS  PubMed  Google Scholar 

  8. I. Skultetyova, A. Kiss, D. Jezova, Neuroendocrinology 67, 412–420 (1998)

    Article  CAS  PubMed  Google Scholar 

  9. L. Macho, D. Jezova, S. Zorad, M. Fickova, Endocr. Regul. 33, 61–67 (1999)

    CAS  PubMed  Google Scholar 

  10. M.S. Dolnikoff, C.E. Kater, M. Egami, I.S. de Andrade, M.R. Marmo, Neuroendocrinology 48, 645–649 (1988)

    Article  CAS  PubMed  Google Scholar 

  11. M. Perelló, G. Moreno, G. Camihort et al., Endocrine 24, 167–175 (2004)

    Article  PubMed  Google Scholar 

  12. M. Perelló, G. Moreno, R.C. Gaillard, E. Spinedi, Neurendocrinol. Lett. 25, 119–126 (2004)

    Google Scholar 

  13. M. Perelló, R.C. Gaillard, A. Chisari, E. Spinedi, Neuroendocrinology 78, 176–184 (2003)

    Article  PubMed  Google Scholar 

  14. G. Moreno, M. Perelló, G. Camihort et al., Int. J. Obes. (Lond.) 30, 73–82 (2006)

    Article  CAS  Google Scholar 

  15. E. Spinedi, R.C. Gaillard, Endocrinology 139, 4016–4020 (1998)

    Article  CAS  PubMed  Google Scholar 

  16. F.P. Pralong, R. Roduit, G. Waeber et al., Endocrinology 139, 4264–4268 (1998)

    Article  CAS  PubMed  Google Scholar 

  17. M. Tena-Sempere, L. Pinilla, L.C. Gonzalez, F.F. Casanueva, C. Dieguez, E. Aguilar, J. Endocrinol. 167, 479–486 (2000)

