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A Novel Stem Cell Tag-Less Sorting Method

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Abstract

Growing interest in stem cell research has led to the development of a number of new methods for isolation. The lack of homogeneity in stem cell preparation blurs standardization, which however is recommended for successful applications. Among stem cells, mesenchymal stem cells (MSCs) are promising candidates for cell therapy applications. This paper presents a fractionation protocol based on a tag-less, flow-assisted method of purifying, distinguishing and sorting MSCs. The protocol entails a suspension of cells in a transport fluid being injected into a ribbon-like capillary device by continuous flow. In a relatively short time (about 30 min) sorted cells are collected. The protocol has been applied to the improvement of MSC isolation, with a specific view to reducing cell manipulation operations, keeping instrumental simplicity and increasing analytical information for cell characterization. Applications such as MSC purification from epithelial contaminants, MSC characterization from various human sources and sorting of MSC subpopulations with high differentiation potential are described. The low cost, full biocompatibility and scale-up potential of the protocol presented could make the procedure attractive for stem cell selection.

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Authors and Affiliations

  1. Department of Chemistry “G. Ciamician”, via Selmi 2, I-40126, Bologna, Italy

    Barbara Roda, Andrea Zattoni, Arianna Di Carlo & Pierluigi Reschiglian

  2. Interuniversity Consortium I.N.B.B., Rome, Italy

    Barbara Roda, Giacomo Lanzoni, Francesco Alviano, Andrea Zattoni, Roberta Costa, Arianna Di Carlo, Cosetta Marchionni, Laura Bonsi, Pierluigi Reschiglian & Gian Paolo Bagnara

  3. Department of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, 40126, Bologna, Italy

    Giacomo Lanzoni, Francesco Alviano, Roberta Costa, Cosetta Marchionni, Laura Bonsi & Gian Paolo Bagnara

  4. Department of Obstetrics and Gynecology, S. Orsola-Malpighi Hospital, University of Bologna, via Massarenti 13, I-40138, Bologna, Italy

    Michele Franchina

  5. Service of Transfusion Medicine, S. Orsola-Malpighi Hospital, I-40138, Bologna, Italy

    Francesca Ricci, Pier Luigi Tazzari & Pasqualepaolo Pagliaro

  6. Department of General Surgery and Organ Transplantation, via Massarenti 9, I-40138, Bologna, Italy

    Sergio Zaccaria Scalinci

Authors
  1. Barbara Roda
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  2. Giacomo Lanzoni
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  3. Francesco Alviano
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  4. Andrea Zattoni
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  5. Roberta Costa
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  6. Arianna Di Carlo
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  7. Cosetta Marchionni
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  8. Michele Franchina
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  9. Francesca Ricci
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  10. Pier Luigi Tazzari
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  11. Pasqualepaolo Pagliaro
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  12. Sergio Zaccaria Scalinci
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  13. Laura Bonsi
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  14. Pierluigi Reschiglian
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  15. Gian Paolo Bagnara
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Corresponding author

Correspondence to Gian Paolo Bagnara.

Additional information

Supported by grants from: AIL-Prato; University of Bologna, RFO 2006.

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Roda, B., Lanzoni, G., Alviano, F. et al. A Novel Stem Cell Tag-Less Sorting Method. Stem Cell Rev and Rep 5, 420–427 (2009). https://doi.org/10.1007/s12015-009-9088-7

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  • DOI: https://doi.org/10.1007/s12015-009-9088-7

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