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Cell Biochemistry and Biophysics

, Volume 67, Issue 2, pp 297–304 | Cite as

Piperine Attenuates Cardiovascular, Liver and Metabolic Changes in High Carbohydrate, High Fat-Fed Rats

  • Vishal Diwan
  • Hemant Poudyal
  • Lindsay Brown
Original Paper

Abstract

Black pepper is used worldwide to enhance food flavor. We investigated dietary supplementation with piperine, the active principle of black pepper, to high carbohydrate, high fat (HCHF) diet-fed rats as a model of human metabolic syndrome. Rats were fed with either HCHF diet (carbohydrate, 52%; fat, 24%; 25% fructose in drinking water) or cornstarch (CS) diet for a total of 16 weeks. Diets of the treatment groups (CS + piperine and HCHF + piperine) were supplemented with piperine for the last 8 weeks of this protocol. After 16 weeks, rats fed with HCHF diet developed hypertension, elevated oxidative stress and inflammation-induced cardiac changes (infiltration of inflammatory cells in heart, increase in count and degranulation of mast cells in heart, cardiac fibrosis and increase in ventricular stiffness), reduced responsiveness of aortic rings, impaired glucose tolerance, abdominal obesity together with liver fibrosis, fat deposition and increased plasma liver enzymes. Supplementation with piperine (375 mg/kg food; approximately 30 mg/kg/day) in HCHF-fed rats normalized blood pressure, improved glucose tolerance and reactivity of aortic rings, reduced plasma parameters of oxidative stress and inflammation, attenuated cardiac and hepatic inflammatory cell infiltration and fibrosis and improved liver function. These changes clearly suggest that piperine reduces symptoms of human metabolic syndrome in HCHF-fed rats by reducing inflammation and oxidative stress.

