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Effect of Binding-Affinity and ATPase Activity on the Velocities of Kinesins Using Ratchet Models

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Abstract

We use two-state ratchet models containing single and coupled Brownian motors to understand the role of motor-microtubule binding, ATPase reaction rate and dimerisation on the translational velocities of Kinesin motors. We use model parameters derived from the experimental measurements on KIF1A, KIF13A, KIF13B, and KIF16B motors to compute velocities in μm/s. We observe that both the models show the same trend in velocities (KIF1A > KIF13A > KIF13B > KIF16B) as the experimental results. However, the models significantly underpredict the velocities when compared with the experiments. The predictions of the coupled-motor model are closer to the experiments than those of the single-motor model. Our results indicate that the variation of ATPase reaction rate governs the trend in velocities for the above four motors. The variation of motor-microtubule binding affinity and the coupling strength between the motor domains may only have a secondary effect. More rigorous models that incorporate the power-stroke mechanism are necessary for better quantitative compliance with the experiments.

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Acknowledgements

V.S. acknowledges funding through DBT (Grant No.: BT/PR15214/BRB/10/1449/2015 and BT/RLF/Re-entry/45/2015) and DST-SERB (Grant No.: ECR/2016/000913). P.S. acknowledges funding from DST (Grant No.: SR/WOS-A/LS-73/2017). N.M.P. acknowledges fellowship from IIT Gandhinagar. K.R. acknowledges funding from DST-SERB (Grant No.: ECR/2016/001220). R.M. acknowledges funding from MHRD and IIT Gandhinagar.

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Authors and Affiliations

  1. Chemical Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, Gujarat, 382355, India

    Rupsha Mukherjee & Kaustubh Rane

  2. Biological Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, Gujarat, 382355, India

    Pushpanjali Soppina, Nishaben M. Patel & Virupakshi Soppina

  3. Department of Biotechnology and Bioinformatics, Sambalpur University, Sambalpur, Orissa, 768019, India

    Pushpanjali Soppina

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  1. Rupsha Mukherjee
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Correspondence to Kaustubh Rane.

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Mukherjee, R., Soppina, P., Patel, N.M. et al. Effect of Binding-Affinity and ATPase Activity on the Velocities of Kinesins Using Ratchet Models. Cell Biochem Biophys 80, 31–38 (2022). https://doi.org/10.1007/s12013-021-01057-x

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