Effect of Low-Intensity Microwave Radiation on Monoamine Neurotransmitters and Their Key Regulating Enzymes in Rat Brain
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The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating enzymes in brain of Fischer rats. Animals were exposed to 900 MHz and 1800 MHz microwave radiation for 30 days (2 h/day, 5 days/week) with respective specific absorption rates as 5.953 × 10−4 and 5.835 × 10−4 W/kg. The levels of monoamine neurotransmitters viz. dopamine (DA), norepinephrine (NE), epinephrine (E) and serotonin (5-HT) were detected using LC–MS/MS in hippocampus of all experimental animals. In addition, mRNA expression of key regulating enzymes for these neurotransmitters viz. tyrosine hydroxylase (TH) (for DA, NE and E) and tryptophan hydroxylase (TPH1 and TPH2) (for serotonin) was also estimated. Results showed significant reduction in levels of DA, NE, E and 5-HT in hippocampus of microwave-exposed animals in comparison with sham-exposed (control) animals. In addition, significant downregulation in mRNA expression of TH, TPH1 and TPH2 was also observed in microwave-exposed animals (p < 0.05). In conclusion, the results indicate that low-intensity microwave radiation may cause learning and memory disturbances by altering levels of brain monoamine neurotransmitters at mRNA and protein levels.
KeywordsDopamine Epinephrine Microwave Norepinephrine Serotonin Tryptophan hydroxylase Tyrosine hydroxylase
Authors would like to express their gratitude to Indian Council of Medical Research (ICMR), New Delhi, India for providing the major grant to support the microwave exposure facility. One of the authors, Kanu Megha, Senior Research Fellow is grateful to Department of Science and Technology (DST), Govt. of India for providing INSPIRE Fellowship.
Conflict of interest
The authors report no conflicts of interest.
- 13.Deshmukh, P. S., Megha, K., Banerjee, B. D., Abegaonkar, M. P., Ahmed, R. S., Tripathi, A. K., & Mediratta, P. K. (2012). Modulation of heat shock protein level and cognitive impairment in Fischer rats exposed to low level microwave radiation. Asiatic Journal of Biotechnology Resources, 3(10), 1391–1399.Google Scholar
- 17.Wu, Y., Jia, Y., Guo, Y., & Zheng, Z. (1999). Influence of EMP on the nervous system of rats. Acta Biophysica Sinica, 15, 152–157.Google Scholar
- 22.Lovinger, D. M. (1999). The role of serotonin in alcohol’s effects on the brain. Current Separations, 18(1), 23–28.Google Scholar
- 23.Xu, F., Gao, M., Wang, L., & Jin, L. (2002). Study on the effect of electromagnetic impulse on neurotransmitter metabolism in nerve cells by high-performance liquid chromatography-electrochemical detection coupled with microdialysis. Analytical Biochemistry, 307(1), 33–39.CrossRefPubMedGoogle Scholar
- 25.Merritt, J. H., Chamnes, A. F., Hartzell, R. H., & Allan, S. J. (1977). Orientation effect on microwave-induced hyperthermia and neurochemical correlates. Journalof Microwave Power, 12(2), 167–172.Google Scholar
- 26.Snyder, S. H., (1971). The effect of microwave irradiation on the turn over rate of serotonin and norepinephrine and the effect of microwave metabolizing enzymes. Washington, DC: U.S Army Medical Research and Development Command. Final Report, Contract No. DADA 17-69-C-9144.Google Scholar
- 27.Belyaev, I. Y., Koch, C. B., Terenius, O., Roxström-Lindquist, K., Malmgren, L. O., Sommer, W. H., et al. (2006). Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation. Bioelectromagnetics, 27(4), 295–306.CrossRefPubMedGoogle Scholar