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Anti-α-Actinin Antibodies Cross-react with Anti-ssDNA Antibodies in Active Autoimmune Hepatitis

Abstract

Recently, we have observed that the association of anti-F-actin autoantibodies (Abs) with anti-α-actinin Abs (double reactivity) was correlated with liver disease activity in autoimmune hepatitis type 1 (AIH-1; Guéguen et al., J Clin Immunol 26:495–505, 2006). As anti-α-actinin Abs are also associated with anti-single-stranded (ss) DNA in 16 of 50 (32%) AIH-1 patients, compared with 16 of 401 (4.0%) liver disease control patients, we sought to characterize these Abs in AIH-1. When associated, anti-ssDNA Abs cross-react with anti-α-actinin Abs, but neither with anti-double-stranded DNA Abs nor with anti-F-actin Abs. Furthermore, anti-α-actinin Abs are associated with high-avidity anti-ssDNA Abs in AIH-1. In addition, double reactivity against α-actinin and ssDNA was correlated with clinical and histological activity of the disease and untreated AIH-1 patients.

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References

  1. 1.

    Zachou K, Rigopoulou E, Dalekos GN (2004) Autoantibodies and autoantigens in autoimmune hepatitis: important tools in clinical practice and to study pathogenesis of the disease. J Autoimmun Dis 1:2

    Article  Google Scholar 

  2. 2.

    Guéguen P, Dalekos G, Nousbaum JB et al (2006) Double reactivity against actin and alpha-actinin defines a severe form of autoimmune hepatitis type 1. J Clin Immunol 26:495–505

    PubMed  Article  Google Scholar 

  3. 3.

    Mostoslavsky G, Fischel R, Yachimovich N et al (2001) Lupus anti-DNA autoantibodies cross-react with a glomerular structural protein: a case for tissue injury by molecular mimicry. Eur J Immunol 31:1221–1227

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Deocharan B, Qing X, Lichauco J, Putterman C (2002) Alpha-actinin is a cross-reactive renal target for pathogenic anti-DNA antibodies. J Immunol 168:3072–3078

    PubMed  CAS  Google Scholar 

  5. 5.

    Alvarez F, Berg PA, Bianchi FB et al (1999) International autoimmune hepatitis group report: review of criteria for diagnosis of autoimmune hepatitis. J Hepatol 31:929–938

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Croquefer S, Renaudineau Y, Jousse S et al (2005) The anti-alpha-actinin test completes anti-DNA determination in systemic lupus erythematosus. Ann NY Acad Sci 1050:170–175

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    Renaudineau Y, Croquefer S, Jousse S et al (2006) Association of alpha-actinin-binding anti-double-stranded DNA antibodies with lupus nephritis. Arthritis Rheum 54:2523–2532

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Mason LJ, Ravirajan CT, Rahman A, Putterman C, Isenberg DA (2004) Is alpha-actinin a target for pathogenic anti-DNA antibodies in lupus nephritis? Arthritis Rheum 50:866–870

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Leibovitch L, George J, Levi Y, Bakimer R, Shoenfeld Y (1995) Anti-actin antibodies in sera from patients with autoimmune liver diseases and patients with carcinomas by ELISA. Immunol Lett 48:129–132

    PubMed  Article  CAS  Google Scholar 

  10. 10.

    Dalekos GN, Makri E, Loges S et al (2002) Increased incidence of anti-LKM autoantibodies in a consecutive cohort of hepatitis C patients from central Greece. Eur J Gastroenterol Hepatol 14:35–42

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    Dalekos GN, Wedemeyer H, Obermayer-straub P et al (1999) Epitope mapping of cytochrome P4502D6 autoantigen in patients with chronic hepatitis C during alpha-interferon treatment. J Hepatol 30:366–375

    PubMed  Article  CAS  Google Scholar 

  12. 12.

    Renaudineau Y, Revelen R, Bordron A, Mottier D, Youinou P, Le corre R (1998) Two populations of endothelial cell antibodies cross-react with heparin. Lupus 7:86–94

    PubMed  Article  CAS  Google Scholar 

  13. 13.

    Moritoki Y, Lian ZX, Ohsugi Y, Ueno Y, Gershwin ME (2006) B cells and autoimmune liver diseases. Autoimmun Rev 5:449–457

    PubMed  Article  CAS  Google Scholar 

  14. 14.

    Youinou P (2007) B cell conducts the lymphocyte orchestra. J Autoimmun 28:143–151

    PubMed  Article  CAS  Google Scholar 

  15. 15.

    Christen U, Holdener M, Hintermann E (2007) Animal models for autoimmune hepatitis. Autoimmun Rev 6:306–311

    PubMed  Article  CAS  Google Scholar 

  16. 16.

    Renaudineau Y, Deocharan B, Jousse S, Renaudineau E, Putterman C, Youinou P (2007) Anti-alpha-actinin antibodies: a new marker of lupus nephritis. Autoimmun Rev 6:464–468

    PubMed  Article  CAS  Google Scholar 

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Acknowledgments

Many thanks are due to Cindy Séné for secretarial assistance. This work has been supported in part by a grant (#2466) of the Research Committee, University of Thessaly.

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Correspondence to Pierre Youinou.

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Renaudineau, Y., Dalekos, G.N., Guéguen, P. et al. Anti-α-Actinin Antibodies Cross-react with Anti-ssDNA Antibodies in Active Autoimmune Hepatitis. Clinic Rev Allerg Immunol 34, 321–325 (2008). https://doi.org/10.1007/s12016-007-8050-1

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Keywords

  • Autoimmune hepatitis type 1
  • Anti-DNA
  • Anti-α-actinin