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Specific Activity of Electron-Beam Synthesis Immobilized Hyaluronidase on G-CSF Induced Mobilization of Bone Marrow Progenitor Cells

Stem Cell Reviews and Reports volume 9, pages 140–147 (2013)Cite this article

Abstract

The effects of nanotechnology (electron-beam) –PEGylated (or immobilized; Im) hyaluronidase (HD) on the state of the pool of bone marrow progenitor cells and their mobilization induced by granulocyte colony stimulating factor (G-CSF) were studied. A high specific activity of the drug Im-HD on progenitor cells of different classes was demonstrated using parenteral and enteral administration. An increase in the content of erythroid (E), granulomonocytic (GM), fibroblast (F) colony-forming units (CFU) and mesenchymal stem cells (MSC) in bone marrow was shown, as well as G-CSF-induced stimulation of mobilization of precursors into the peripheral blood under the influence of Im-HD. The detected activity of this novel drug on progenitor cells indicates the potential for a safe and highly effective treatment for hematology practice and regenerative medicine.

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Authors and Affiliations

  1. Federal State Budgetary Institution, “Research Institute of Pharmacology” under the Siberian Branch of Russian Academy of Medical Sciences, Tomsk city (director - academician of RAMS A.M. Dygai), 634028, Tomsk, Lenin Avenue, 3 (Institute of Pharmacology of RAMS SB), Russia

    A. M. Dygai, G. N. Zyuz’kov, V. V. Zhdanov, E. V. Udut, L. A. Miroshnichenko, E. V. Simanina, T. Yu. Khrichkova, M. Yu. Minakova & P. G. Madonov

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  1. A. M. Dygai
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  2. G. N. Zyuz’kov
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  3. V. V. Zhdanov
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  4. E. V. Udut
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  5. L. A. Miroshnichenko
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  6. E. V. Simanina
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  7. T. Yu. Khrichkova
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  8. M. Yu. Minakova
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  9. P. G. Madonov
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Correspondence to M. Yu. Minakova.

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Dygai, A.M., Zyuz’kov, G.N., Zhdanov, V.V. et al. Specific Activity of Electron-Beam Synthesis Immobilized Hyaluronidase on G-CSF Induced Mobilization of Bone Marrow Progenitor Cells. Stem Cell Rev and Rep 9, 140–147 (2013). https://doi.org/10.1007/s12015-012-9423-2

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  • DOI: https://doi.org/10.1007/s12015-012-9423-2

Keywords

  • Progenitor cells
  • Mobilization
  • Immobilization
  • Pegylated hyaluronidase
  • Granulocytic colony stimulating factor
  • Nanotechnology