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ZFX Facilitates Cell Proliferation and Imatinib Resistance in Chronic Myeloid Leukemia Cells

Cell Biochemistry and Biophysics volume 74, pages 277–283 (2016)Cite this article

Abstract

Zinc finger protein, X-linked (ZFX) mediates the development and progression of human cancers. However, its potential role in chronic myeloid leukemia (CML) is still unknown. The ZFX expression was significantly increased in CML patients and cell lines. Based on loss-of-function experiments in CML cells, we found that knockdown of ZFX expression impaired cell proliferation and induced mitotic arrest in G0/G1 stage and apoptosis. In addition, ZFX silencing sensitized CML cells to imatinib treatment. Further, phospho-Akt (p-Akt), CyclinD1, CyclinE1, and Bcl-2 were downregulated, and Caspase-3 was upregulated in ZFX-silenced cells. In summary, our data suggest that ZFX is a novel oncogene promoting cell proliferation and inducing imatinib resistance via PI3K/Akt signaling pathway. ZFX may represent a potential therapeutic target in CML.

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    Affiliations

    1. Department of Hematology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, 223300, China

      Jingjing Wu, Qian Wang, Yihan Ding, Zhikui Deng, Xueying Lu & Yufeng Li

    2. Department of Oncology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, 223300, China

      Bin Wei

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    1. Jingjing Wu
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    2. Bin Wei
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    Correspondence to Yufeng Li.

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    Jingjing Wu and Bin Wei have contributed equally to this work and should be considered co-first authors.

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    Cite this article

    Wu, J., Wei, B., Wang, Q. et al. ZFX Facilitates Cell Proliferation and Imatinib Resistance in Chronic Myeloid Leukemia Cells. Cell Biochem Biophys 74, 277–283 (2016). https://doi.org/10.1007/s12013-016-0725-x

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    • DOI: https://doi.org/10.1007/s12013-016-0725-x

    Keywords

    • ZFX
    • CML
    • Proliferation
    • Imatinib
    • PI3K/Akt