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Stem Cell Reviews and Reports

, Volume 12, Issue 2, pp 189–201 | Cite as

Mechanical Actuation Systems for the Phenotype Commitment of Stem Cell-Based Tendon and Ligament Tissue Substitutes

  • Marco Govoni
  • Claudio Muscari
  • Joseph Lovecchio
  • Carlo Guarnieri
  • Emanuele Giordano
Article

Abstract

High tensile forces transmitted by tendons and ligaments make them susceptible to tearing or complete rupture. The present standard reparative technique is the surgical implantation of auto- or allografts, which often undergo failure.

Currently, different cell types and biomaterials are used to design tissue engineered substitutes. Mechanical stimulation driven by dedicated devices can precondition these constructs to a remarkable degree, mimicking the local in vivo environment. A large number of dynamic culture instruments have been developed and many appealing results collected. Of the cells that have been used, tendon stem cells are the most promising for a reliable stretch-induced tenogenesis, but their reduced availability represents a serious limitation to upscaled production. Biomaterials used for scaffold fabrication include both biological molecules and synthetic polymers, the latter being improved by nanotechnologies which reproduce the architecture of native tendons. In addition to cell type and scaffold material, other variables which must be defined in mechanostimulation protocols are the amplitude, frequency, duration and direction of the applied strain. The ideal conditions seem to be those producing intermittent tension rather than continuous loading. In any case, all physical parameters must be adapted to the specific response of the cells used and the tensile properties of the scaffold. Tendon/ligament grafts in animals usually have the advantage of mechanical preconditioning, especially when uniaxial cyclic forces are applied to cells engineered into natural or decellularized scaffolds. However, due to the scarcity of in vivo research, standard protocols still need to be defined for clinical applications.

Keywords

Ligament Mechanical actuation systems Regenerative medicine Stem cells Tendon Tissue engineering 

Notes

Acknowledgments

This work has been supported by a Regione Emilia Romagna grant: POR-FESR 2007-2011.

Compliance with Ethical Standards

Conflict of Interest

The authors indicate no potential conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Marco Govoni
    • 1
    • 4
  • Claudio Muscari
    • 1
    • 2
  • Joseph Lovecchio
    • 3
  • Carlo Guarnieri
    • 1
    • 2
  • Emanuele Giordano
    • 1
    • 3
  1. 1.BioEngLab, Health Science and Technology – Interdepartmental Center for Industrial Research (HST-CIRI)University of BolognaOzzano EmiliaItaly
  2. 2.Department of Biomedical and Neuromotor Sciences (DIBINEM)University of BolognaBolognaItaly
  3. 3.Laboratory of Cellular and Molecular Engineering “Silvio Cavalcanti” - Department of Electrical, Electronic and Information Engineering (DEI)University of BolognaCesenaItaly
  4. 4.Prometeo Laboratory - Department of Research, Innovation and Technology (RIT)The Rizzoli Orthopedic InstituteBolognaItaly

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