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Specific Activity of Electron-Beam Synthesis Immobilized Hyaluronidase on G-CSF Induced Mobilization of Bone Marrow Progenitor Cells

Abstract

The effects of nanotechnology (electron-beam) –PEGylated (or immobilized; Im) hyaluronidase (HD) on the state of the pool of bone marrow progenitor cells and their mobilization induced by granulocyte colony stimulating factor (G-CSF) were studied. A high specific activity of the drug Im-HD on progenitor cells of different classes was demonstrated using parenteral and enteral administration. An increase in the content of erythroid (E), granulomonocytic (GM), fibroblast (F) colony-forming units (CFU) and mesenchymal stem cells (MSC) in bone marrow was shown, as well as G-CSF-induced stimulation of mobilization of precursors into the peripheral blood under the influence of Im-HD. The detected activity of this novel drug on progenitor cells indicates the potential for a safe and highly effective treatment for hematology practice and regenerative medicine.

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Correspondence to M. Yu. Minakova.

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Dygai, A.M., Zyuz’kov, G.N., Zhdanov, V.V. et al. Specific Activity of Electron-Beam Synthesis Immobilized Hyaluronidase on G-CSF Induced Mobilization of Bone Marrow Progenitor Cells. Stem Cell Rev and Rep 9, 140–147 (2013). https://doi.org/10.1007/s12015-012-9423-2

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  • DOI: https://doi.org/10.1007/s12015-012-9423-2

Keywords

  • Progenitor cells
  • Mobilization
  • Immobilization
  • Pegylated hyaluronidase
  • Granulocytic colony stimulating factor
  • Nanotechnology