    Article  CAS  PubMed  Google Scholar 

  18. M. Perello, D. Castrogiovanni, A. Giovambattista, R.C. Gaillard, E. Spinedi, Neuroendocrinology 89, 276–287 (2009)

    Article  CAS  PubMed  Google Scholar 

  19. N. Cherradi, A.M. Capponi, R.C. Gaillard, F.P. Pralong, Endocrinology 142, 3302–3308 (2001)

    Article  CAS  PubMed  Google Scholar 

  20. D. Tokarev, V. Kristova, M. Kriska, D. Jezova, Physiol. Res. 46, 165–171 (1997)

    CAS  PubMed  Google Scholar 

  21. B. Peters, S. Clausmeyer, N. Obermuller et al., J. Histochem. Cytochem. 46, 1215–1221 (1998)

    CAS  PubMed  Google Scholar 

  22. A. Glasow, A. Haidan, U. Hilbers et al., J. Clin. Endocrinol. Metab. 83, 4459–4466 (1998)

    Article  CAS  PubMed  Google Scholar 

  23. C. Salzmann, M. Otis, H. Long, C. Roberge, N. Gallo-Payet, C.D. Walker, Endocrinology 145, 1810–1822 (2004)

    Article  CAS  PubMed  Google Scholar 

  24. G.M. Reaven, H. Lithell, L. Landsberg, N. Engl. J. Med. 334, 374–381 (1996)

    Article  CAS  PubMed  Google Scholar 

  25. T.C. Friedman, G. Mastorakos, T.D. Newman et al., Endocr. J. 43, 645–655 (1996)

    Article  CAS  PubMed  Google Scholar 

  26. W.G. Haynes, W.I. Sivitz, D.A. Morgan, S.A. Walsh, A.L. Mark, Hypertension 30, 619–623 (1997)

    CAS  PubMed  Google Scholar 

  27. E.W. Shek, M.W. Brands, J.E. Hall, Hypertension 31, 409–414 (1998)

    CAS  PubMed  Google Scholar 

  28. M. Iwase, K. Ichikawa, K. Tashiro et al., Hypertens. Res. 23, 503–510 (2000)

    Article  CAS  PubMed  Google Scholar 

  29. F.A. Caputo, A.C. Scallet, Physiol. Behav. 58, 25–29 (1995)

    Article  CAS  PubMed  Google Scholar 

  30. L. Pinterova, B. Zelezna, M. Fickova et al., Horm. Metab. Res. 33, 708–712 (2001)

    Article  CAS  PubMed  Google Scholar 

  31. I. Rogulja, J.W. Harding, S. Ritter, Brain Res. 419, 333–335 (1987)

    Article  CAS  PubMed  Google Scholar 

  32. I. Kojima, K. Kojima, D. Kreutter, H. Rasmussen, J. Biol. Chem. 259, 14448–14457 (1984)

    CAS  PubMed  Google Scholar 

  33. R. Muzumdar, D.B. Allison, D.M. Huffman et al., Aging Cell. 7, 438–440 (2008)

    Article  CAS  PubMed  Google Scholar 

  34. C.C. Coimbra, R.H. Migliorini, Am. J. Physiol. 251, E703–E706 (1997)

    Google Scholar 

  35. M. Buyse, S. Viengchareun, A. Bado, M. Lombès, FASEB J. 15, 1357–1366 (2001)

    Article  CAS  PubMed  Google Scholar 

  36. E. Spinedi, C.A. Johnston, A. Negro-Vilar, Endocrinology 115, 267–272 (1984)

    Article  CAS  PubMed  Google Scholar 

  37. M. Kaneko, T. Hiroshige, J. Shinsako, M.F. Dallman, Am. J. Physiol. 39, R309–R316 (1980)

    Google Scholar 

  38. E. Spinedi, A. Negro-Vilar, Neuroendocrinology 37, 446–453 (1983)

    Article  CAS  PubMed  Google Scholar 

  39. E. Spinedi, L. Aguado, G. Basilotta, D. Carrizo, J. Endocrinol. Invest. 12, 321–327 (1989)

    CAS  PubMed  Google Scholar 

  40. G. Camihort, C. Gomez Dumm, G. Luna et al., Cells Tissues Organs 179, 192–201 (2005)

    Article  CAS  PubMed  Google Scholar 

  41. E. Spinedi, M. Giacomini, M.C. Jacquier, R.C. Gaillard, Neuroendocrinology 53, 160–170 (1991)

    Article  CAS  PubMed  Google Scholar 

  42. J. Piermaría, G. Console, M. Perelló, G. Moreno, R.C. Gaillard, E. Spinedi, Endocrine 20, 239–246 (2003)

    Article  PubMed  Google Scholar 

  43. E. Spinedi, A. Negro-Vilar, Endocrinology 114, 2247–2251 (1984)

    Article  CAS  PubMed  Google Scholar 

  44. W.D. McElroy, C.P. Swanson (eds.), Biostatistical Analysis (Prentice-Hall-Englewood Cliffs, New Jersey, 1974)

    Google Scholar 

Download references

Acknowledgments

Authors are indebted to Mr. O Vercellini and Mrs. C Ferese for animal care and histological preparations, respectively. The editorial assistance of Mrs. G. Vigo is recognized. This work was supported by grants from FONCyT (PICT 1051-2007) and CONICET (PIP 0704), Argentina, and FPRE (07-09), Switzerland.

Author information

Authors and Affiliations

  1. Neuroendocrine Unit, Multidisciplinary Institute on Cell Biology (CONICET-CICPBA), PO Box 403, 1900, La Plata, Argentina

    Mario Perelló & Eduardo Spinedi

  2. Department of Histology and Embryology “B”, School of Medicine, UNLP-CICPBA, 1900, La Plata, Argentina

    Gloria Cónsole

  3. Division of Endocrinology, Diabetology and Metabolism, University Hospital (CHUV), 1011, Lausanne, Switzerland

    Rolf C. Gaillard

Authors
  1. Mario Perelló
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gloria Cónsole
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rolf C. Gaillard
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eduardo Spinedi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eduardo Spinedi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perelló, M., Cónsole, G., Gaillard, R.C. et al. Analysis of angiotensin II- and ACTH-driven mineralocorticoid functions and omental adiposity in a non-genetic, hyperadipose female rat phenotype. Endocr 37, 497–506 (2010). https://doi.org/10.1007/s12020-010-9335-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12020-010-9335-3

Keywords

Search

Navigation