![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12015-023-10634-x/MediaObjects/12015_2023_10634_Fig1_HTML.png)
Data Availability (data transparency)
The data that support the findings of this study are available from the corresponding author, upon request.
Code Availability (software application or custom code)
Not applicable.
References
Mengatto, C. M., Mussano, F., Honda, Y., Colwell, C. S., & Nishimura, I. (2011). Circadian rhythm and cartilage extracellular matrix genes in osseointegration: A genome-wide screening of implant failure by vitamin D deficiency. PLoS One, 6(1), e15848.
Karsenty, G. (2001). Central control of bone formation. Advances in Nephrology from the Necker Hospital, 31, 119–133.
Hassan, N., McCarville, K., Morinaga, K., Mengatto, C. M., Langfelder, P., Hokugo, A., Tahara, Y., Colwell, C. S., & Nishimura, I. (2017). Titanium biomaterials with complex surfaces induced aberrant peripheral circadian rhythms in bone marrow mesenchymal stromal cells. PLoS One, 12(8), e0183359.
Morinaga, K., Sasaki, H., Park, S., Hokugo, A., Okawa, H., Tahara, Y., Colwell, C. S., & Nishimura, I. (2019). Neuronal PAS domain 2 (Npas2) facilitated osseointegration of titanium implant with rough surface through a neuroskeletal mechanism. Biomaterials, 192, 62–74.
Lin, T. H., Tang, C. H., Hung, S. Y., Liu, S. H., Lin, Y. M., Fu, W. M., & Yang, R. S. (2010). Upregulation of heme oxygenase-1 inhibits the maturation and mineralization of osteoblasts. Journal of Cellular Physiology, 222(3), 757–768.
Goldstein, L., Teng, Z. P., Zeserson, E., Patel, M., & Regan, R. F. (2003). Hemin induces an iron-dependent, oxidative injury to human neuron-like cells. Journal of Neuroscience Research, 73(1), 113–121.
International Standards Organization (2009). ANSI/AAMI/ ISO 10993-5: 2009/(R)2014. Part 5: Tests for in vitro cytotoxicity. In: The Association for the Advancement of Medical Instrumentation. Biological evaluation of medical devices Arlington, VA.
West, A. R., & Oates, P. S. (2008). Subcellular location of heme oxygenase 1 and 2 and divalent metal transporter 1 in relation to endocytotic markers during heme iron absorption. Journal of Gastroenterology and Hepatology, 23(1), 150–158.
Khatiwala, C. B., Peyton, S. R., & Putnam, A. J. (2006). Intrinsic mechanical properties of the extracellular matrix affect the behavior of pre-osteoblastic MC3T3-E1 cells. American Journal of Physiology. Cell Physiology, 290(6), C1640–1650.
Acknowledgements
The authors acknowledge the technical support of professor Lisiane Bernardi, and the post graduate students of the Federal University of Rio Grande do (UFRGS), Msc. Paloma Santos de Campos, PhD. Bibiana Frazen Matte, Msc. Leonardo Francisco Diel, and Msc. Gabrielle Pedroni.
Funding
No funding was received for conducting this study.
Author information
Authors and Affiliations
Contributions
All authors have contributed to the manuscript. AFS: Concept/Design, Methodology; Data collection, Drafting article, Critical revision of article; EFT: Drafting article, Critical revision of article. MLL: Methodology, Drafting article, Critical revision of article; CMM: Concept/Design, Research supervision, Statistics, Data analysis/interpretation; Critical revision of article.
Corresponding author
Ethics declarations
Conflicts of interest/Competing Interests
The authors declare no conflict of interest.
Ethics Approval
Not applicable.
Consent to Participate
Not applicable.
Consent for publication
The authors authorize the publication of this work.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic Supplementary Material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Soto, A.F., Teixeira, É.F., Lamers, M.L. et al. Effects of Hemin Supplementation on Bone Precursor Cells: An Experimental Study. Stem Cell Rev and Rep 20, 440–442 (2024). https://doi.org/10.1007/s12015-023-10634-x
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12015-023-10634-x