Abstract
The present study was conducted to explore the protective effect of exogenous gelsolin (GSN) in mice exposed to high-dose of radiation. Changes in the levels of GSNs in peripheral blood of mice and cytoplasm of cultured human intestinal epithelial cells (HIECs) were analyzed after their exposure to different doses of 137Cs γ-rays at a fixed dose rate. The coagulation associated indices, such as prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured. Effect on radiation-mediated oxidative damage was evaluated by estimating the altered glutathione (GSH) and malondialdehyde (MDA) concentrations in the blood. The results showed that radiation induced a pronounced decrease in the pGSN blood levels. However, the cGSN levels of irradiated HIECs were increased in a dose-dependent manner. Administration of recombinant human pGSN to irradiated mice resulted in an ameliorated clotting time as indicated by the PT and the APTT indices. The treatment of mice with hpGSN enhanced the blood levels of GSH while MDA concentrations were decreased indicating an improved antioxidant status. These results suggest that GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure.
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Acknowledgments
The research was supported by the National Natural Science Fund 81372932 and Army Force Fund BWS11J009. We thank Dr.Po-shun Lee (Brigham and Women's hospital, Boston, MA) for valuable suggestions and providing us with the recombiant human gelsolin and also thank Dr. Thomas P.Stossel(Brigham and Women's Hospital, Boston, MA) and Susan Goelz (Biogen,Cambridge, MA) for their interest in this work and for providing useful advice.
Conflict of interest
The authors declare that they have no competing interests, including, but not limited to, conflicts of interest regarding the gift of gelsolin.
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Mingjuan Li and Fengmei Cui have contributed equally to this work.
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Li, M., Cui, F., Cheng, Y. et al. Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury. Cell Biochem Biophys 71, 389–396 (2015). https://doi.org/10.1007/s12013-014-0210-3
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DOI: https://doi.org/10.1007/s12013-014-0210-3