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Cell Biochemistry and Biophysics

, Volume 73, Issue 1, pp 93–100 | Cite as

Effect of Low-Intensity Microwave Radiation on Monoamine Neurotransmitters and Their Key Regulating Enzymes in Rat Brain

  • Kanu Megha
  • Pravin S. Deshmukh
  • Alok K. Ravi
  • Ashok K. Tripathi
  • Mahesh P. Abegaonkar
  • Basu D. BanerjeeEmail author
Original Paper

Abstract

The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating enzymes in brain of Fischer rats. Animals were exposed to 900 MHz and 1800 MHz microwave radiation for 30 days (2 h/day, 5 days/week) with respective specific absorption rates as 5.953 × 10−4 and 5.835 × 10−4 W/kg. The levels of monoamine neurotransmitters viz. dopamine (DA), norepinephrine (NE), epinephrine (E) and serotonin (5-HT) were detected using LC–MS/MS in hippocampus of all experimental animals. In addition, mRNA expression of key regulating enzymes for these neurotransmitters viz. tyrosine hydroxylase (TH) (for DA, NE and E) and tryptophan hydroxylase (TPH1 and TPH2) (for serotonin) was also estimated. Results showed significant reduction in levels of DA, NE, E and 5-HT in hippocampus of microwave-exposed animals in comparison with sham-exposed (control) animals. In addition, significant downregulation in mRNA expression of TH, TPH1 and TPH2 was also observed in microwave-exposed animals (p < 0.05). In conclusion, the results indicate that low-intensity microwave radiation may cause learning and memory disturbances by altering levels of brain monoamine neurotransmitters at mRNA and protein levels.

Keywords

Dopamine Epinephrine Microwave Norepinephrine Serotonin Tryptophan hydroxylase Tyrosine hydroxylase 

Notes

Acknowledgments

Authors would like to express their gratitude to Indian Council of Medical Research (ICMR), New Delhi, India for providing the major grant to support the microwave exposure facility. One of the authors, Kanu Megha, Senior Research Fellow is grateful to Department of Science and Technology (DST), Govt. of India for providing INSPIRE Fellowship.

Conflict of interest

The authors report no conflicts of interest.

