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The Adhesion AND Growth of Both the Human Primary Gingival Epithelial Cells and Streptococcus Mutans on Micro-Arc Oxidized Titanium

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Abstract

With good osseointegration properties, micro-arc oxidation has now gradually become the key point in basic research and clinical trials, but interface between the implant surface treated by micro-arc oxidation and gingival soft tissues has been seldom reported. The influences of micro-arc oxidation surface treatment on the biological behavior of primary human gingival epithelial cells (hGEC) and common pathogen Streptococcus mutans have been studied. MTT method was taken to test the adhesion and growth of hGEC on different treated surfaces. No significant changes were found between with or without MAO- treated surface. However, higher growth rate was observed in MAO group at first and third days, although it showed no significant difference at fifth and seventh day. Secretions of EGF of the cells grown on both surfaces were also no big changes (P > 0.05). RT-PCR showed adhesion gene of hGEC, E-cad on the first day of micro-arc oxidation surface treatment, expression level is higher than that of polishing group (P < 0.05), but no significant difference of the expression levels of Itgβ1, PCNA, and EGF. Finally, easier adhesion and high growth rate of Streptococcus mutans were found at MAO-treated surface (P < 0.05). In conclusion, our data suggested MAO-treated Ti surface may favor epithelial cell adhesion, but it also increase the risk of bacterial infection.

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Authors and Affiliations

  1. State Key Laboratory of Military Stomatology, Department of Dental Implant, School of Stomatology, The Fourth Military Medical University, Chang Le Road 145, Xi’an, People’s Republic of China

    Jiangning Lv & Baolin Liu

  2. Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi’an, China

    Hongwei Li

  3. Department of Nutrition, 302 Hospital of PLA, Beijing, 100039, China

    Ying Mu

  4. Department of Internal Medicine, The Fourth Hospital of Baoding City Hebei Province, Baoding, China

    ShuMin Wang

  5. Department of Stomatology, 261 Hospital of PLA, Beijing, China

    Jing Sun

  6. Department of Emergency, The First People’s Hospital of Huoqiu County Anhui Province, Huoqiu, 237400, China

    Jun Jiang

  7. Department of Dermatology, The First People’s Hospital of Huoqiu County Anhui Province, Huoqiu, 237400, China

    Ping Ding

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  1. Jiangning Lv
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  2. Hongwei Li
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  3. Ying Mu
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  4. ShuMin Wang
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  6. Jun Jiang
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  8. Baolin Liu
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Corresponding author

Correspondence to Baolin Liu.

Additional information

Jiangning Lv and Hongwei Li contributed equally to this work.

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Lv, J., Li, H., Mu, Y. et al. The Adhesion AND Growth of Both the Human Primary Gingival Epithelial Cells and Streptococcus Mutans on Micro-Arc Oxidized Titanium. Cell Biochem Biophys 70, 1083–1090 (2014). https://doi.org/10.1007/s12013-014-0026-1

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  • DOI: https://doi.org/10.1007/s12013-014-0026-1

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