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Stem Cell Reviews and Reports

, Volume 14, Issue 3, pp 323–336 | Cite as

Genome Editing in Induced Pluripotent Stem Cells using CRISPR/Cas9

  • Ronen Ben Jehuda
  • Yuval Shemer
  • Ofer Binah
Article

Abstract

The development of the reprogramming technology led to generation of induced Pluripotent Stem Cells (iPSC) from a variety of somatic cells. Ever since, fast growing knowledge of different efficient protocols enabled the differentiation of these iPSCs into different cells types utilized for disease modeling. Indeed, iPSC-derived cells have been increasingly used for investigating molecular and cellular pathophysiological mechanisms underlying inherited diseases. However, a major barrier in the field of iPSC-based disease modeling relies on discriminating between the effects of the causative mutation and the genetic background of these cells. In the past decade, researchers have made great improvement in genome editing techniques, with one of the latest being CRISPR/Cas9. Using a single non-sequence specific protein combined with a small guiding RNA molecule, this state-of-the-art approach enables modifications of genes with high efficiency and accuracy. By so doing, this technique enables the generation of isogenic controls or isogenic mutated cell lines in order to focus on the pathologies caused by a specific mutation. In this article, we review the latest studies combining iPSC and CRISPR/Cas9 technologies for the investigation of the molecular and cellular mechanisms underlying inherited diseases including immunological, metabolic, hematological, neurodegenerative and cardiac diseases.

Keywords

iPSC CRISPR Cas9 

Notes

Compliance with ethical standards

Conflict of interest

None to declare.

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Authors and Affiliations

  1. 1.Department of Physiology, Biophysics and Systems Biology, Rappaport Faculty of MedicineTechnionHaifaIsrael
  2. 2.The Rappaport Institute, Rappaport Faculty of MedicineTechnionHaifaIsrael
  3. 3.Rappaport Faculty of MedicineTechnionHaifaIsrael
  4. 4.Department of BiotechnologyTechnionHaifaIsrael

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