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Stem Cell Reviews and Reports

, Volume 11, Issue 3, pp 526–532 | Cite as

Comparison of Therapeutic Characteristics of Islet Cell Transplantation Simultaneous with Pancreatic Mesenchymal Stem Cell Transplantation in Rats with Type 1 Diabetes Mellitus

  • Ilknur Ozturk UnsalEmail author
  • Zeynep Ginis
  • Ferda Alparslan Pinarli
  • Aynur Albayrak
  • Erman Cakal
  • Mustafa Sahin
  • Tuncay Delibasi
Article

Abstract

Although, pancreas islet call transplantation is a new, promising method for type 1 diabetic patients, it remains as an experimental procedure applied in selected patients. The present study aimed to investigate effect of pancreatic mesenchymal stem cell transplantation simultaneous with islet cell transplantation on islet liveliness and thus on the treatment of diabetes in type 1 diabetic rats. The study used Wistar Albino Rats and was performed in a total of four groups [control (G1), mesenchymal stem cell (G2), islet (G3) and islet + mesencymal stem cell (G4)] each including 8 rats. Blood glucose level of the rats, in which diabetes model has been created using streptozotocin, was measured after 72 h. Blood samples were obtained from the rats 30 days after transplantation and then, their livers and pancreases were kept in 10 % formaldehyde and the experiment was ended. Following staining with H&E, they were morphologically evaluated under a light microscope. Change in mean blood glucose level was statistically significant in G3 and G4 versus G1 and G2 (p = 0.001, p < 0.001, p < 0.001, and p < 0.001 respectively). Histological examination revealed that mean number of islet cells in the pancreases of the rats was higher in G4; difference between the groups was statistically significant (p < 0.001). Transplantation of islet cells together with mesenchymal stem cells showed beneficial effects in terms of prolonging survival of islet grafts suggesting that transplantation of mesenchymal stem cells together with islet cells during clinical islet transplantation may be beneficial in increasing the number of noninsulin-dependent patients in Type 1 diabetes.

Keywords

Islet Mesenchymal stem cell Diabetes mellitus Cell trasplatation Pancreas 

Abbreviations

DM

Diabetes mellitus

MSC

Mesenchymal stem cells

FDA

Fluorescein diacetate

PI

Propidium iodide

DTZ

Dithizone

ILs

Interleukins

FITC

Fluorescein isothiocyanate

IPGTT

Intraperitoneal glucose tolerant test

Notes

Conflicts of interest

There are no conflicts of interest for all authors.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ilknur Ozturk Unsal
    • 1
    Email author
  • Zeynep Ginis
    • 2
  • Ferda Alparslan Pinarli
    • 3
  • Aynur Albayrak
    • 4
  • Erman Cakal
    • 1
  • Mustafa Sahin
    • 5
  • Tuncay Delibasi
    • 1
    • 6
  1. 1.Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Teaching and Research HospitalAnkaraTurkey
  2. 2.Department of Biochemistry, Diskapi Yildirim Beyazit Teaching and Research HospitalAnkaraTurkey
  3. 3.Department of Genetic and Medical Research Center, Diskapi Yildirim Beyazit Teaching and Research HospitalAnkaraTurkey
  4. 4.Department of Pathology, Diskapi Yildirim Beyazit Teaching and Research HospitalAnkaraTurkey
  5. 5.Department of Endocrinology and MetabolismAnkara University School of MedicineAnkaraTurkey
  6. 6.Department of Internal Medicine, School of Medicine (Kastamonu)Hacettepe UniversityAnkaraTurkey

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