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Age, body mass index, and serum level of DHEA-S can predict glucocorticoid receptor function in women with polycystic ovary syndrome

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Abstract

Glucocorticoid receptor (GR) transduces the glucocorticoid (GC) signal that could lead to metabolic derangements depending on the tissue responsiveness to GC. We aimed to investigate possible causative relation of the GR functional properties in peripheral blood mononuclear cells of women with polycystic ovary syndrome (PCOS), with their clinical and biochemical characteristics. Thirty women with PCOS [mean age: 26.5 ± 5.1 years, mean body mass index (BMI) 24.5 ± 5 kg/m2], and thirty respective controls were analyzed for the number of GR sites per cell (B max), apparent equilibrium dissociation constant (K d), and binding potency (GR potency). A strong association between B max and K d (r = 0.70, P < 0.0001), and GR potency with age (r = 0.49, P = 0.009) was observed in PCOS women. The multiple regression analyses within the PCOS group revealed that independent predictors for K d were BMI, total cholesterol, and dehydroepiandrosterone-sulfate (DHEA-S) (r = 0.58, P = 0.038), while for GR potency (r = 0.687, P = 0.013) were age, BMI, DHEA-S, and basal cortisol concentration. The results suggest that PCOS pathophysiology may be related to alterations of a cross stalk between glucocorticoid signaling, age, and metabolic parameters. These findings should be further explored in studies on the role of GR in PCOS-related metabolic derangements.

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Acknowledgments

This work was supported by the Ministry of Science of Serbia (Grant number 143003).

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Authors and Affiliations

  1. Institute of Endocrinology, Diabetes and Metabolic Diseases, Clinical Center of Serbia, Dr Subotića 13, 11000, Belgrade, Serbia

    Djuro Macut, Ivana Božić, Milan Petakov & Svetozar Damjanović

  2. Department of Biochemistry, Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia

    Danijela Vojnović Milutinović, Gordana Matić & Jelena Brkljačić

  3. Division of Endocrinology and Human Reproduction, 2nd Department of Obstetrics and Gynecology, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Dimitrios Panidis & Nikolaos Spanos

  4. Department of Endocrinology, CHC Bežanijska kosa, Belgrade, Serbia

    Jelica Bjekić

  5. Institute of Physiology, School of Medicine, University of Belgrade, Belgrade, Serbia

    Olivera Stanojlović

  6. Institute for Student’s Health, University of Belgrade, Belgrade, Serbia

    Anđela Petrović Milinković

  7. Faculty of Organizational Sciences, Institute for Statistics, University of Belgrade, Belgrade, Serbia

    Zoran Radojičić

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  1. Djuro Macut
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  2. Danijela Vojnović Milutinović
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  13. Svetozar Damjanović
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Correspondence to Djuro Macut.

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Macut, D., Vojnović Milutinović, D., Božić, I. et al. Age, body mass index, and serum level of DHEA-S can predict glucocorticoid receptor function in women with polycystic ovary syndrome. Endocr 37, 129–134 (2010). https://doi.org/10.1007/s12020-009-9277-9

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  • DOI: https://doi.org/10.1007/s12020-009-9277-9

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