Skip to main content
SpringerLink
Log in

Optimization of Extraction and Purification of Arctiin from Fructus arctii and Its Protection Against Glucose-Induced Rat Aortic Endothelial Cell Injury

  • Original Paper
  • Published:
Cell Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

To develop an efficient method for extracting and purifying the active ingredient, arctiin, from Fructus arctii and to investigate the protective effect of arctiin against glucose-induced rat aortic endothelial cell (RAEC) injury was investigated. Using a L9 (34) orthogonal array and two-step column chromatography (with AB-8 macroporous resin) arctiin extraction was optimized using a reflux method with 70 % ethanol. The RAECs were then treated with different concentrations of arctiin (1, 10, or 100 μg/ml). The effects of arctiin on cell viability in a high glucose medium, malondialdehyde (MDA) levels, and lactate dehydrogenase were measured using commercially available assays. After extraction, the purity of arctiin reached 95.7 %. In rats, arctiin was shown to stimulate the proliferation of RAECs in a high glucose medium in a dose-dependent manner. Exposure of RAECs to high glucose resulted in a significant increase in MDA and release of lactate dehydrogenase. This was accompanied by significant increase in nitric oxide release and expression of antiendothelial nitric oxide synthase. This technique resulted in relatively pure arctiin extraction. Furthermore, the results from this study suggest that arctiin could potentially function as a protector against vascular endothelial cell injury and further investigation is warranted.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Davignon, J., & Ganz, P. (2004). Role of endothelial dysfunction in atherosclerosis. Circulation, 109, III27–III32.

    Article  PubMed  Google Scholar 

  2. Potenza, M. A., Gagliardi, S., Nacci, C., Carratu, M. R., & Montagnani, M. (2009). Endothelial dysfunction in diabetes: From mechanisms to therapeutic targets. Current Medicinal Chemistry, 16, 94–112.

    Article  CAS  PubMed  Google Scholar 

  3. Ribau, J. C., Hatton, M. W., & Richardson, M. (1998). Changes in the aortic endothelium and plasma von Willebrand factor levels during the onset and progression of insulin-dependent diabetes in BB rats. Atherosclerosis, 139, 291–299.

    Article  CAS  PubMed  Google Scholar 

  4. Meyers, M. R., & Gokce, N. (2007). Endothelial dysfunction in obesity: Etiological role in atherosclerosis. Current Opinion in Endocrinology Diabetes and Obesity, 14, 365–369.

    Article  CAS  Google Scholar 

  5. Neutel, J. M. (2000). Endothelial dysfunction: Its pathogenic role in atherosclerosis and its reversal by ACE inhibition. Postgraduate Medicine, 2000(108), 4–11.

    Google Scholar 

  6. Ren, C. S., & Zhu, Q. L. (2003). Research overview of Fructus arctii. Journal of Medicine and Pharmacy of Chinese Minorities, 2003, 36–39.

    Google Scholar 

  7. Gong, Y. M., Liu, L. G., & Gao, N. (2005). Study of the fruits of Arctium lappa L. Strait Pharmaceutical Journal, 2005, 1–4.

    Google Scholar 

  8. Mi, J. Y., & Song, C. Q. (2002). Anti-tumor and immunological activity of lignans from Fructus arctii. Lishizhen Medicine and Materia Medica Research, 2002, 168–169.

    Google Scholar 

  9. Wang, H. Y., & Chen, Y. P. (2004). Clinical observation on treatment of diabetic nephropathy with compound Fructus arctii mixture. Zhongguo Zhong Xi Yi Jie He Za Zhi, 24, 589–592.

    PubMed  Google Scholar 

  10. Xu, Z., Wang, X., Zhou, M., Ma, L., Deng, Y., Zhang, H., et al. (2008). The antidiabetic activity of total lignan from Fructus arctii against alloxan-induced diabetes in mice and rats. Phytotherapy Research, 22, 97–101.

    Article  CAS  PubMed  Google Scholar 

  11. Wang, L., Zhao, F., & Liu, K. (2008). Advances in studies on pharmacological effect of arctiin and arctigenin. Chinese Traditional and Herbal Drugs, 2008, 467–470.

    Google Scholar 

  12. Wang, H. Y., & Yang, J. S. (1993). Studies on the chemical constituents of Arctium lappa L. Yao Xue Xue Bao, 28, 911–917.

    CAS  PubMed  Google Scholar 

  13. Wang, J., Hou, F. F., Kang, T. G., Li, C., & Lin, Y. J. (2002). Preparation and purification of arctiin. Chinese Traditional and Herbal Drugs, 2002, 467–470.

    Google Scholar 

  14. Andre, P., Michel, M., Schott, C., & Stoclet, J. C. (1992). Characterization of cultured rat aortic endothelial cells. Journal of Physiology Paris, 86, 177–184.

    Article  CAS  Google Scholar 

  15. Cole, O. F., Fan, T. P., & Lewis, G. P. (1986). Isolation, characterisation, growth and culture of endothelial cells from the rat aorta. Cell Biology International Reports, 10, 399–405.

    Article  CAS  PubMed  Google Scholar 

  16. Mahabeleshwar, G. H., Somanath, P. R., & Byzova, T. V. (2006). Methods for isolation of endothelial and smooth muscle cells and in vitro proliferation assays. Methods in Molecular Medicine, 129, 197–208.

