Skip to main content

STEAP1 is over-expressed in breast cancer and down-regulated by 17β-estradiol in MCF-7 cells and in the rat mammary gland

Endocrine volume 34, pages 108–116 (2008)Cite this article

Abstract

Six transmembrane epithelial antigen of the prostate 1 (STEAP1) was identified as a prostate-specific cell-surface antigen over-expressed in prostate cancer, and in human cancer cell lines obtained from several other tissues. Its cell surface location in all tumor types analyzed so far, and its absence in most vital organs in humans, turned STEAP1 into a potential target for anti-tumor immunotherapy. This study provides experimental evidence that STEAP1 is also over-expressed in human breast cancer cases, and in normal breast tissue adjacent to breast tumors, where it is localized in the cell membrane of epithelial cells. It is also demonstrated that STEAP1 transcription correlates negatively with estrogen receptor (ER) immunoreactivity, and positively with tumor grading in breast cancer cases. As estrogens are involved in breast cancer onset and progression, the response of STEAP1 to 17β-estradiol (E2) was investigated in the mammary gland of rats, and in the human breast cancer cell line, MCF-7. These experiments demonstrated that STEAP1 is down-regulated by E2 in both models. The mechanisms underlying the STEAP1 response to E2 in vitro were further investigated in MCF-7 cells, and the results obtained suggest an effect mediated by the membrane-bound ERα (mbERα).

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. R.S. Hubert, I. Vivanco, E. Chen, S. Rastegar, K. Leong, S.C. Mitchell, R. Madraswala, Y. Zhou, J. Kuo, A.B. Raitano, A. Jakobovits, D.C. Saffran, D.E. Afar, Proc. Natl. Acad. Sci. USA 96, 14523–14528 (1999)

    CAS  Article  PubMed  Google Scholar 

  2. D. Yang, G.E. Holt, M.P. Velders, E.D. Kwon, W.M. Kast, Cancer Res. 61, 5857–5860 (2001)

    CAS  PubMed  Google Scholar 

  3. P.M. Challita-Eid, K. Morrison, S. Etessami, Z. An, K.J. Morrison, J.J. Perez-Villar, A.B. Raitano, X.C. Jia, J.M. Gudas, S.B. Kanner, A. Jakobovits, Cancer Res. 67, 5798–5805 (2007)

    CAS  Article  PubMed  Google Scholar 

  4. H. Kobayashi, T. Nagato, K. Sato, N. Aoki, S. Kimura, M. Murakami, H. Iizuka, M. Azumi, H. Kakizaki, M. Tateno, E. Celis, Cancer Res. 67, 5498–5504 (2007)

    CAS  Article  PubMed  Google Scholar 

  5. A. Machlenkin, A. Paz, E. Bar Haim, O. Goldberger, E. Finkel, B. Tirosh, I. Volovitz, E. Vadai, G. Lugassy, S. Cytron, F. Lemonnier, E. Tzehoval, L. Eisenbach, Cancer Res. 65, 6435–6442 (2005)

    CAS  Article  PubMed  Google Scholar 

  6. D.A. Rodeberg, R.A. Nuss, S.F. Elsawa, E. Celis, Clin. Cancer Res. 11, 4545–4552 (2005)

    CAS  Article  PubMed  Google Scholar 

  7. P.M. Alves, O. Faure, S. Graff-Dubois, S. Cornet, I. Bolonakis, D.A. Gross, I. Miconnet, S. Chouaib, K. Fizazi, J.C. Soria, F.A. Lemonnier, K. Kosmatopoulos, Cancer Immunol. Immunother. 55, 1515–1523 (2006)

    CAS  Article  PubMed  Google Scholar 

  8. L. Garcia-Hernandez Mde, A. Gray, B. Hubby, W.M. Kast, Cancer Res. 67, 1344–1351 (2007)

    Article  PubMed  Google Scholar 

  9. M. Iscan, T. Klaavuniemi, T. Coban, N. Kapucuoglu, O. Pelkonen, H. Raunio, Breast Cancer Res. Treat. 70, 47–54 (2001)

    CAS  Article  PubMed  Google Scholar 

  10. Z. Huang, M.J. Fasco, H.L. Figge, K. Keyomarsi, L.S. Kaminsky, Drug Metab. Dispos. 24, 899–905 (1996)

    CAS  PubMed  Google Scholar 

  11. H. Hellmold, T. Rylander, M. Magnusson, E. Reihner, M. Warner, J.A. Gustafsson, J. Clin. Endocrinol. Metab. 83, 886–895 (1998)

