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Expression and Functional Significance of Ezrin in Human Brain Astrocytoma

  • Translational Biomedical Research
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Abstract

Ezrin is overexpressed in a variety of neoplastic cells and is involved in the later stages of tumor progression and metastasis. The present study investigated the expression and functional significance of ezrin in human brain astrocytoma. Ezrin expression was examined in specimens from healthy human brains (10 autopsies) or human astrocytoma (107 cases) by immunohistochemistry. All healthy specimens were negative for ezrin expression, while this expression was positive in a great majority of human astrocytoma tissues (96/107; 89.7 %; p < 0.05 vs. healthy). Ezrin expression was positively correlated with tumor grade (r = 0.551, p < 0.01). Analysis of clinicopathologic data revealed that the post-operation disease-free survival times were significantly (p < 0.001) different between those with a strong positive ezrin expression and those with a weak or negative expression. Specifically, median DFS in patients with a strongly positive ezrin expression was 13 months (range 2–46 months), while it was significantly (p < 0.001) longer in patients with weakly positive or negative expression (median of 28 months, range 6–56 months). In conclusion, there is a strong association between ezrin expression and increased malignancy in astrocytoma. Thus, enhanced ezrin expression may play an important role in the development of astrocytoma. Our results further indicate that ezrin may be useful for grading of astrocytoma and as a molecular marker for the prognosis.

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References

  1. Schneider, T., Mawrin, C., Scherlach, C., Skalej, M., & Firsching, R. (2010). Gliomas in adults. Deutsches Aerzteblatt International, 107, 799–807.

    Google Scholar 

  2. Jiang, T., Tang, G. F., Lin, Y., Peng, X. X., Zhang, X., Zhai, X. W., et al. (2011). Prevalence estimates for primary brain tumors in China: A multi-center cross-sectional study. Chinese Medical Journal, 124, 2578–2583.

    PubMed  Google Scholar 

  3. Wong, E. T., & Yamaguchi, N. H. (2011). Treatment advances for glioblastoma. Expert Review of Neurotherapeutics, 11, 1343–1345.

    Article  PubMed  Google Scholar 

  4. Grossman, S. A., Ye, X., Piantadosi, S., Desideri, S., Nabors, L. B., Rosenfeld, M., et al. (2010). Survival of patients with newly diagnosed glioblastoma treated with radiation and temozolomide in research studies in the United States. Clinical Cancer Research, 16, 2443–2449.

    Article  PubMed  CAS  Google Scholar 

  5. Ren, L., Hong, S. H., Cassavaugh, J., Osborne, T., Chou, A. J., Kim, S. Y., et al. (2009). The actin-cytoskeleton linker protein ezrin is regulated during osteosarcoma metastasis by PKC. Oncogene, 28, 792–802.

    Article  PubMed  CAS  Google Scholar 

  6. Kim, E. K., Park, J. M., Lim, S., Choi, J. W., Kim, H. S., Seok, H., et al. (2011). Activation of AMP-activated protein kinase is essential for lysophosphatidic acid-induced cell migration in ovarian cancer cells. Journal of Biological Chemistry, 286, 24036–24045.

    Article  PubMed  CAS  Google Scholar 

  7. Lorentzen, A., Bamber, J., Sadok, A., Elson-Schwab, I., & Marshall, C. J. (2011). An ezrin-rich, rigid uropod-like structure directs movement of amoeboid blebbing cells. Journal of Cell Science, 124, 1256–1267.

    Article  PubMed  CAS  Google Scholar 

  8. Hertweck, M. K., Erdfelder, F., & Kreuzer, K. A. (2011). CD44 in hematological neoplasias. Annals of Hematology, 90, 493–508.

    Article  PubMed  CAS  Google Scholar 

  9. Geiger, K. D., Stoldt, P., Schlote, W., & Derouiche, A. (2000). Ezrin immunoreactivity is associated with increasing malignancy of astrocytic tumors but is absent in oligodendrogliomas. American Journal of Pathology, 157, 1785–1793.

    Article  PubMed  CAS  Google Scholar 

  10. Tynninen, O., Carpen, O., Jaaskelainen, J., Paavonen, T., & Paetau, A. (2004). Ezrin expression in tissue microarray of primary and recurrent gliomas. Neuropathology and Applied Neurobiology, 30, 472–477.

