Abstract
This review focuses on the genomics of mouse hematopoiesis but also draws parallels to other systems and discusses issues common to the analysis of rare populations such as stem cells. As examples from the mouse blood forming system are used to illustrate several points, the authors first give a brief introduction to mouse hematopoiesis as a model system. We review the multiple microarray analyses that have been performed on various mouse hematopoietic subpopulations and comment on both technical and biological aspects of such experiments. The concept of stemness is discussed, and the importance of biological function of gene products, protein-protein interactions and molecular pathways highlighted. Finally, the authors discuss some major unresolved issues in hematopoiesis and discuss the potential uses of future microarray analysis as well as other genomic and functional approaches that might prove useful to further our understanding of hematopoiesis and other stem cell systems.
Similar content being viewed by others
References
Spangrude GJ, Heimfeld S, Issman IL, Science 1988;241:58–62.
Kondo M, Weissman IL, Akashi K, Cell 1997;91:661–672.
Akashi K, Traver D, Miyamoto T, Weissman IL. Nature 2000;404: 193–197
Morrison SJ, Wandycz AM, Hemmati HD, Wright DE, Weissman IL, Development 1997;124:1929–1939.
Christensen JL, Weissman IL, Proc Natl Acad Sci USA 2001;98: 14,541–14,546.
Ivanova NB, Dimos JT, Schaniel C, Hackney JA, Moore KA, Lemischka IR. Science 2002;298:601–604.
Zhong JF, Zhao Y, Sutton S, et al., Proc Natl Acad Sci USA 2005;102: 2448–2453.
Akashi K, He X, Chen J, et al., Blood 2003;101:383–389.
Forsberg EC, Prohaska SS, Katzman S, Heffner GC, Stuart JM, Weissman IL. PloS Genet, 2005;1:e28.
Forsberg EC, Serwold TF, Kogan S, Weissman IL, Passegue E. Submitted.
Passegue E, Wagers AJ, Giuriato S, Anderson WC, Weissman IL. J Exp Med 2005;202:1599–1611.
Cao YA, Wagers AJ, Beilhack A, et al., Proc. Natl Acad Sci USA 2004;101:221–226.
Arai F, Hirao A, Ohmura M, et al., Cell 2004;118:149–161.
Heissig B, Hattori K, Dias S, et al. Cell 2002109:625–637.
Forsberg EC, Karsunky H, Weissman IL In preparation.
Bryder D, Rossi DJ, Weissman IL. Submitted.
Forsberg EC, Prohaska SS Weissman IL, Unpublished Observation.
Hosen N, Weissman IL. submitted.
Ramalho-Santos M, Yoon S, Matsuzaki Y, Mulligan RC, Melton DA. Science 2002;298:597–600.
Evsikov AV, Solter D Science 2003;302:393; author reply 393.
Fortunel NO, Otu HH, Ng HH, et al. Science 2003;302:393; author reply 393.
Jakt LM, Sakurai H, Nishikawa S-I. In preparation.
Fearon DT, Manders P, Wagner SD. Science 2001;293:248–250.
McHeyzer-Williams LJ, McHeyzer-Williams MG. Annu Rev Immunol 2005;23:487–513.
Kaech SM, Hemby S, Kersh E, Ahmed R. Cell 2002;111:837–851.
Luckey CJ, Bhattacharya D, Goldrath AW, Weissman IL, Benoist C, Mathis D, Proc. Natl Acad. Sci USA 2005;103:3304–3309.
Goodman JW, Hodgson GS, Blood 1962;19:702–714.
McCredie KB, Hersh EM, Freireich EJ Science 1971;171:293–294.
Wright DE, Wagers AJ, Gulati AP, Johnson FL, Weissman IL, Science 2001;294:1933–1936.
Passegue E, Wagner EF, Weissman IL, Cell 2004;119:431–443.
Wright DE, Cheshier SH, Wagers AJ, Randall TD, Christensen JL, Weissman IL. Blood 2001;97:2278–2285.
Passegue E, Prohaska SS, Wagers AJ, Forsberg EC, Weissman IL. In preparation.
Rossi DJ, Bryder D, Zhan JM, et al. Proc Natl Acad Sci USA 2005;102:9194–9199.
Diehn M, Sherlock G, Binkley G, et al. Nucleic Acids Res 2003;31: 219–223.
Smyth GK, Speed T. Methods 2003;31:265–273.
Tusher VG, Tibshirani R, Chu G, Proc Natl Acad Sci USA 2001;98: 5116–5121.
