Skip to main content

Advertisement

SpringerLink
Log in

Survivin siRNA Inhibits Gastric Cancer in Nude Mice

  • Original Paper
  • Published:
Cell Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

The objective of the study was to evaluate the expression of survivin, cell proliferation, and apoptosis in survivin-specific siRNA-transfected human gastric cancer cell line MGC-803. For this purpose, the target gene fragments were cloned into pSilencer3.1-Hl neo vector. Recombinant eukaryotic expression vector, pSilencer3.1-SVV was successfully constructed and then the recombinant vector was transfected into gastric cancer MGC-803 cells. The mRNA expression of survivin was determined by reverse-transcriptase polymerase chain reaction (RT-PCR). Survivin protein expression was detected by Western blot. Cell cycle distribution and apoptosis were determined by flow cytometry. Our data regarding RT-PCR and Western blot showed that pSilencer3.1-SVV vector could knockdown the expression of survivin mRNA and protein. In contrast with the control group, the apoptotic index of MGC-803 cells increased remarkably. Survivin-specific siRNA caused cells accumulation in the G2/M phase and the number of cells in the G0/G1 phase decreased after transfection. It was, therefore, concluded that the siRNA targeting survivin gene could inhibit the proliferation of gastric cancer cells and induce apoptosis. The use of survivin siRNA may provide a novel approach for gene therapy of gastric cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Maolin, J. (2000). Neoadjuvant chemotherapy treatment in the function of gastric cancer. Journal of Surgical Concepts and Practices, 5, 140–142.

    Google Scholar 

  2. Ambrosini, G., Adida, C., & Altieri, D. C. (1997). A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Natural Medicines, 3, 917–921.

    Article  CAS  Google Scholar 

  3. Altieri, D. C., & Marchisio, P. C. (1999). Survivin apoptosis: An interloper between cell death and cell proliferation in cancer. Laboratory Investigation, 79, 1327–1333.

    PubMed  CAS  Google Scholar 

  4. Tsuburaya, A., Noguchi, Y., Yoshikawa, T., Saito, A., Doi, C., Okamoto, T., et al. (2002). An anti-apoptosis gene, survivin and telomerase expression in gastric cancer. Hepato-Gastroenterology, 46, 1150–1152.

    Google Scholar 

  5. Li, Y. H., Wang, C., Meng, K., Chen, L. B., & Zhou, X. J. (2004). Influence of survivin and caspase-3 on cell apoptosis and prognosis in gastric carcinoma. World Journal of Gastroenterology, 10, 1984–1988.

    PubMed  CAS  Google Scholar 

  6. Xiaoyun, L., & Nanzhi, L. (2004). The correlation of Survivin expression in gastric cancer tissue and the expression of caspase-3 and p53. Journal of Huazhong University of Health Sciences and Technology, 33, 724–725.

    Google Scholar 

  7. Conway, E. M., Pollefeyt, S., Cornelissen, J., DeBaere, I., Steiner-Mosonyi, M., Ong, K., et al. (2000). Three differentially expressed Survivin cDNA variants encode proteins with distinct antiapoptotic functions. Blood, 95, 1435–1442.

    PubMed  CAS  Google Scholar 

  8. Ambrosini, G., Adida, C., Sirugo, G., & Altieri, D. C. (1998). Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting. The Journal of Biological Chemistry, 273, 11177–11182.

    Article  PubMed  CAS  Google Scholar 

  9. Wall, N. R., & Shi, Y. (2003). Small RNA: Can RNA interference be exploited for therapy? Lancet, 362, 1401–1403.

    Article  PubMed  CAS  Google Scholar 

  10. Chi, J. T., Chang, H. Y., Wang, N. N., Chang, D. S., Dunphy, N., & Brown, P. O. (2003). Genome wide view of gene silencing by small interfering RNAs. Proceedings of the National Academy of Sciences of the United States of America, 100, 6343–6346.

    Article  PubMed  CAS  Google Scholar 

  11. Brummelkamp, T. R., Bernards, R., & Agami, R. (2002). Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell, 2, 243–247.

    Article  PubMed  CAS  Google Scholar 

  12. Li, F., Ambrosini, G., Chu, E. Y., Plescia, J., Tognin, S., Marchisio, P. C., et al. (1998). Control of apoptosis and mitotic spindle checkpoint by survivin. Nature, 396, 580–584.

    Article  PubMed  CAS  Google Scholar 

  13. Grossman, D., Kim, P. J., Blanc-Brude, O. P., Brash, D. E., Tognin, S., Marchisio, P. C., et al. (2001). Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53. The Journal of Clinical Investigation, 108, 991–999.

    PubMed  CAS  Google Scholar 

  14. Ikeguchi, M., Ueda, T., Sakatani, T., Hirooka, Y., & Kaibara, N. (2002). Expression of survivin messenger RNA correlates with poor prognosis in patients with hepatocellular carcinoma. Diagnostic Molecular Pathology, 11, 33–40.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors appreciate assistance from personnel of the Central Lab of Yijishan Hospital Wannan Medical College, China. This study was supported by a research grant (WK200815F) from the Wannan Medical College, Anhui Wuhu, China.

Author information

Authors and Affiliations

  1. Department of Medical Oncology, Yijishan Hospital Wannan Medical College, Anhui Wuhu, 241000, China

    Zhao Wenying, Ji Zhaoning, Yang Zhimin, Chen Dongyun & Sheng Lili

Authors
  1. Zhao Wenying
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ji Zhaoning
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yang Zhimin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chen Dongyun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sheng Lili
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhao Wenying.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wenying, Z., Zhaoning, J., Zhimin, Y. et al. Survivin siRNA Inhibits Gastric Cancer in Nude Mice. Cell Biochem Biophys 62, 337–341 (2012). https://doi.org/10.1007/s12013-011-9315-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12013-011-9315-0

Keywords

Search

Navigation