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Progesterone and estradiol effects on SRC-1 and SRC-3 expression in human astrocytoma cell lines

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Abstract

Progesterone (P4) and estradiol (E2) regulate many cell functions through their interaction with specific intracellular receptors, which require the participation of coactivators such as SRC-1 and SRC-3 for enhancing their transcriptional activity. Coactivator expression is altered in many cancers and in some of them their expression is regulated by P4 and E2. In this study, we determined progesterone and estrogen receptor isoform expression in two human astrocytoma cell lines with different evolution grade (U373, grade III; and D54, grade IV) by Western Blot. We studied the role of P4 and E2 on SRC-1 and SRC-3 expression in U373 and D54 cell lines by RT–PCR and Western blot. In U373 cells, P4 did not modify SRC-1 expression, but in D54 cells it increased SRC-1 mRNA expression after 12 h of treatment without significant changes after 24 h. P4 also increased SRC-1 protein content after 24 h, but reduced it after 48 h. E2 did not change SRC-1 expression in any cell line. SRC-3 expression was not regulated by either E2 or P4. Our data suggest that SRC-1 and SRC-3 expression is differentially regulated by sex steroid hormones in astrocytomas and that P4 regulates SRC-1 expression depending on the evolution grade of human astrocytoma cells.

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Acknowledgments

This study was supported by Consejo Nacional de Ciencia y Tecnología (project No. 100645), México.

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Authors and Affiliations

  1. Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Coyoacán, Mexico D.F, Mexico

    Olivia Tania Hernández-Hernández, Aliesha González-Arenas & Ignacio Camacho-Arroyo

  2. Instituto Nacional de Medicina Genómica, Mexico City, Mexico D.F, Mexico

    Mauricio Rodríguez-Dorantes

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  1. Olivia Tania Hernández-Hernández
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  2. Mauricio Rodríguez-Dorantes
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  3. Aliesha González-Arenas
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  4. Ignacio Camacho-Arroyo
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Correspondence to Ignacio Camacho-Arroyo.

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Hernández-Hernández, O.T., Rodríguez-Dorantes, M., González-Arenas, A. et al. Progesterone and estradiol effects on SRC-1 and SRC-3 expression in human astrocytoma cell lines. Endocr 37, 194–200 (2010). https://doi.org/10.1007/s12020-009-9288-6

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  • DOI: https://doi.org/10.1007/s12020-009-9288-6

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