Bolstad BM, Irizarry RA, Astrand M, Speed TPA. Bioinformatics 2003;19:185–193.
Paddison PJ, Silva JM, Conklin DS, et al., Nature 2004;428: 427–431.
Silva JM, Li MZ, Chang K, et al. Nat Genet 2005;37:1281–1289.
Eckfeldt CE, Mendenhall EM, Flynn CM, et al., PLoS Biol 2005;3:e254.
Chen CZ, Li L, Lodish HF, Bartel DP. Science 2004;303:83–86.
Barad O, Meiri E, Avniel A, et al. Genome Res 2004;14:2486–2494.
Babak T, Zhang W, Morris Q, Blencowe BJ, Hughes TR, RNA 2004;10:1813–1819.
Liu CG Calin GA, Meloon B, et al. Proc Natl Acad Sci USA 2004;101: 9740–9744.
Sun Y, Koo S, White N, et al. Nucleic Acids Res 2004;32:e188.
Fromont-Racine M, Rain JC, Legrain P. Methods Enzymol 2002;350:513–524.
Ito T, Chiba T, Yoshida M. Trends Biotechnol 2001;19:S23-S27.
Zhong J, Zhang H, Stanyon CA, Tromp G, Finley RL Jr, Genome Res 2003:13:2691–2699.
Fromont-Racine M, Mayes AE, Brunet-Simon A. et al. Yeast 2000; 17:95–110.
Fromont-Racine M, Rain JC, Legrain P. Nat Genet 1997;16:277–282.
Migliaccio AR, Carta C, Migliaccio G. Exp Hematol 1999;27: 1655–1666.
Bhattacharya D, Rossi DJ, Bryder D,Weissman IL. Submitted.
Kuo MH, Allis CD, Methods 1999;19:425–433.
Forsberg EC, Downs KM, Bresnick EH. Blood 2000;96:334–339.
Forsberg EC, Downs KM, Christensen HM, Im H, Nuzzi PA, Bresnick EH, Proc Natl Acad Sci USA 2000;97:14,494–14,499.
Hebbes TR, Thorne AW, Clayton AL, Crane-Robinson C. Nucleic Acids Res 1992;20:1017–1022.
Agalioti T, Chen G, Thanos D, Cell 2002;111:381–392.
Morshead KB, Ciccone DN, Taverna SD, Allis CD, Oettinger MA. Proc Natl Acad Sci USA 2003;100:11,577–11,582.
Su RC, Brown KE, Saaber S, Fisher AG, Merkenschlager M, Smale ST. Nat Genet 2004;36:502–506.
Pokholok DK, Harbison CT, Levine S, et al. Cell 2005;122:517–527.
Liu CL, Kaplan T, Kim M, et al. PLoS Biol 2005;3:e328.
Horak CE, Mahajan MC, Luscombe NM, Gerstein M, Weissman SM, Snyder M. Proc Natl Acad Sci USA 2002;99:2924–2929.
Guenther MG, Jenner RG, Chevalier B, et al. Proc Natl Acad Sci USA 2005;102:8603–8608
Boyer LA, Lee TI, Cole MF, et al. Cell 2005;122:947–956.
Weinmann, AS, Yan PS, Oberley MJ, Huang TH, Farnham PJ. Genes Dev 2002;16:235–244.
Attema JL, Papathanasiou P, Forsberg EC, Weissman IL. In preparation.
Moore KA, Ema H, Lemischka IR, Blood 1997;89:4337–4347.
Nolta JA, Thiemann FT, Arakawa-Hoyt J, et al. Leukemia 2002;16:352–361.
Hackney JA, Charbord P, Brunk BP, Stoeckert CJ, Lemischka IR, Moore KA. Proc Natl Acad Sci USA 2002;99:13,061–13,066.
Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. Cell 2005;121:1109–1121.
Venezia TA, Merchant AA, Ramos CA, et al. PLoS Biol 2004;2:e301.
Terskikh AV, Miyamoto T, Chang C, Diatchenko L, Weissman IL. Blood 2003;102:94–101.
Terskikh AV, Easterday MC, Li L, et al. Proc Natl Acad Sci USA 2001;98:7934–7939.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Forsberg, E.C., Bhattacharya, D. & Weissman, I.L. Hematopoietic stem cells. Stem Cell Rev 2, 23–30 (2006). https://doi.org/10.1007/s12015-006-0005-z
Issue Date:
DOI: https://doi.org/10.1007/s12015-006-0005-z