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High Level P2X7-Mediated Signaling Impairs Function of Hematopoietic Stem/Progenitor Cells

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Abstract

Nucleotides, which bind to P2 receptors, have emerged as a family of mediators in intercellular communication. P2X7 is a member of the P2X family ligand-gated ion channels respond to extracellular ATP. High level expression of P2X7 was detected in leukemia samples, especially in relapsed cases. However, the role of P2X7 mediated signaling in hematopoietic stem/progenitor cells (HSPCs) as well as its potential role in leukemogenesis have not been established. In this study, the expression of P2X7 in hematopoietic cells in different lineages and stages was analyzed. Over-expression of P2X7 in HSPCs was carried out by retrovirus infection to study the impact on HSPCs. The results showed that low level expression of P2X7 was detected in HSPCs. Over-expression of P2X7 in HSPCs resulted in decreased colony forming ability in vitro and engraftment potential in vivo. These results suggested that high level purinergic signaling by P2X7 impaired function of HSPCs.

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Acknowledgments

This work was supported by Grants 81170511, 81370634, 81570153 and 81300376 from the National Natural Science Foundation of China (NSFC); Grants 14JCQNJC10600 from Tianjin Natural Science Foundation; and Graduate Student Innovation Fund from Peking Union Medical College (10023-0710-1021). Z.GG. was a recipient of the New Century Excellent Talents in University (NCET-08-0329).

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Authors and Affiliations

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China

    Wenli Feng, Feifei Yang, Rong Wang, Xiao Yang, Lina Wang, Chong Chen, Jinfeng Liao, Yongmin Lin, Qian Ren & Guoguang Zheng

  2. Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, China

    Guoguang Zheng

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  1. Wenli Feng
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Correspondence to Guoguang Zheng.

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Feng, W., Yang, F., Wang, R. et al. High Level P2X7-Mediated Signaling Impairs Function of Hematopoietic Stem/Progenitor Cells. Stem Cell Rev and Rep 12, 305–314 (2016). https://doi.org/10.1007/s12015-016-9651-y

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