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Epithelial progenitor 1, a novel factor associated with epithelial cell growth and differentiation

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Abstract

The growth and renewal of epithelial tissue is a highly orchestrated and tightly regulated process occurring in different tissue types under a variety of circumstances. We have been studying the process of pancreatic regeneration in mice. We have identified a cell surface protein, named EP1, which is expressed on the duct epithelium during pancreatic regeneration. Whereas it is not detected in the pancreas of normal mice, it is found in the intestinal epithelium of normal adult mice, as well as during pancreatic repair following cerulein-induced destruction of the acinar tissue. The distinctive situations in which EP1 is expressed, all of which share in common epithelial cell growth in the gastrointestinal tract, suggest that EP1 is involved in the growth and renewal of epithelial tissues in both the intestine and the pancreas.

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Abbreviations

NOD:

Non-obese diabetic

SCID:

Severe combined immunodeficiency

FACS:

Fluorescence activated cell sorting

LCA:

Leukocyte common antigen

SDF1a:

Stromal-derived-factor-1 alpha

CXCR4:

Chemokine (C-X-C motif) receptor 4

CCK:

Cholecystokinin

panCK:

Pancytokeratin

BrdU:

Bromodeoxyuridine

Ly-6:

Lymphocyte antigen 6

uPAR:

Urokinase-type plasminogen activator receptor

LU:

Ly-6 antigen/uPA receptor-like domain

PLA2:

Phospholipase A2

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Acknowledgments

We would like to thank Zhong Chen for his excellent technical contributions to this study. We would like to thank the members of the Sarvetnick lab for their helpful comments during the preparation of this article. We would like to thank Dr. Jason Cyster, UCSF, for giving us the SDF1a transgenic mice. This study was supported by grant DK 60746, awarded to Nora Sarvetnick by the National Institute of Health. MRK was funded by an Advanced Postdoctoral Fellowship from the JDRF; SAD was funded by an ACSBI fellowship of the International Union Against Cancer (UICC); YQZ was supported by a fellowship from NIH training grant T32 HL00795 and a career development award from the Crohn’s and Colitis Foundation of America and HH was supported by a fellowship from the Larry L. Hillblom Foundation. This manuscript number 18816 from The Scripps Research Institute.

Author information

Author notes

  1. Cory U. Lago

    Present address: Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA

  2. Ayse G. Kayali

    Present address: The Islet Research Laboratory, Whittier Institute for Diabetes, Department of Pediatrics, School of Medicine, University of California San Diego, 9894 Genesee Ave, La Jolla, CA, 92037, USA

  3. Sandrine Arnaud-Dabernat

    Present address: Laboratoire de la Biologie de la Differenciation et du Developpement, University Victor Segalen, Bordeaux 2, 146 rue Leo Saignat, 33076, Bordeaux cedex, France

  4. Guoxun Liu

    Present address: Genomic Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Dr, San Diego, CA, 92121, USA

  5. Hong Hua

    Present address: Johnson and Johnson PRDS, Internal Medicine, 3210 Merryfield Row, San Diego, CA, 92121, USA

Authors and Affiliations

  1. Department of Immunology, The Scripps Research Institute, La Jolla, CA, 92037, USA

    Marcie R. Kritzik, Cory U. Lago, Ayse G. Kayali, Sandrine Arnaud-Dabernat, Guoxun Liu, You-Qing Zhang, Hong Hua & Nora E. Sarvetnick

  2. Molecular and Integrative Neuroscience Department, The Scripps Research Institute, La Jolla, CA, 92037, USA

    Howard S. Fox

  3. Department of Pharmacology and Experimental Neuroscience, Leid Transplant Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    Howard S. Fox

  4. Department of Surgery, Leid Transplant Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    Marcie R. Kritzik & Nora E. Sarvetnick

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  1. Marcie R. Kritzik
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Correspondence to Nora E. Sarvetnick.

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Kritzik, M.R., Lago, C.U., Kayali, A.G. et al. Epithelial progenitor 1, a novel factor associated with epithelial cell growth and differentiation. Endocr 37, 312–321 (2010). https://doi.org/10.1007/s12020-009-9297-5

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  • DOI: https://doi.org/10.1007/s12020-009-9297-5

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