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Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells

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Abstract

Many controversial results exist when comparing mesenchymal stromal cells (MSCs) derived from different sources. Reasons include not only variables in tissue origin, but also methods of cell preparation or choice of expansion media which can strongly influence the expression and hence, function of the cells. In this short report we aimed to investigate the expression of the cell anchoring proteins desmoglein 2, desmocollin 3 and plakophilin 2 in early passage placenta-derived MSCs of fetal (fetal pMSCs) and maternal (maternal pMSCs) origins versus adult bone marrow-derived MSCs (bmMSCs) that were expanded and cultured under the same good manufacturing practice (GMP) conditions. Comprehensive gene expression microarray analysis profiling indicated differential expression of these genes in the different MSC-derived types with fetal pMSCs expressing the highest levels of PKP2, DSC3 and DSG2, followed by maternal pMSCs, while bmMSCs expressed the lowest levels. A higher expression of PKP2 and DSC3 genes in fetal pMSCs was confirmed by qRT-PCR suggesting neonatal increases in the expression of these desmosomal genes vs. adult MSCs. Intracellular desmocollin 3 and desmoglein 2 expression was observed by flow cytometry and cytoplasmic plakophilin 2 by immunofluorescence in all three MSC sources. These data suggest that fetal pMSCs, maternal pMSCs and bmMSCs may anchor intermediate filaments to the plasma membrane via desmocollin 3, desmoglein 2 and plakophilin 2.

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Acknowledgements

The authors thank Tanja Abruzzese and Jan. Maerz for their excellent technical support and expert training of the students involved in this project. This study was funded by the DFG (KFO273) and in part by institutional funding.

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Authors and Affiliations

  1. Laboratory for Cell & Tissue Engineering, Department of Orthopedics and Trauma Surgery, Medical Center - Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg im Breisgau, Germany

    Melanie L. Hart

  2. Clinical Research Group KFO 273, Department of Urology, University of Tubingen Hospital, Tubingen, Germany

    Elisa Rusch, Marvin Kaupp & Wilhelm K. Aicher

  3. Integrative Transcriptomics, Center for Bioinformatics, University of Tübingen, Tübingen, Germany

    Kay Nieselt

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  1. Melanie L. Hart
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Correspondence to Melanie L. Hart.

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Hart, M.L., Rusch, E., Kaupp, M. et al. Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cell Rev and Rep 13, 258–266 (2017). https://doi.org/10.1007/s12015-016-9710-4

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