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Hypoxia-Inducible Factor 1-Alpha Regulates Cancer-Inhibitory Effect of Human Mesenchymal Stem Cells

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Abstract

Mesenchymal stem cells (MSCs) have been shown to be able to inhibit cancer cells growth. In this study, we investigate the role and the molecular mechanism of hypoxia-inducible factor 1-alpha (HIF-1α) in inhibition of cancer cell proliferation by human MSCs through depletion and overexpression of HIF-1α in human MSCs. We found that the cell culture medium from HIF-1α-depleted Z3 cells significantly promotes breast cancer MCF-7 cell proliferation and colony formation. The expression of p21 is increased in MCF-7 cells, but p53 level remains unchanged. In contrast, the cultured medium from HIF-1α-overexpressed Z3 cells dramatically inhibits MCF-7 cell proliferation and colony formation. The expression of p21 is inhibited in MCF-7 cells, but p53 does not change. We conclude HIF-1α promotes inhibitory effect of human MCSs on breast cancer cell proliferation and colony formation. This process is tightly correlated with cell cycle protein p21 level in cancer cells.

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Authors and Affiliations

  1. Institute of Orthopedics, The First Affiliated Hospital of Chinese PLA General Hospital, 51 Fucheng Road, Haidian District, Beijing, 100048, China

    Yuan Hu, Shu-Xun Hou, Jin-Shu Tang, Xiu-Xiu Shi, Jiang Qin & Neng Ren

  2. Department of Cardiology, The First Affiliated Hospital of Chinese PLA General Hospital, 51 Fucheng Road, Haidian District, Beijing, 100048, China

    Jing Bai

Authors
  1. Yuan Hu
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  2. Jing Bai
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  3. Shu-Xun Hou
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  4. Jin-Shu Tang
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  5. Xiu-Xiu Shi
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  6. Jiang Qin
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  7. Neng Ren
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Correspondence to Yuan Hu.

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Yuan Hu and Jing Bai have contributed equally to the work.

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Hu, Y., Bai, J., Hou, SX. et al. Hypoxia-Inducible Factor 1-Alpha Regulates Cancer-Inhibitory Effect of Human Mesenchymal Stem Cells. Cell Biochem Biophys 72, 131–136 (2015). https://doi.org/10.1007/s12013-014-0420-8

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  • DOI: https://doi.org/10.1007/s12013-014-0420-8

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