    CAS  Article  PubMed  Google Scholar 

  12. C.M. Yballe, T.H. Vu, A.R. Hoffman, J. Clin. Endocrinol. Metab. 81, 1607–1612 (1996)

    CAS  Article  PubMed  Google Scholar 

  13. Z.P. Pavelic, L. Pavelic, E.E. Lower, M. Gapany, S. Gapany, E.A. Barker, H.D. Preisler, Cancer Res. 52, 2597–2602 (1992)

    CAS  PubMed  Google Scholar 

  14. G. Deng, L.C. Chen, D.R. Schott, A. Thor, V. Bhargava, B.M. Ljung, K. Chew, H.S. Smith, Cancer Res. 54, 499–505 (1994)

    CAS  PubMed  Google Scholar 

  15. F. Forozan, E.H. Mahlamaki, O. Monni, Y. Chen, R. Veldman, Y. Jiang, G.C. Gooden, S.P. Ethier, A. Kallioniemi, O.P. Kallioniemi, Cancer Res. 60, 4519–4525 (2000)

    CAS  PubMed  Google Scholar 

  16. F. Forozan, R. Veldman, C.A. Ammerman, N.Z. Parsa, A. Kallioniemi, O.P. Kallioniemi, S.P. Ethier, Br. J. Cancer 81, 1328–1334 (1999)

    CAS  Article  PubMed  Google Scholar 

  17. R. Jakesz, Int. J. Gynecol. Cancer 16(Suppl 2), 511–514 (2006)

    Article  PubMed  Google Scholar 

  18. P. Monje, S. Zanello, M. Holick, R. Boland, Mol. Cell. Endocrinol. 181, 117–129 (2001)

    CAS  Article  PubMed  Google Scholar 

  19. C.E. Powell, A.M. Soto, C. Sonnenschein, J. Steroid Biochem. Mol. Biol. 77, 97–108 (2001)

    CAS  Article  PubMed  Google Scholar 

  20. D. Zivadinovic, B. Gametchu, C.S. Watson, Breast Cancer Res. 7, R101–R112 (2005)

    CAS  Article  PubMed  Google Scholar 

  21. D. Zivadinovic, C.S. Watson, Breast Cancer Res. 7, R130–R144 (2005)

    CAS  Article  PubMed  Google Scholar 

  22. C.W. Elston, I.O. Ellis, Histopathology 19, 403–410 (1991)

    CAS  Article  PubMed  Google Scholar 

  23. M.W. Pfaffl, Nucleic Acids Res. 29, e45 (2001)

    CAS  Article  PubMed  Google Scholar 

  24. F.C. Schmitt, M.J. Bento, I. Amendoeira, Diagn. Cytopathol. 13, 347–351 (1995)

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgments

Maia CJB is recipient of a fellowship from the Portuguese Foundation for Science and Technology-FCT, SFRH/BD/13388/2003. All the experimental work was supported by the FCT plurianual program. We acknowledge Dr. Eugenio Serrano de Dios from the University Hospital of Salamanca and Centro Hospitalar da Cova da Beira for providing breast cancer samples, Catarina Ferreira for the preparation of tissue sections, and Dr. Graça Baltazar and Telma Quintela for counselling in fluorescence microscopy.

Author information

Affiliations

  1. Centre of Investigation in Health Sciences—CICS, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal

    Cláudio J. B. Maia, Sílvia Socorro & Cecília R. A. Santos

  2. IPATIMUP—Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal

    Fernando Schmitt

  3. Medical Faculty of Porto University, Porto, Portugal

    Fernando Schmitt

Authors
  1. Cláudio J. B. Maia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sílvia Socorro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fernando Schmitt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cecília R. A. Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cecília R. A. Santos.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Maia, C.J.B., Socorro, S., Schmitt, F. et al. STEAP1 is over-expressed in breast cancer and down-regulated by 17β-estradiol in MCF-7 cells and in the rat mammary gland. Endocr 34, 108–116 (2008). https://doi.org/10.1007/s12020-008-9113-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12020-008-9113-7

Keywords

  • Breast cancer
  • 17β-estradiol
  • Gene expression
  • Immunohistochemistry
  • Real-time PCR
  • STEAP1