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Association with Leptin Gene c.-2548 G>A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients

Cell Biochemistry and Biophysics volume 65, pages 243–247 (2013)Cite this article

Abstract

Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G>A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G>A polymorphism were detected using polymerase chain reaction–restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G>A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %) when compared with controls (14.6, 12.5, and 27.1 %; p = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels (p < 0.001) and body mass index (p < 0.0001). All these consequences showed that LEP -2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population.

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References

  1. Haslam, D. W., & James, W. P. (2005). Obesity. Lancet, 366, 1197–1209.

    PubMed  Article  Google Scholar 

  2. Bagriacik, N., Onat, H., Ilhan, B., Tarakci, T., Oşar, Z., Ozyazar, M., et al. (2009). Obesity profile in Turkey. International Journal of Diabetes and Metabolism, 17, 5–8.

    Google Scholar 

  3. Cummings, D. E., & Schwartz, M. W. (2003). Genetics and pathophysiology of human obesity. Annual Review of Medicine, 54, 453–471.

    PubMed  Article  CAS  Google Scholar 

  4. Farooqi, S., & O’Rahilly, S. (2006). Genetics of obesity in humans. Endocrine Reviews, 27, 710–718.

    PubMed  CAS  Google Scholar 

  5. Williams, M. J., Almen, M. S., Fredriksson, S., & Schioth, H. B. (2012). What model organism and interactomics can reveal about the genetics of human obesity. Cellular and Molecular Life Sciences,. doi:10.1007/s00018-012-1022-5.

    PubMed  Google Scholar 

  6. Houseknecht, K. L., Baile, C. A., Matteri, R. L., & Spurlock, M. E. (1998). The biology of leptin: A review. Journal of Animal Science, 76, 1405–1420.

    PubMed  CAS  Google Scholar 

  7. Houseknecht, K. L., & Portocarrero, C. P. (1998). Leptin and its receptors: Regulators of whole-body energy homeostasis. Domestic Animal Endocrinology, 15, 457–475.

    PubMed  Article  CAS  Google Scholar 

  8. Caro, J. F., Sinha, M. K., Kolaczynski, J. W., Zhang, P. L., & Considine, R. V. (1996). Leptin: The tale of an obesity gene. Diabetes, 45, 1455–1462.

    PubMed  CAS  Google Scholar 

  9. Spiegelman, B. M., & Flier, J. S. (1996). Adipogenesis and obesity: Rounding out the big picture. Cell, 87, 377–389.

    PubMed  Article  CAS  Google Scholar 

  10. Jequier, E. (2002). Leptin signaling, adiposity, and energy balance. Annals of the New York Academy of Sciences, 967, 379–388.

    PubMed  Article  CAS  Google Scholar 

  11. Considine, R. V. (2005). Human leptin: An adipocyte hormone with weight-regulatory and endocrine functions. Seminars in Vascular Medicine, 5, 15–24.

    PubMed  Article  Google Scholar 

  12. Fruhbeck, G. (2006). Intracellular signalling pathways activated by leptin. Biochemical Journal, 393, 7–20.

    PubMed  Article  CAS  Google Scholar 

  13. Isse, N., Ogawa, Y., Tamura, N., Masuzaki, H., Mori, K., Okazaki, T., et al. (1995). Structural organization and chromosomal assignment of the human obese subjects. Journal of Biological Chemistry, 270, 27728–27733.

    PubMed  Article  CAS  Google Scholar 

  14. Gong, D. W., Bi, S., Pratley, R. E., & Weintraub, B. D. (1996). Genomic structure and promoter analysis of the human obese gene. Journal of Biological Chemistry, 271, 3971–3974.

    PubMed  Article  CAS  Google Scholar 

  15. Paracchini, V., Pedotti, P., & Taioli, E. (2005). Genetics of leptin and obesity: A huge review. American Journal of Epidemiology, 162, 101–104.

    PubMed  Article  Google Scholar 

  16. Rankinen, T., Zuberi, A., Chagnon, Y. C., Weisnagel, S. J., Argyropoulos, G., Walts, B., et al. (2006). The human obesity gene map: The 2005 update. Obesity, 14, 529–644.

