Abstract
Colorectal cancer (CRC) has emerged as a prevalent malignancy worldwide, exhibiting the high morbidity and mortality rates. Resolvin D1 (RvD1) can exert anti-inflammation and anti-cancer effects on various diseases. This study is aimed to explore the role of RvD1 in CRC cells. HCT15 and SW480 cells were stimulated with IL-6 in our study. A series of assays such as CCK-8, colony formation, wound healing, Transwell, Western blotting, and immunofluorescence staining were designed and conducted to figure out the role of RvD1 in CRC cells. RvD1 suppressed IL-6-induced SW480 and HCT15 cell proliferation. In addition, RvD1 inhibited IL-6-induced SW480 and HCT15 cell migration, invasion, and EMT process. In mechanism, RvD1 inhibited the activation of IL-6/STAT3 signaling in SW480 and HCT15 cells. Angoline strengthened the inhibitive effect of RvD1 on cell malignancy. RvD1 inhibited cell growth, migration, invasion and EMT process by inactivating IL-6/STAT3 signaling in CRC.
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Acknowledgements
We appreciate the support of Huanggang Central Hospital Affiliated to Yangtze University.
Funding
2023 Hunan Provincial People’s Hospital Medical Association Special Research Fund Project (2023YLT010). Hubei University of Science and Technology 2023 Medical Teaching Base Scientific Research Special Fund Project (2023YJDKY02). Scientific Research Project of Hubei Provincial Health and Family Planning Commission in 2021 (WJ2021M083).
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Heng Du and Lijuan You were the main designers of this study. Heng Du, Lijuan You, Anding Wu and Chaowu Chen collected and analyzed the data. Heng Du, Lijuan You, Anding Wu, Fei Wang, Jie Yu and Chaowu Chen drafted the manuscript. All authors read and approved the final manuscript.
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Du, H., You, L., Wu, A. et al. Resolvin D1 Inhibits IL-6-Induced Epithelial-Mesenchymal Transition of Colorectal Cancer Cells by Targeting IL-6/STAT3 Signaling. Cell Biochem Biophys (2024). https://doi.org/10.1007/s12013-024-01299-5
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DOI: https://doi.org/10.1007/s12013-024-01299-5