Abstract
The clathrin assembly protein, CALM, promotes the assembly of clathrin-coated vesicles. In a previous study, we showed that CALM controls the level of the synaptic vesicle protein VAMP2 at the plasma membrane by regulating VAMP2 endocytosis. Here, we provide evidence that CALM also influences the cell surface level of the AMPA receptor subunit GluR2. Although mechanistic details as well as the physiological relevance of CALM and GluR2 in the neuron have yet to be established, CALM-mediated trafficking could function as a component of a dedicated system for controlling postsynaptic abundance of GluR2.
Similar content being viewed by others
References
De Felice, F. G., Velasco, P. T., Lambert, M. P., Viola, K., Fernandez, S. J., Ferreira, S. T., et al. (2007). Abeta oligomers induce neuronal oxidative stress through an N-methyl-d-aspartate receptor-dependent mechanism that is blocked by the Alzheimer drug memantine. Journal of Biological Chemistry, 282, 11590–11601.
Dreyling, M. H., Martinez-Climent, J. A., Zheng, M., Mao, J., Rowley, J. D., & Bohlander, S. K. (1996). The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family. Proceedings of the National Academy of Science, 93, 4804–4809.
Harel, A., Wu, F., Mattson, M. P., Morris, C. M., & Yao, P. J. (2008). Evidence for CALM in directing VAMP2 trafficking. Traffic, 9, 417–429.
Harold, D., Abraham, R., Hollingworth, P., Sims, R., Gerrish, A., Hamshere, M. L., et al. (2009). Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer’s disease. Nature Genetics, 10, 1088–1093.
Hsieh, H., Boehm, J., Sato, C., Iwatsubo, T., Tomita, T., Sisodia, S., et al. (2006). AMPAR removal underlies Abeta-induced synaptic depression and dendritic spine loss. Neuron, 52, 831–843.
Lisman, J., & Raghavachari, S. (2006). A unified model of the presynaptic and postsynaptic changes during LTP at CA1 synapses. Science STKE, 2006(356), re11.
McCann, C. M., Bareyre, F. M., Lichtman, J. W., & Sanes, J. R. (2005). Peptide tags for labeling membrane proteins in live cells with multiple fluorophores. Biotechniques, 38, 945–952.
Newpher, T. M., & Ehlers, M. D. (2008). Glutamate receptor dynamics in dendritic microdomains. Neuron, 58, 472–497.
Opazo, P., & Choquet, D. (2010). A three-step model for the synaptic recruitment of AMPA receptors. Molecular Cellular Neurosciences [Epub ahead of print].
Plant, K., Pelkey, K. A., Bortolotto, Z. A., Morita, D., Terashima, A., McBain, C. J., et al. (2006). Transient incorporation of native GluR2-lacking AMPA receptors during hippocampal long-term potentiation. Nature Neuroscience, 9, 593–594.
Schmid, E. M., & McMahon, H. T. (2007). Integrating molecular and network biology to decode endocytosis. Nature, 448, 883–888.
Schwartz, C. M., Cheng, A., Mughal, M. R., Mattson, M. P., & Yao, P. J. (2010). Clathrin assembly proteins AP180 and CALM in the embryonic rat brain. Journal of Comparative Neurology, 518, 3803–3818.
Sekine-Aizawa, Y., & Huganir, R. L. (2004). Imaging of receptor trafficking by using α- bungarotoxin-binding-tagged receptors. Proceedings of the National Academy of Science, 101, 17114–17119.
Tebar, F., Bohlander, S. K., & Sorkin, A. (1999). Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic. Molecular Biology of the Cell, 10, 2687–2702.
Tokuoka, H., & Goda, Y. (2008). Activity-dependent coordination of presynaptic release probability and postsynaptic GluR2 abundance at single synapses. Proceedings of the National Academy of Science, 105, 14656–14661.
Traub, L. M. (2009). Tickets to ride: selecting cargo for clathrin-regulated internalization. Nature Reviews Molecular Cell Biology, 10, 583–596.
Ungewickell, E. J., & Hinrichsen, L. (2007). Endocytosis: clathrin-mediated membrane budding. Current Opinion in Cell Biology, 19, 417–425.
Yao, P. J., Petralia, R. S., Bushlin, I., Wang, Y., & Furukawa, K. (2005). Synaptic distribution of the endocytic accessory proteins AP180 and CALM. Journal of Comparative Neurology, 481, 58–69.
Acknowledgments
This work was supported by the Intramural Research Program of the National Institute on Aging of the NIH.
Author information
Authors and Affiliations
Corresponding author
Additional information
Addendum to:
Harel A, Wu F, Mattson MP, Morris CM, Yao PJ. Evidence for CALM in directing VAMP2 trafficking. Traffic 2008; 9:417-429.
Rights and permissions
About this article
Cite this article
Harel, A., Mattson, M.P. & Yao, P.J. CALM, A Clathrin Assembly Protein, Influences Cell Surface GluR2 Abundance. Neuromol Med 13, 88–90 (2011). https://doi.org/10.1007/s12017-010-8142-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12017-010-8142-6