    PubMed  Google Scholar 

  17. Dai, Z., Liao, D. F., Jiang, D. J., Deng, H. W., & Li, Y. J. (2004). 3,4,5,6-Tetrahydroxyxanthone prevents vascular endothelial cell apoptosis induced by high glucose. Naunyn Schmiedebergs Archives of Pharmacology, 370, 314–319.

    Article  CAS  Google Scholar 

  18. Dubuisson, M. L., de Wergifosse, B., Trouet, A., Baguet, F., Marchand-Brynaert, J., & Rees, J. F. (2000). Antioxidative properties of natural coelenterazine and synthetic methyl coelenterazine in rat hepatocytes subjected to tert-butyl hydroperoxide-induced oxidative stress. Biochemical Pharmacology, 60, 471–478.

    Article  CAS  PubMed  Google Scholar 

  19. Figueira, M. E., Barroso, M. I., Rico, J. M., & Castro, M. (1998). Dietary beta-adrenoceptor agonists have a persistent effect on nitric oxide synthesis in rat cultured smooth muscle cells. European Journal of Pharmacology, 362, 261–266.

    Article  CAS  PubMed  Google Scholar 

  20. Pacheco, M. E., Beltran, A., Redondo, J., Manso, A. M., Alonso, M. J., & Salaices, M. (2006). High glucose enhances inducible nitric oxide synthase expression. Role of protein kinase C-betaII. European Journal of Pharmacology, 538, 115–123.

    Article  CAS  PubMed  Google Scholar 

  21. Wang, X., Li, F., Sun, Q., Yuan, J., & Zheng, C. (2005). Application of preparative high-speed counter-current chromatography for separation and purification of arctiin from Fructus arctii. Journal of Chromatography A, 1063, 247–251.

    Article  CAS  PubMed  Google Scholar 

  22. Guo, M., Liang, J., & Wu, S. (2010). On-line coupling of counter-current chromatography and macroporous resin chromatography for continuous isolation of arctiin from the fruit of Arctium lappa L. Journal of Chromatography A, 1217, 5398–5406.

    Article  CAS  PubMed  Google Scholar 

  23. Dong, J., Liu, Y., Liang, Z., & Wang, W. (2010). Investigation on ultrasound-assisted extraction of salvianolic acid B from Salvia miltiorrhiza root. Ultrasonics Sonochemistry, 17, 61–65.

    Article  CAS  PubMed  Google Scholar 

  24. Jun, X. (2009). Caffeine extraction from green tea leaves assisted by high pressure processing. Journal of Food Engineering, 94, 105–109.

    Article  CAS  Google Scholar 

  25. Minamino, T., Miyauchi, H., Yoshida, T., Ishida, Y., Yoshida, H., & Komuro, I. (2002). Endothelial cell senescence in human atherosclerosis: Role of telomere in endothelial dysfunction. Circulation, 105, 1541–1544.

    Article  CAS  PubMed  Google Scholar 

  26. Prasad, A., Zhu, J., Halcox, J. P., Waclawiw, M. A., Epstein, S. E., & Quyyumi, A. A. (2002). Predisposition to atherosclerosis by infections: Role of endothelial dysfunction. Circulation, 106, 184–190.

    Article  PubMed  Google Scholar 

  27. Zhang, W., Wang, Y., Yang, Z., Qiu, J., Ma, J., Zhao, Z., et al. (2011). Antioxidant treatment with quercetin ameliorates erectile dysfunction in streptozotocin-induced diabetic rats. Journal of Bioscience and Bioengineering, 112, 215–218.

    Article  CAS  PubMed  Google Scholar 

  28. Kade, I. J., Borges, V. C., Savegnago, L., Ibukun, E. O., Zeni, G., Nogueira, C. W., et al. (2009). Effect of oral administration of diphenyl diselenide on antioxidant status, and activity of delta aminolevulinic acid dehydratase and isoforms of lactate dehydrogenase, in streptozotocin-induced diabetic rats. Cell Biology and Toxicology, 25, 415–424.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Grants from the Chinese National Natural Science Foundation (Grant No. 30973908) and the Natural Science Foundation of Chongqing (CSTC, 2009AC5025).

Author information

Authors and Affiliations

  1. Institute of Respiratory Institute for Respiratory Disease, The Second Affiliated Hospital of Third Military Medical University, Chongqing, 400042, People’s Republic of China

    Lai-chun Lu & Gui-sheng Qian

  2. Institute of Cardiovascular Disease, The Second Affiliated Hospital of Third Military Medical University, Chongqing, People’s Republic of China

    Ming-bao Song

  3. Institute of Pharmacy, Third Military Medical University, Chongqing, People’s Republic of China

    Shi-wen Zhou

  4. Department of Pharmacy, The Second Affiliated Hospital of Third Military Medical University, Chongqing, People’s Republic of China

    Rong Zhang

Authors
  1. Lai-chun Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ming-bao Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shi-wen Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gui-sheng Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gui-sheng Qian.

Additional information

Lai-chun Lu and Rong Zhang have contributed equally to the manuscript.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lu, Lc., Zhang, R., Song, Mb. et al. Optimization of Extraction and Purification of Arctiin from Fructus arctii and Its Protection Against Glucose-Induced Rat Aortic Endothelial Cell Injury. Cell Biochem Biophys 69, 93–101 (2014). https://doi.org/10.1007/s12013-013-9775-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12013-013-9775-5

Keywords

Search

Navigation