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Fat storage is partially dependent on vagal activity and insulin secretion of hypothalamic obese rat

Endocrine volume 31, pages 142–148 (2007)Cite this article

Abstract

Hypothalamic MSG-obese rats show hyperinsulinemia and tissue insulin resistance, and they display intense parasympathetic activity. Current analysis investigates whether early subdiaphragmatic vagotomy prevents tissue insulin sensitivity impairment in adult obese MSG-rats. Hypothalamic obesity was induced by MSG (4 mg/g BW), daily, from birth up to 5 days. Control animals receiving saline solution. On the 30th day rats underwent bilateral subdiaphragmatic vagotomy or sham surgery. An intravenous glucose tolerance test (ivGTT) was performed when rats turned 90 days old. Total white fat tissue (WAT) from rat carcass was extracted and isolated; the interscapular brown fat tissue (IBAT) was weighed. Rather than blocking obesity, vagotomy reduced WAT and IBAT in MSG-obese rats when the latter were compared to sham MSG-rats. High blood fasting insulin and normal glucose levels were also observed in MSG-obese rats. Although glucose intolerance, high insulin secretion, and significant insulin resistance were recorded, vagotomy improved fasting insulinemia, glucose tolerance and insulin tissue sensitivity in MSG-obese rats. Results suggest that increased fat accumulation is caused, at least in part, by high blood insulin concentration, and enhanced parasympathetic activity on MSG-obese rats.

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Acknowledgment

This work was supported by Brazilian Research Agencies CNPq and CAPES.

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Authors and Affiliations

  1. Laboratory of Secretion Cell Biology, Department of Cell Biology and Genetics, State University of Maringá, Avenida Colombo 5790, Maringa, PR, 87020-900, Brazil

    Sandra Lucinei Balbo, Sabrina Grassiolli, Rosane Aparecida Ribeiro, Clarice Gravena, Ana Eliza Andreazzi & Paulo Cezar de Freitas Mathias

  2. Department of Physiology, State University of Western Paraná, Cascavel, PR, 85814-110, Brazil

    Maria Lúcia Bonfleur

  3. Department of Physiology, State University of Maringá, Maringa, PR, 87020-900, Brazil

    Marcia do Nascimento Brito & Rosana Torrezan

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  1. Sandra Lucinei Balbo
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Correspondence to Paulo Cezar de Freitas Mathias.

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Balbo, S.L., Grassiolli, S., Ribeiro, R.A. et al. Fat storage is partially dependent on vagal activity and insulin secretion of hypothalamic obese rat. Endocr 31, 142–148 (2007). https://doi.org/10.1007/s12020-007-0021-z

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  • DOI: https://doi.org/10.1007/s12020-007-0021-z

Keywords

  • MSG-obesity
  • Parasympathetic activity
  • Vagotomy
  • Insulin resistance
  • Fat tissue