Keywords

Piperine Metabolic syndrome Rats 

References

  1. 1.
    Iyer, A., Panchal, S., Poudyal, H., & Brown, L. (2009). Potential health benefits of Indian spices in the symptoms of the metabolic syndrome: a review. Indian Journal of Biochemistry and Biophysics, 46, 467–481.PubMedGoogle Scholar
  2. 2.
    Ee, G. C., Lim, C. M., Lim, C. K., Rahmani, M., Shaari, K., & Bong, C. F. (2009). Alkaloids from Piper sarmentosum and Piper nigrum. Natural Product Research, 23, 1416–1423.PubMedCrossRefGoogle Scholar
  3. 3.
    Taqvi, S. I., Shah, A. J., & Gilani, A. H. (2008). Blood pressure lowering and vasomodulator effects of piperine. Journal of Cardiovascular Pharmacology, 52, 452–458.PubMedCrossRefGoogle Scholar
  4. 4.
    Bang, J. S., Ohda, H., Choi, H. M., et al. (2009). Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1beta-stimulated fibroblast-like synoviocytes and in rat arthritis models. Arthritis Res Ther, 11, R49.PubMedCrossRefGoogle Scholar
  5. 5.
    Vijayakumar, R. S., & Nalini, N. (2006). Efficacy of piperine, an alkaloidal constituent from Piper nigrum on erythrocyte antioxidant status in high fat diet and antithyroid drug induced hyperlipidemic rats. Cell Biochemistry and Function, 24, 491–498.PubMedCrossRefGoogle Scholar
  6. 6.
    Vijayakumar, R. S., Surya, D., & Nalini, N. (2004). Antioxidant efficacy of black pepper (Piper nigrum L.) and piperine in rats with high fat diet induced oxidative stress. Redox Report, 9, 105–110.PubMedCrossRefGoogle Scholar
  7. 7.
    Khajuria, A., Thusu, N., & Zutshi, U. (2002). Piperine modulates permeability characteristics of intestine by inducing alterations in membrane dynamics: influence on brush border membrane fluidity, ultrastructure and enzyme kinetics. Phytomedicine, 9, 224–231.PubMedCrossRefGoogle Scholar
  8. 8.
    Panchal S. K., Brown L. (2011). Rodent models for metabolic syndrome research. Journal of Biomedicine and Biotechnology. doi: 10.1155/2011/351982.
  9. 9.
    Panchal, S., Poudyal, H., Iyer, A., et al. (2011). High carbohydrate-high fat diet-induced metabolic syndrome and cardiovascular remodeling in rats. Journal of Cardiovascular Pharmacology, 57, 611–624.PubMedCrossRefGoogle Scholar
  10. 10.
    Poudyal, H., Campbell, F., & Brown, L. (2010). Olive leaf extract attenuates cardiac, hepatic, and metabolic changes in high carbohydrate-, high fat-fed rats. Journal of Nutrition, 140, 946–953.PubMedCrossRefGoogle Scholar
  11. 11.
    Poudyal, H., Panchal, S., & Brown, L. (2010). Comparison of purple carrot juice and beta-carotene in a high-carbohydrate, high-fat diet-fed rat model of the metabolic syndrome. British Journal of Nutrition, 104, 1322–1332.PubMedCrossRefGoogle Scholar
  12. 12.
    Brown, L., Fenning, A., Chan, V., et al. (2002). Echocardiographic assessment of cardiac structure and function in rats. Heart Lung and Circulation, 11, 167–173.CrossRefGoogle Scholar
  13. 13.
    Litwin, S. E., Katz, S. E., Morgan, J. P., & Douglas, P. S. (1994). Serial echocardiographic assessment of left ventricular geometry and function after large myocardial infarction in the rat. Circulation, 89, 345–354.PubMedCrossRefGoogle Scholar
  14. 14.
    Fenning, A., Harrison, G., Rose’meyer, R., Hoey, A., & Brown, L. (2005). l-Arginine attenuates cardiovascular impairment in DOCA-salt hypertensive rats. American Journal of Physiology Heart and Circulatory Physiology, 289, H1408–H1416.PubMedCrossRefGoogle Scholar
  15. 15.
    Rice-Evans, C., & Miller, N. J. (1994). Total antioxidant status in plasma and body fluids. Methods in Enzymology, 234, 279–293.PubMedCrossRefGoogle Scholar
  16. 16.
    Gupta, S. K., Bansal, P., Bhardwaj, R. K., & Velpandian, T. (2000). Comparative anti-nociceptive, anti-inflammatory and toxicity profile of nimesulide vs nimesulide and piperine combination. Pharmacological Research, 41, 657–662.PubMedCrossRefGoogle Scholar
  17. 17.
    D’Hooge, R., Pei, Y. Q., Raes, A., Lebrun, P., van Bogaert, P. P., & de Deyn, P. P. (1996). Anticonvulsant activity of piperine on seizures induced by excitatory amino acid receptor agonists. Arzneimittelforschung, 46, 557–560.PubMedGoogle Scholar
  18. 18.
