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Effects of Gekko Sulfated Polysaccharide–Protein Complex on the Defective Biorheological Characters of Dendritic Cells Under Tumor Microenvironment

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Abstract

We previously isolated a sulfated polysaccharide–protein complex from Gekko swinhonis Guenther, a traditional Chinese medicine, and have demonstrated its direct anti-cancer effect on human hepatocellular carcinoma cell line SMMC-7721. Here we investigated the effects of Gekko sulfated polysaccharide–protein complex (GSPP) on the defective biorheological characters of dendritic cells (DCs) under SMMC-7721 microenvironment. Our findings have shown that the biorheological properties of DCs were severely impaired by SMMC-7721 microenvironment, including decreased cell deformability, migration, and electrophoresis mobility, increased osmotic fragilities, and changed organizations of cytoskeletal proteins. We also found decreased secretion of interleukin (IL)-12 and increased secretion of IL-10 in DCs. However, supernatant collected from nonmalignant liver cells had no effect on these parameters. SMMC-7721 cells were treated with GSPP and the supernatant was used to culture DCs. We found that the defective biorheological parameters of DCs, except for osmotic fragility, were partially or completely improved. The secretion of IL-12 did not change as compared with that of DCs in SMMC-7721 microenvironment, but the secretion of IL-10 was resumed to the control level. Our results indicate that GSPP could partially restore the defective biorheological characteristics of DCs via modifying the tumor microenvironment and decreasing the secretion of IL-10 of DCs.

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Acknowledgment

This study was supported by National Natural Science Foundations of China (Grant Nos. 10572007 and 30770532).

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Authors and Affiliations

  1. Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China

    Dan Chen

  2. Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China

    Dan Chen, Weibo Ka, Dagong Sun, Weijuan Yao & Zongyao Wen

  3. Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Xiaolan Zhang

  4. Department of Urology, Peking University Third Hospital, Beijing, 100191, China

    Yuan Du

  5. Chengde Medical College, Chengde, 067000, Hebei Province, China

    Binbin Jia

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  1. Dan Chen
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Correspondence to Weijuan Yao or Zongyao Wen.

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Chen, D., Zhang, X., Du, Y. et al. Effects of Gekko Sulfated Polysaccharide–Protein Complex on the Defective Biorheological Characters of Dendritic Cells Under Tumor Microenvironment. Cell Biochem Biophys 62, 193–201 (2012). https://doi.org/10.1007/s12013-011-9282-5

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