    Article  PubMed  CAS  Google Scholar 

  11. Louis, D. N., Ohgaki, H., Wiestler, O. D., Cavenee, W. K., Burger, P. C., Jouvet, A., et al. (2007). The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathologica, 114, 97–109.

    Article  PubMed  Google Scholar 

  12. Mathew, J., Hines, J. E., Obafunwa, J. O., Burr, A. W., Toole, K., & Burt, A. D. (1996). CD44 is expressed in hepatocellular carcinomas showing vascular invasion. The Journal of Pathology, 179, 74–79.

    Article  PubMed  CAS  Google Scholar 

  13. Louvet-Vallee, S. (2000). ERM proteins: From cellular architecture to cell signaling. Biology of the Cell, 92, 305–316.

    Article  PubMed  CAS  Google Scholar 

  14. Bretscher, A., Edwards, K., & Fehon, R. G. (2002). ERM proteins and merlin: Integrators at the cell cortex. Nature Reviews Molecular Cell Biology, 3, 586–599.

    Article  PubMed  CAS  Google Scholar 

  15. Cassano, N., Loconsole, F., Amoruso, A., Coviello, C., Filieri, M., Filotico, R., et al. (2004). Infliximab monotherapy for refractory psoriasis: Preliminary results. International Journal of Immunopathology and Pharmacology, 17, 373–380.

    PubMed  CAS  Google Scholar 

  16. Gronholm, M., Teesalu, T., Tyynela, J., Piltti, K., Bohling, T., Wartiovaara, K., et al. (2005). Characterization of the NF2 protein merlin and the ERM protein ezrin in human, rat, and mouse central nervous system. Molecular and Cellular Neuroscience, 28, 683–693.

    Article  PubMed  Google Scholar 

  17. Pang, S. T., Fang, X., Valdman, A., Norstedt, G., Pousette, A., Egevad, L., et al. (2004). Expression of ezrin in prostatic intraepithelial neoplasia. Urology, 63, 609–612.

    Article  PubMed  Google Scholar 

  18. Ohtani, K., Sakamoto, H., Rutherford, T., Chen, Z., Kikuchi, A., Yamamoto, T., et al. (2002). Ezrin, a membrane-cytoskeletal linking protein, is highly expressed in atypical endometrial hyperplasia and uterine endometrioid adenocarcinoma. Cancer Letters, 179, 79–86.

    Article  PubMed  CAS  Google Scholar 

  19. Khanna, C., Wan, X., Bose, S., Cassaday, R., Olomu, O., Mendoza, A., et al. (2004). The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nature Medicine, 10, 182–186.

    Article  PubMed  CAS  Google Scholar 

  20. Khanna, C., Khan, J., Nguyen, P., Prehn, J., Caylor, J., Yeung, C., et al. (2001). Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Research, 61, 3750–3759.

    PubMed  CAS  Google Scholar 

  21. Chai, L., Sun, K., Guo, L., Zhang, H., & Lu, S. (2007). Expression of ezrin and CD44–v6 in human esophageal squamous cell carcinoma and its clinical significance. Chinese Journal of Clinical Oncology, 29, 685–688. In Chinese.

    CAS  Google Scholar 

  22. Elzagheid, A., Korkeila, E., Bendardaf, R., Buhmeida, A., Heikkila, S., Vaheri, A., et al. (2008). Intense cytoplasmic ezrin immunoreactivity predicts poor survival in colorectal cancer. Human Pathology, 39, 1737–1743.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Neurosurgery, Yijishan Hospital, Wannan Medical College, 2 West Zheshan Road, Wuhu, 241001, People’s Republic of China

    Jie Mao, Xian-rui Yuan, Shan-shui Xu, Xiao-chun Jiang & Xin-tong Zhao

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  1. Jie Mao
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  2. Xian-rui Yuan
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  3. Shan-shui Xu
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  4. Xiao-chun Jiang
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  5. Xin-tong Zhao
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Correspondence to Jie Mao.

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Mao, J., Yuan, Xr., Xu, Ss. et al. Expression and Functional Significance of Ezrin in Human Brain Astrocytoma. Cell Biochem Biophys 67, 1507–1511 (2013). https://doi.org/10.1007/s12013-013-9653-1

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