References

  1. 1.
    Hermann, D. M., & Hossmann, K. A. (1997). Neurological effects of microwave exposure related to mobile communication. Journal of the Neurological Sciences, 152(1), 1–14.CrossRefPubMedGoogle Scholar
  2. 2.
    Lai, H. (1994). Neurological effects of radiofrequency electromagnetic radiation. In J. C. Lin (Ed.), Advances in electromagnetic fields in living systems (Vol. 1, pp. 27–80). New York: Plenum Press.CrossRefGoogle Scholar
  3. 3.
    Preece, A. W., Iwi, G., Davies-Smith, A., Wesnes, K., Butler, S., Lim, E., & Varey, A. (1999). Effect of a 915 MHz simulated mobile phone signal on cognitive function in man. International Journal of Radiation Biology, 75(4), 447–456.CrossRefPubMedGoogle Scholar
  4. 4.
    Koivisto, M., Revonsuo, A., Krause, C. M., Haarala, C., Sillanmaki, L., Laine, M., & Hamalainen, H. (2000). Effects of 902 MHz electromagnetic field emitted by cellular telephones on response times in humans. NeuroReport, 11(2), 413–415.CrossRefPubMedGoogle Scholar
  5. 5.
    Koivisto, M., Krause, C. M., Revonsuo, A., Laine, M., & Hamalainen, H. (2000). The effects of electromagnetic field emitted by GSM phones on working memory. NeuroReport, 11(8), 1641–1643.CrossRefPubMedGoogle Scholar
  6. 6.
    Haarala, C., Ek, M., Bjornberg, L., Laine, M., Revonsuo, A., Koivsto, M., & Hamalainen, H. (2004). 902 MHz mobile phone does not affect short term memory in humans. Bioelectromagnetics, 25(6), 452–456.CrossRefPubMedGoogle Scholar
  7. 7.
    Marier, M., Blackemore, C., & Lovisto, M. (2000). The health hazards of mobile phones. British Medical Journal, 320(7245), 1288–1289.CrossRefGoogle Scholar
  8. 8.
    Chia, S. E., Chia, H. P., & Tan, J. S. (2000). Prevalence of headache among handheld cellular telephone users in Singapore: A community study. Environmental Health Perspectives, 108(11), 1059–1062.PubMedCentralCrossRefPubMedGoogle Scholar
  9. 9.
    De luliis, G. N., Newey, R. J., King, B. V., & Aitken, R. J. (2009). Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro. PLoS ONE, 4(7), e6446.CrossRefGoogle Scholar
  10. 10.
    Megha, K., Deshmukh, P. S., Banerjee, B. D., Tripathi, A. K., & Abegaonkar, M. P. (2012). Microwave radiation induced oxidative stress, cognitive impairment and inflammation in brain of Fischer rats. Indian Journal of Experimental Biology, 50, 889–896.PubMedGoogle Scholar
  11. 11.
    Deshmukh, P. S., Megha, K., Banerjee, B. D., Abegaonkar, M. P., Ahmed, R. S., & Tripathi, A. K. (2013). Detection of low level microwave radiation induced DNA damage vis a vis genotoxicity in Fischer rat brain. Toxicology International, 20(1), 19–24.PubMedCentralCrossRefPubMedGoogle Scholar
  12. 12.
    Chance, W. T., Grossman, C. J., Newrock, R., Bovin, G., Yerian, S., Schmitt, G., & Mendenhall, C. (1995). Effects of electromagnetic fields and gender on neurotransmitters and amino acids in rats. Physiology & Behavior, 58(4), 743–748.CrossRefGoogle Scholar
  13. 13.
    Deshmukh, P. S., Megha, K., Banerjee, B. D., Abegaonkar, M. P., Ahmed, R. S., Tripathi, A. K., & Mediratta, P. K. (2012). Modulation of heat shock protein level and cognitive impairment in Fischer rats exposed to low level microwave radiation. Asiatic Journal of Biotechnology Resources, 3(10), 1391–1399.Google Scholar
  14. 14.
    Zhao, L., Peng, R. Y., Wang, S. M., Wang, L. F., Gao, Y. B., Dong, J., et al. (2012). Relationship between cognition function and hippocampus structure after long-term microwave exposure. Biomedical and Environmental Sciences, 25(2), 182–188.PubMedGoogle Scholar
  15. 15.
    Ardoino, L., Lopresto, V., Mancini, S., Marino, C., Pinto, R., & Lovisolo, G. A. (2005). A radio-frequency system for in vivo pilot experiments aimed at the studies on biological effects of electromagnetic fields. Physics in Medicine & Biology, 50(15), 3643–3654.CrossRefGoogle Scholar
  16. 16.
    Livak, J. K., & Schmittgen, D. T. (2001). Analysis of relative gene expression data using real time quantitative PCR and the 2−∆∆CT method. Methods, 25(4), 402–408.CrossRefPubMedGoogle Scholar
  17. 17.
    Wu, Y., Jia, Y., Guo, Y., & Zheng, Z. (1999). Influence of EMP on the nervous system of rats. Acta Biophysica Sinica, 15, 152–157.Google Scholar
  18. 18.
    Sakatani, S., & Hirose, A. (2002). A quantitative evaluation of the magnetic field generated by a CA3 pyramidal cell at EPSP and action potential stages. IEEE Transactions on Biomedical Engineering, 49(4), 310–319.CrossRefPubMedGoogle Scholar
  19. 19.
    Salford, L. G., Brun, A. E., Eberhardt, J. L., Malmgren, L., & Persson, B. R. (2003). Nerve cell damage in mammalian brain after exposure to microwaves from GSM mobile phones. Environmental Health Perspectives, 111(7), 881–883.PubMedCentralCrossRefPubMedGoogle Scholar
  20. 20.
    Manikonda, P. K., Rajendra, P., Devendranath, D., Gunasekaran, B., Channakeshava, Aradhya, R. S., et al. (2007). Influence of extremely low frequency magnetic fields on Ca2+ signaling and NMDA receptor functions in rat hippocampus. Neuroscience Letters, 413(2), 145–149.CrossRefPubMedGoogle Scholar
  21. 21.
    Kobayashi, K. (2001). Role of catecholamine signaling in brain and nervous system functions: New insights from mouse molecular genetic study. Journal of Investigative Dermatology Symposium Proceedings, 6(1), 115–121.CrossRefGoogle Scholar
  22. 22.
    Lovinger, D. M. (1999). The role of serotonin in alcohol’s effects on the brain. Current Separations, 18(1), 23–28.Google Scholar
  23. 23.
    Xu, F., Gao, M., Wang, L., & Jin, L. (2002). Study on the effect of electromagnetic impulse on neurotransmitter metabolism in nerve cells by high-performance liquid chromatography-electrochemical detection coupled with microdialysis. Analytical Biochemistry, 307(1), 33–39.CrossRefPubMedGoogle Scholar
  24. 24.
    Jing, J., Yuhua, Z., Xiao-qian, Y., Rongping, J., Dong-mei, G., & Xi, C. (2012). The influence of microwave radiation from cellular phone on fetal rat brain. Electromagnetic Biology and Medicine, 31(1), 57–66.CrossRefPubMedGoogle Scholar
  25. 25.
    Merritt, J. H., Chamnes, A. F., Hartzell, R. H., & Allan, S. J. (1977). Orientation effect on microwave-induced hyperthermia and neurochemical correlates. Journalof Microwave Power, 12(2), 167–172.Google Scholar
  26. 26.
    Snyder, S. H., (1971). The effect of microwave irradiation on the turn over rate of serotonin and norepinephrine and the effect of microwave metabolizing enzymes. Washington, DC: U.S Army Medical Research and Development Command. Final Report, Contract No. DADA 17-69-C-9144.Google Scholar
  27. 27.
    Belyaev, I. Y., Koch, C. B., Terenius, O., Roxström-Lindquist, K., Malmgren, L. O., Sommer, W. H., et al. (2006). Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation. Bioelectromagnetics, 27(4), 295–306.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Kanu Megha
    • 1
  • Pravin S. Deshmukh
    • 1
  • Alok K. Ravi
    • 2
  • Ashok K. Tripathi
    • 1
  • Mahesh P. Abegaonkar
    • 3
  • Basu D. Banerjee
    • 1
    Email author
  1. 1.Environmental Biochemistry and Molecular Biology Laboratory, Department of BiochemistryUniversity College of Medical Sciences & G.T.B. Hospital (University of Delhi)New DelhiIndia
  2. 2.Dr. R. P. Centre for Ophthalmic Sciences, Department of Ocular BiochemistryAll India Institute of Medical SciencesNew DelhiIndia
  3. 3.Centre for Applied Research in Electronics (CARE)Indian Institute of TechnologyNew DelhiIndia

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