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Determination of 18 Types of Amino Acids in the Serum of Ischemic Stroke Patients by High Performance Liquid Chromatography-diode Array Detector Derivatized with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate

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Abstract

In this study, we develop a fast and convenient method for the detection of 18 types of free amino acids in the serum of ischemic stroke patients. We use high performance liquid chromatography-diode array detector and thermo C18 column to separate 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate-derivatized amino acids. We find that Gln, His, Gly, Arg, and Cys levels are significantly lower in the serum of ischemic stroke patients comparing with normal persons. Our data indicate that this simple method can be used to detect free amino acids in serum with accurate and reliable results. This study provides a basis for the clinical treatment of ischemic stroke patients and suggests that amino acids can be supplemented to the patients during clinical treatment, which may improve patient prognosis.

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References

  1. Hamidon, B. B., Abdullah, S. A., Zawawi, M. F., Sukumar, N., Aminuddin, A., & Raymond, A. A. (2006). A prospective comparison of percutaneous endoscopic gastrostomy and nasogastric tube feeding in patients with acute dysphagic stroke. Medical Journal of Malaysia, 61(1), 59–66.

    CAS  PubMed  Google Scholar 

  2. Yoo, S. H., Kim, J. S., Kwon, S. U., et al. (2008). Undernutrition as a predictor of poor clinical outcomes in acute ischemic stroke patients. Archives of Neurology, 65(1), 39–43.

    Article  PubMed  Google Scholar 

  3. Hammarqvist, F., Andersson, K., Luo, J. L., et al. (2005). Free amino acid and glutathione concentrations in muscle during short-term starvation and refeeding. Clinical Nutrition, 24(2), 236–243.

    Article  CAS  PubMed  Google Scholar 

  4. Venkatakrishna, K., Kartha, V. B., Pai, K. M., Krishna, C. M., Ravikiran, O., Kurian, J., et al. (2003). HPLC-LIF for early detection of oral cancer. Current Science, 84, 551–557.

    CAS  Google Scholar 

  5. Reddy, Indira, Sherlin, Herald J., Ramani, Pratibha, et al. (2012). Amino acid profile of saliva from patients with oral squamous cell carcinoma using high performance liquid chromatography. Journal of Oral Science, 54(3), 279–283.

    Article  CAS  PubMed  Google Scholar 

  6. Williams, F. N., et al. (2009). Modulation of the hypermetabolic response to trauma: temperature, nutrition, and drugs. Journal of the American College of Surgeons, 208(4), 489–502.

    Article  PubMed Central  PubMed  Google Scholar 

  7. Jiang, Z., Wu, W., et al. (2002). Enteral Nutrition (2nd edn.). Beijing: People’s Medical Publishing House.

    Google Scholar 

  8. Tang, Sung-Chun, Arumugam, Thiruma V., Cutler, Roy G., et al. (2007). Neuroprotective actions of a histidine analogue in models of ischemic stroke. Journal of Neurochemistry, 101(3), 729–736.

    Article  CAS  PubMed  Google Scholar 

  9. Bae, O. N., & Majid, A. (2013). Role of histidine/histamine in carnosine-induced neuroprotection during ischemic brain damage. Brain Research, 1527, 246–254.

    Article  CAS  PubMed  Google Scholar 

  10. Ralph, D. M., Robinson, S. R., Campbell, M. S., & Bishop, G. M. (2010). Histidine, cystine, glutamine, and threonine collectively protect astrocytes from the toxicity of zinc. Free Radical Biology and Medicine, 49(4), 649–657.

    Article  CAS  PubMed  Google Scholar 

  11. Guoyao, Wu, Bazer, Fuller W., Davis, Teresa A., et al. (2009). Arginine metabolism and nutrition in growth, health and disease. Amino Acids, 37(1), 153–168.

    Article  Google Scholar 

  12. Choe, C. U., Atzler, D., Wild, P. S., et al. (2013). Homoarginine levels are regulated by l-arginine: Glycine amidinotransferase and affect stroke outcome: Results from human and murine studies. Circulation, 128(13), 1451–1461.

    Article  CAS  PubMed  Google Scholar 

  13. Padilla-Martin, K., Baltazar-Rendon, B., Gonzalez-Maciel, A., et al. (2009). Effects of glycine on electrical and histological properties of a rat peripheral nerve injury model. Ulus Travma Acil Cerrahi Derg, 15(2), 103–108.

    PubMed  Google Scholar 

  14. Kodama, D., Ono, H., & Tanabe, M. (2011). Increased hippocampal glycine uptake and cognitive dysfunction after peripheral nerve injury. Pain, 152(4), 809–817.

    Article  CAS  PubMed  Google Scholar 

  15. Hong, Dai, Zongcai, Zhang, & Xinshen, Zhang. (2004). Determination methods of amino acids analysis. Leather Science and Engineering, 14(3), 39–43.

    Article  Google Scholar 

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Acknowledgments

We thank Weijun Kang for the assistance with preparation of the experiments. We thank all the patients involved in the study.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Clinical Nutrition, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China

    Ying Xie & Zengning Li

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  1. Ying Xie
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  2. Zengning Li
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Correspondence to Zengning Li.

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Xie, Y., Li, Z. Determination of 18 Types of Amino Acids in the Serum of Ischemic Stroke Patients by High Performance Liquid Chromatography-diode Array Detector Derivatized with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate. Cell Biochem Biophys 73, 111–115 (2015). https://doi.org/10.1007/s12013-015-0648-y

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  • DOI: https://doi.org/10.1007/s12013-015-0648-y

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