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Sorting Technology for Mesenchymal Stem Cells from a Single Tissue Source

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Abstract

Mesenchymal stem cells (MSCs) are adult stem cells that can be obtained, enriched and proliferated in vitro. They owned enormous potential in fields like regenerative medicine, tissue engineering and immunomodulation. However, though isolated from the same origin, MSCs are still essentially heterogeneous cell populations with different phenotypes and functions. This heterogeneity of MSCs significantly affects their therapeutic efficacy and brings obstacles to scientific research. Thus, reliable sorting technology which can isolate or purify MSC subpopulations with various potential and differentiation pathways is urgently needed. This review summarized principles, application status and clinical implications for these sorting methods, aiming at improving the understanding of MSC heterogeneity as well as providing fresh perspectives for subsequent clinical applications.

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Funding

This study was funded by the National Natural Science Foundation of China (Grant No.81772104, No.81701929, No.81971889, No.81902013, No.82003085, No.82202468), the Natural Science Foundation of Guangdong Province (Grant No.2017A030310120, Grant No.2019A1515012170). The Foundation of Guangzhou Municipal Science and Technology Bureau (SL2022A04J02027). Nanfang Hospital Distinguished Young cultivation program (2022J003).

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  1. Xinyi Feng and Fangfang Qi contributed equally to this study.

Authors and Affiliations

  1. Department of Plastic and Aesthetic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, China

    Fangfang Qi, Hailin Wang, Wenzhen Li, Yuyang Gan, Zhen Lin, Lu Chen, Piao Wang, Zhiqi Hu & Yong Miao

  2. The First Clinical School of Southern Medical University, Guangzhou, China

    Xinyi Feng & Caiyu Qi

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  1. Xinyi Feng
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Contributions

Xinyi Feng: Original draft preparation, Manuscript revision. Fangfang Qi: Literature review and Editing, Manuscript revision. Hailin Wang: Review-specifically critical review. Wenzhen Li: Manuscript revision. Yuyang Gan: Review-commentary. Caiyu Qi: Editing-format layout. Zhen Lin: Review and revise manuscripts. Lu Chen: Visualization-data presentation. Piao Wang: Development of methodology. Zhiqi Hu: Oversight and leadership responsibility for the group. Yong Miao: Supervisor.

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Correspondence to Zhiqi Hu or Yong Miao.

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Feng, X., Qi, F., Wang, H. et al. Sorting Technology for Mesenchymal Stem Cells from a Single Tissue Source. Stem Cell Rev and Rep 20, 524–537 (2024). https://doi.org/10.1007/s12015-023-10635-w

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