    PubMed  Article  Google Scholar 

  17. Mammes, O., Betoulle, D., Aubert, R., Herbeth, B., Siest, G., & Fumeron, F. (2000). Association of the C.-2548 G>A polymorphism in the 5′ region of the LEP gene with overweight. Annals of Human Genetics, 64, 391–394.

    PubMed  Article  CAS  Google Scholar 

  18. Le Stunff, C., Le Bihan, C., Schork, N. J., & Bougneres, P. (2000). A common promoter variant of the leptin gene is associated with changes in the relationship between serum leptin and fat mass in obese girls. Diabetes, 49, 2196–2200.

    PubMed  Article  Google Scholar 

  19. Hoffstedt, J., Eriksson, P., Mottagui-Tabar, S., & Arner, P. (2002). A polymorphism in the leptin promoter region (-2548 G/A) influences gene expression and adipose tissue secretion of leptin. Hormone and Metabolic Research, 43, 355–359.

    Article  Google Scholar 

  20. World Health Organization. (2000). Obesity: Preventing and managing the global epidemic. Report of a WHO consultation. World Health Organization Technical Report Series, 894, 1–253.

    Google Scholar 

  21. Friedewald, W. T., Levy, R. I., & Fredericson, D. S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in serum, without use of the preparative ultracentrifuge. Clinical Chemistry, 18, 499–502.

    PubMed  CAS  Google Scholar 

  22. Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16, 1215.

    PubMed  Article  CAS  Google Scholar 

  23. Yiannakouris, N., Relistas, L., Yannakoulia, M., Mungal, K., & Mantzoros, C. S. (2003). The 2548G/A polymorphism in the human leptin gene promoter region is associated with serum free leptin levels: Interaction with adiposity and gender in health subjects. Hormones, 2, 229–236.

    PubMed  Google Scholar 

  24. Mammes, O., Betoulle, D., Aubert, R., Giraud, V., Tuzet, S., Petiet, A., et al. (1998). Novel polymorphisms in the 5′ region of the LEP gene: Association with leptin levels and response to low-calorie diet in human obesity. Diabetes, 47, 487–489.

    PubMed  Article  CAS  Google Scholar 

  25. Hinuy, H. M., Hitara, M. H., Forti, N., Diament, J., Sampaio, M. F., Armaganijan, D., et al. (2008). Leptin G-2548A promoter polymorphism is associated with increased serum leptin and BMI in Brazilian women. Arquivos Brasileiros de Endocrinologia & Metabologia, 52, 611–616.

    Article  Google Scholar 

  26. Ren, W., Zhang, S. H., Wu, J., & Ni, Y. X. (2004). Polymorphism of the leptin gene promoter in pedigrees of type 2 diabetes mellitus in Chongqing, China. Chinese Medical Journal, 117, 558–561.

    PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by a grant from the Mustafa Kemal University Scientific Research Projects Unit, Hatay, Turkey (Project Nr. MKUBAP1002Y0102).

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Affiliations

  1. Department of Medical Biology and Genetics, Faculty of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey

    Mustafa Celik & Ramazan Gunesacar

  2. Vocational Higher School of Health Services, Mardin Artuklu University, 47100, Mardin, Turkey

    Deniz Say Sahin & Cemil Demir

  3. Department of Physiology, Faculty of Medicine, Mustafa Kemal University, 31100, Hatay, Turkey

    Cemil Tumer

  4. Department of Internal Medicine, Faculty of Medicine, Mustafa Kemal University, 31100, Hatay, Turkey

    M. Murat Celik & Edip Ucar

Authors
  1. Deniz Say Sahin
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  2. Cemil Tumer
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  3. Cemil Demir
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  4. M. Murat Celik
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  5. Mustafa Celik
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  6. Edip Ucar
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  7. Ramazan Gunesacar
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Correspondence to Ramazan Gunesacar.

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Sahin, D.S., Tumer, C., Demir, C. et al. Association with Leptin Gene c.-2548 G>A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients. Cell Biochem Biophys 65, 243–247 (2013). https://doi.org/10.1007/s12013-012-9427-1

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  • DOI: https://doi.org/10.1007/s12013-012-9427-1

Keywords

  • Leptin
  • c.-2548 G>A
  • Polymorphism
  • Obesity
  • Body mass index