Summary
When in certain culture conditions, organotypic cultures are able to mimic developmental stages of an organ, generating higher-order structures containing functional subunits and progenitor niches. Despite the major advances in the area, researchers have not been able to fully recapitulate the complexity of kidney tissue. Pluripotent stem cells are extensively used in the field, but very few studies make use of adult stem cells. Herein, we describe a simple and feasible method for achieving glomerular epithelial differentiation on an organotypic model comprising human renal progenitor cells from adult kidney (hRPCs). Their glomerular differentiative potential was studied using retinoic acid (RA), a fundamental molecule for intermediate mesoderm induction on early embryogenesis. Immunofluorescence, specific cell surface markers expression and gene expression analysis confirm the glomerular differentiative potential of RA in a short-term culture. We also compared the potential of RA with a potent WNT agonist, CHIR99021, on the differentiative capacity of hRPCs. Gene expression and immunofluorescence analysis confirmed that hRPCs are more sensitive to RA stimulation when compared to CHIR9901. Endothelial cells were also included on the spheroids, resulting in a higher organizational level. The assembly potential of these cells and their selective stimulation will give new insights on adult organotypic cell culture studies and will hopefully guide more works in this important area of research.
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This work was supported by the Portuguese Foundation of Technology (FCT) with the PhD Grant on the Doctoral Program on Advanced Therapies for Health (PATH) (PD/BD/128102/2016) and the the project Cells4_IDs (PTDC/BTM-SAL/28882/2017), under the Compete2020 Funding Program.
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Conceptualization, Rita Sobreiro-Almeida, Maria Elena Melica and Laura Lasagni; Methodology, Rita Sobreiro-Almeida and Maria Elena Melica; Investigation, Rita Sobreiro-Almeida and Maria Elena Melica; Writing – Original Draft, Rita Sobreiro-Almeida; Writing – Review & Editing, Nuno M. Neves and Laura Lasagni; Funding Acquisition, Nuno M. Neves and Paola Romagnani; Resources, Nuno M. Neves and Paola Romagnani; Supervision, Nuno M. Neves, Laura Lasagni and Paola Romagnani.
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Renal progenitor cells were obtained from the digestion of human kidney fragments. These were obtained in agreement with the Ethical Committee on human experimentation of the Azienda Ospedaliero-Universitaria Careggi, Florence, Italy. All subjects gave their informed consent for inclusion before they participated in the study. The study was conducted in accordance with the Declaration of Helsinki, and the protocol was approved by the Ethics Committee on Human Experimentation of the Azienda Ospedaliero-Universitaria Careggi, Florence, Italy (project identification code 2015/0009082 from 25/03/2015).
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Sobreiro-Almeida, R., Melica, M.E., Lasagni, L. et al. Retinoic Acid Benefits Glomerular Organotypic Differentiation from Adult Renal Progenitor Cells In Vitro. Stem Cell Rev and Rep 17, 1406–1419 (2021). https://doi.org/10.1007/s12015-021-10128-8
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DOI: https://doi.org/10.1007/s12015-021-10128-8