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Resuscitative Effect of Hyperoxia Fluid on High-Altitude Hemorrhagic Shock in Rats and Antishock Mechanisms

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Abstract

Pathophysiological characteristics of hemorrhagic shock at high altitude are different from that at plain which involve severe injury, high mortality, difficult treatment and compromised liquid tolerance. High-altitude pulmonary/cerebral edema and multiple-organ dysfunction render the conventional treatment ineffective. Herein, we evaluated the resuscitation effects of hyperoxia solution on high-altitude hemorrhagic shock in rats. For this purpose, a rat model of high-altitude (3,658 m) hemorrhagic shock was established on the plateau and hyperoxia solution (4 ml/kg) was infused through external jugular vein for resuscitation at 60 min post-hemorrhage. Blood pressure, blood gas, left and right ventricular pressure, lung and brain water content, survival time, survival rate at 2 h, levels of inflammatory cytokines and free oxygen radicals in blood and tissue were determined. After resuscitation with hyperoxia solution, blood pressure, arterial oxygen partial pressure, left and right ventricular systolic pressure, ±dp/dt max, survival time and rate were significantly increased. Lung and brain water content were unchanged, malondialdehyde activity in lung, brain and plasma and levels of TNF-α, IL-1, IL-6, and endothelin were significantly decreased. Besides, CGRP was elevated with reduced injury and improved lung and kidney functions. Concludingly, resuscitation with hyperoxia solution is feasible and more effective than other classical liquids, making it the first choice of treatment for high-altitude hemorrhagic shock.

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Acknowledgments

The authors thank the personnel from Research Center of Mountain Sickness and Experimental Animal Center, General Hospital of Tibetan Military Area of the Chinese PLA for their collaboration. This work was supported by the Chinese PLA under its 11th 5-Year Plan for Scientific and Technological Research (Grant # 2006Z030), the National Science Foundation of China (Grant # 30900715), and the National Science and Technology Ministry (Grant # 2009BAI85B03).

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Authors and Affiliations

  1. Department of High Altitude Diseases, College of High Altitude Military Medicine, 3rd Military Medical University, Chongqing, 400038, China

    Qiquan Zhou & Yongjun Luo

  2. Key Laboratory of High Altitude Medicine, 3rd Military Medical University, Ministry of Education, Chongqing, 400038, China

    Qiquan Zhou, Yongjun Luo, Fuyu Liu & Yuqi Gao

  3. Research Center of Mountain Sickness, General Hospital of Tibetan Military Area, Lhasa, 850007, China

    Yi He, Bihai Zheng, Dingzhou Yang & Suzhi Li

  4. Department-2 Research, Institute of Field Battle Surgery Research, 3rd Military Medical University, Chongqing, 400042, China

    Liangming Liu

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  1. Qiquan Zhou
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Correspondence to Qiquan Zhou.

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Zhou, Q., Luo, Y., Liu, F. et al. Resuscitative Effect of Hyperoxia Fluid on High-Altitude Hemorrhagic Shock in Rats and Antishock Mechanisms. Cell Biochem Biophys 62, 343–352 (2012). https://doi.org/10.1007/s12013-011-9316-z

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