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Cell Biochemistry and Biophysics

, Volume 68, Issue 3, pp 497–509 | Cite as

Delphinidin Activates NFAT and Induces IL-2 Production Through SOCE in T Cells

  • Evelyn Jara
  • María A. Hidalgo
  • Juan L. Hancke
  • Alejandra I. Hidalgo
  • Sebastian Brauchi
  • Luisa Nuñez
  • Carlos Villalobos
  • Rafael A. BurgosEmail author
Original Paper

Abstract

Delphinidin is an anthocyanidin that possesses antioxidant and anti-inflammatory effects; however, some reports suggest that delphinidin has pro-inflammatory properties. For this reason, we assessed the effect of delphinidin on cytokine production in T cells. We demonstrated that delphinidin increased the cytosolic-free Ca2+ concentration by releasing Ca2+ from intracellular stores and increasing Ca2+ entry. The putative Ca2+ release activated Ca2+ (CRAC) channel inhibitors BTP2 and gadolinium reduced the calcium entry stimulated by the anthocyanidin. Delphinidin induced nuclear factor of activated T cells (NFAT) translocation and NFAT-Luc activity in Jurkat cells and was dependent on the CRAC channel and calcineurin pathway. Delphinidin increased the mRNA expression and production of IL-2 in Jurkat cells and was inhibited by BTP2 and cyclosporine A. Using peripheral blood lymphocytes, we demonstrated that delphinidin increased the production of IL-2 and IFN-γ and was inhibited by BTP2. Taken together, our results suggest that delphinidin exerts immunostimulatory effects on T cells by increasing cytokine production through CRAC channel and NFAT activation.

Keywords

Delphinidin Calcium T cells NFAT Cytokines 

Abbreviations

SOCE

Store-operated calcium entry

BTP2

N-(4-[3,5-bis(trifluoromethyl)-1H-pyrazol-1-yl]phenyl)-4-methyl-1,2,3-thiadiazole-5-carboxamide

CRAC

Ca2+ release-activated Ca2+

Notes

Acknowledgments

This work was supported by Grants from Consorcio de Tecnología e Innovación para la Salud CTI-Salud (CTE-06), Chile (CONICYT 21090900 and CONICYT AT-24100037).

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Evelyn Jara
    • 1
  • María A. Hidalgo
    • 1
  • Juan L. Hancke
    • 1
  • Alejandra I. Hidalgo
    • 1
  • Sebastian Brauchi
    • 2
  • Luisa Nuñez
    • 3
  • Carlos Villalobos
    • 3
  • Rafael A. Burgos
    • 1
    Email author
  1. 1.Institute of Pharmacology and MorphophysiologyUniversidad Austral de ChileValdiviaChile
  2. 2.Institute of Physiology, Faculty of MedicineUniversidad Austral de ChileValdiviaChile
  3. 3.Spanish Research Council, Institute of Molecular Biology and Genetics University of ValladolidValladolidSpain

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