    Bai, Y. F., & Xu, H. (2000). Protective action of piperine against experimental gastric ulcer. Acta Pharmacologica Sinica, 21, 357–359.PubMedGoogle Scholar
  19. 19.
    Lee, S. A., Hong, S. S., Han, X. H., et al. (2005). Piperine from the fruits of Piper longum with inhibitory effect on monoamine oxidase and antidepressant-like activity. Chemical and Pharmaceutical Bulletin, 53, 832–835.PubMedCrossRefGoogle Scholar
  20. 20.
    Selvendiran, K., Singh, J. P., Krishnan, K. B., & Sakthisekaran, D. (2003). Cytoprotective effect of piperine against benzo[a]pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice. Fitoterapia, 74, 109–115.PubMedCrossRefGoogle Scholar
  21. 21.
    Shah, S. S., Shah, G. B., Singh, S. D., Gohil, P. V., Chauhan, K., Shah, K. A., et al. (2011). Effect of piperine in the regulation of obesity-induced dyslipidemia in high-fat diet rats. Indian Journal of Pharmacology, 43, 296–299.PubMedCrossRefGoogle Scholar
  22. 22.
    Pepine, C. J. (1998). Clinical implications of endothelial dysfunction. Clinical Cardiology, 21, 795–799.PubMedCrossRefGoogle Scholar
  23. 23.
    Brunner, H., Cockcroft, J. R., Deanfield, J., et al. (2005). Endothelial function and dysfunction. Part II: association with cardiovascular risk factors and diseases. A statement by the Working Group on Endothelins and Endothelial Factors of the European Society of Hypertension. Journal of Hypertension, 23, 233–246.PubMedCrossRefGoogle Scholar
  24. 24.
    Tziomalos, K., Athyros, V. G., Karagiannis, A., & Mikhailidis, D. P. (2010). Endothelial dysfunction in metabolic syndrome: prevalence, pathogenesis and management. Nutrition, Metabolism and Cardiovascular Diseases, 20, 140–146.PubMedCrossRefGoogle Scholar
  25. 25.
    Aktoz, M., Erdogan, O., & Altun, A. (2007). Electrocardiographic prediction of left ventricular geometric patterns in patients with essential hypertension. International Journal of Cardiology, 120, 344–350.PubMedCrossRefGoogle Scholar
  26. 26.
    Savoia, C., & Schiffrin, E. L. (2006). Inflammation in hypertension. Current Opinion in Nephrology and Hypertension, 15, 152–158.PubMedGoogle Scholar
  27. 27.
    Levick, S. P., McLarty, J. L., Murray, D. B., Freeman, R. M., Carver, W. E., & Brower, G. L. (2009). Cardiac mast cells mediate left ventricular fibrosis in the hypertensive rat heart. Hypertension, 53, 1041–1047.PubMedCrossRefGoogle Scholar
  28. 28.
    van Heerebeek, L., Somsen, A., & Paulus, W. J. (2009). The failing diabetic heart: focus on diastolic left ventricular dysfunction. Current Diabetes Reports, 9, 79–86.PubMedCrossRefGoogle Scholar
  29. 29.
    Klotz, S., Foronjy, R. F., Dickstein, M. L., et al. (2005). Mechanical unloading during left ventricular assist device support increases left ventricular collagen cross-linking and myocardial stiffness. Circulation, 112, 364–374.PubMedCrossRefGoogle Scholar
  30. 30.
    Harrison, D. G., & Gongora, M. C. (2009). Oxidative stress and hypertension. Medical Clinics of North America, 93, 621–635.PubMedCrossRefGoogle Scholar
  31. 31.
    Sowers, J. R., & Frohlich, E. D. (2004). Insulin and insulin resistance: impact on blood pressure and cardiovascular disease. Medical Clinics of North America, 88, 63–82.PubMedCrossRefGoogle Scholar
  32. 32.
    Patel, K., & Srinivasan, K. (2000). Influence of dietary spices and their active principles on pancreatic digestive enzymes in albino rats. Nahrung, 44, 42–46.CrossRefGoogle Scholar
  33. 33.
    Clark, J. M., Brancati, F. L., & Diehl, A. M. (2002). Nonalcoholic fatty liver disease. Gastroenterology, 122, 1649–1657.PubMedCrossRefGoogle Scholar
  34. 34.
    Chitturi, S., Wong, V. W., & Farrell, G. (2011). Nonalcoholic fatty liver in Asia: firmly entrenched and rapidly gaining ground. Journal of Gastroenterology and Hepatology, 26(Suppl 1), 163–172.PubMedCrossRefGoogle Scholar
  35. 35.
    Giannini, E. G., Testa, R., & Savarino, V. (2005). Liver enzyme alteration: a guide for clinicians. CMAJ, 172, 367–379.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.School of Biomedical SciencesThe University of QueenslandBrisbaneAustralia
  2. 2.Department of Biological and Physical SciencesUniversity of Southern QueenslandToowoombaAustralia

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