Abstract
Spina bifida is one of the neural tube defects, with a high incidence in human birth defects, which seriously affects the health and quality of life of patients. In the treatment of bone defects, the source of autologous bone is limited and will cause secondary damage to the patient. At the same time, since the bone tissue in animals needs to play a variety of biological functions, its complex structure cannot be replaced by a single material. The combination of mechanical materials and biological materials has become a common choice. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have the advantages of easy access, rapid proliferation, low immunogenicity, and no ethical issues. It is often used in the clinical research of tissue regeneration and repair. Therefore, in this study, we established a spina bifida model using Japanese white rabbits. This model was used to screen the best regenerative repair products for congenital spina bifida, and to evaluate the safety of regenerative repair products. The results showed that the combination of hUC-MSCs with collagen material had better regeneration effect than collagen material alone, and had no negative impact on the health of animals. This study provides a new idea for the clinical treatment of spina bifida, and also helps to speed up the research progress of regenerative repair products.
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Acknowledgements
The authors are very grateful to the animal experiment center of the National Research Institute for Family Planning for its meticulous care of animals.
Funding
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by grants from the National Key Research and Development Program of China [2016YFC1000803].
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XCS, XM and HFX designed the study. HW, XCSand JHL were responsible for the vivo surgery. XCS and HW were responsible for in vitro experiments. HW,XCS and HFX prepared the manuscript. YFY and LQY provided the bone repair materials and collagen membranes. HW, XCS, JHL and HFX were responsible for revising the manuscript critically for important intellectual content. All authors read and approved the final manuscript.
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Ethical approval to report this case was obtained fromthe National Research Institute for Family Planning (Ethics Number2015-16).
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Wang, H., Sun, XC., Li, JH. et al. Combining Bone Collagen Material with hUC-MSCs for Applicationto Spina Bifida in a Rabbit Model. Stem Cell Rev and Rep 19, 1034–1050 (2023). https://doi.org/10.1007/s12015-022-10478-x
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DOI: https://doi.org/10.1007/s12015-022-10478-x