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Changes of Serum Osteocalcin, Calcium, and Potassium in a Rat Model of Type 2 Diabetes

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Abstract

Changes in serum levels of calcium (Ca), phosphorus (P), Ca phosphate, alkaline phosphatase (AKP), and osteocalcin (BGP) were assessed in a model of type 2 diabetes in 8-weeks-old Sprague–Dawley rats. Throughout the study, the animals in Group A consumed high-carbohydrate, high-fat diet and were administered two intraperitoneal injections of streptozocin (STZ) at 4 (25 mg/kg) and 8 (40 mg/kg) weeks after diet initiation. Animals in Group B received similar diet but intraperitoneal injections with citrate buffer. The control group rats (Group C) were fed normal diet and received citrate buffer injections. The Ca levels in Group A were significantly decreased at 12 and 16 weeks, while P levels were significantly lower at 8, 12, and 16 weeks after the first STZ injection (p < 0.05 vs. Groups B and C). The Ca phosphate levels in Group A were significantly lower at 16 weeks (p < 0.05 vs. Groups B and C). There was a discrepancy in kinetics of AKP and BGP levels in Group A, such that the former showed a significant surge at 8 weeks (p < 0.05 vs. Groups B and C) followed by a decline at 16 weeks (p < 0.05 vs. Groups B and C), while the levels of the latter consistently decreased and became significantly decreased at 12 and 16 weeks (p < 0.05 vs. Groups B and C). In conclusion, these findings may lead to better understanding of the pathogenesis of osteoporosis in diabetic rats.

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Abbreviations

STZ:

Streptozocin

BGP:

Bone gla-containing protein (osteocalcin)

AKP:

Alkaline phosphatase

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Authors and Affiliations

  1. Department of Clinical Laboratory, First Affiliated Hospital of Yangtze University, jingzhou, 434000, Hubei, China

    Lin Xiao, Xian Mo Wang, Tao Yang, Ying Xiong, Zhong Gui Zhang, Jun Ding, Chen Xu & Chang Yuan Xiong

  2. Hubei University of Chinese Medicine, 1 Huangjiahu West, Wuhan, 430065, Hube, China

    Lin Xiao, Chen Xu & Chang Yuan Xiong

Authors
  1. Lin Xiao
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  2. Xian Mo Wang
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  3. Tao Yang
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  4. Ying Xiong
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  5. Zhong Gui Zhang
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  6. Jun Ding
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  7. Chen Xu
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  8. Chang Yuan Xiong
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Corresponding authors

Correspondence to Xian Mo Wang or Chang Yuan Xiong.

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Lin Xiao and Chen Xu contributed equally to the manuscript and should be considered co-first authors

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Xiao, L., Wang, X.M., Yang, T. et al. Changes of Serum Osteocalcin, Calcium, and Potassium in a Rat Model of Type 2 Diabetes. Cell Biochem Biophys 71, 437–440 (2015). https://doi.org/10.1007/s12013-014-0220-1

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  • DOI: https://doi.org/10.1007/s12013-014-0220-1

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