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Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry

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Abstract

Orexin neuropeptides participate in the regulation of feeding as well as the regulation and maintenance of wakefulness and the cognitive functions. Orexin A and B share a common precursor, prepro-orexin and neurons are localized in the lateral hypothalamus. Physiological studies showed that these neurons are regulated by glutamatergic innervations. We aimed to assess the effects of kainic acid as a potent agonist for non-NMDA glutamate receptors in the activation of orexin neurons. We also analyzed the effect of glutamate antagonist CNQX, injected prior to kainic acid, on this activation. Expression of c-Fos protein was used as a marker for neuronal activation. Dual immunohistochemical labeling was performed for prepro-orexin and c-Fos and the percentages of c-Fos-expressing orexin neurons were obtained for control, kainic acid, and CNQX groups. Kainic acid injection caused statistically significant increase in the number of c-Fos-positive neurons when compared to control group (62.69 and 36.31%, respectively). Activation of orexin neurons was blocked, in part, by CNQX (43.36%). In the light of these results, it is concluded that glutamate takes part in the regulation of orexin neurons and partially exerts its effects through non-NMDA glutamate receptors and that orexin neurons express functional non-NMDA receptors.

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Acknowledgments

This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) through a “Career” Grant (104S286-SBAG-K-74 to OE).

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Authors and Affiliations

  1. Department of Histology and Embryology, Uludag University Faculty of Medicine, Gorukle Kampusu, 16059, Bursa, Turkey

    Ozhan Eyigor, Zehra Minbay & Ilkin Cavusoglu

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  1. Ozhan Eyigor
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  2. Zehra Minbay
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  3. Ilkin Cavusoglu
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Correspondence to Ozhan Eyigor.

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Eyigor, O., Minbay, Z. & Cavusoglu, I. Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry. Endocr 37, 167–172 (2010). https://doi.org/10.1007/s12020-009-9284-x

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  • DOI: https://doi.org/10.1007/s12020-009-9284-x

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