Abstract
We evaluated the effect of Ganciclovir on murine cytomegalovirus (MCMV)-induced hearing impairment in a mouse model by studying modulations in auditory brainstem response (ABR) and pathological changes in the inner ear. MCMV infection was established via trans-brain. For this purpose, 24 BALB/c newborn mice were randomly and equally divided into control group (10 μl of sterile normal saline was injected); model group (10 μl of MCMV TCID50—104 IU/0.1 ml was injected); and interfered group (Trans-brain MCMV; Ganciclovir at the rate of 60 mg/kg was intraperitoneally injected). ABR audiometry was performed after 14 days. Acoustic vesicles were obtained for MCMV-PCR analysis and histopathological examination. Comparing ABR in model group against controls, incubation period of wave was lengthened (F = 9.151, P = 0.011–0.05) and wave amplitude was cut down (F = 5.095, P = 0.043–0.05). Comparing ABR in model group against interfered group, incubation period and amplitude of wave were F = 13.797 (P = 0.003–0.05) and F = 14.587 (P = 0.002–0.05), respectively. Cochlear histopathological changes in model group included thickening of vestibular membrane, lymphocytic infiltration, and fibrous degeneration of cochlear duct. In Ganciclovir interfered group, however, these pathologic changes were less significant as compared with those of model group. We, therefore, conclude that Ganciclovir treatment at the early stage of MCMV-induced hearing impairment inhibits the disease progression in infected mice.
Similar content being viewed by others
References
Li, Y. J., Zeng, Y., & Zhuang, S. Q. (2005). Clinical study of different methods on early diagnosis and monitoring of neonatal congenital cytomegalovirus infection. Chinese Journal of Perinatal Medicine, 8, 187–190.
Robe, F. P. (2005). Congenital cytomegalovirus infection and hearing loss. Herpes, 12, 50–56.
Fowler, K. B., & Boppan, S. B. (2006). Congenital cytomegalovirus (CMV) infection and hearing deficit. C1inical Viro1ogy, 35, 226–231.
Mo, W., Zhang, Y. Y., & Li, Q. Y. (2005). Hearing screening in infants with congenital cytomegalovirus infection. Journal of Zhejiang Medical Science University, 34, 358–360.
Dahle, A. J., Fowler, K. B., & Wright, J. D. (2000). Longitudinal investigation of hearing disorders in children with congenital cytomegalovirus. Journal of the American Academy of Audiology, 11, 283–290.
White, D. R., Choo, D. I., Stroup, G., & Schleiss, M. R. (2006). The effect of cidofovir on cytomegalovirus-induced hearing loss in a guinea pig model. Archives of Otolaryngology—Head and Neck Surgery, 132, 608–615.
Tang-Feldman, Y. J., Wojtowicz, A., Lochhead, G. R., Hale, M. A., Li, Y., & Pomeroy, C. (2006). Use of quantitative real-time PCR (qRT-PCR) to measure cytokine transcription and viral Load in murine cytomegalovirus infection. Virological Methods, 131, 122–129.
Liu, Q. M., Feng, B., & Lai, B. Y. (2006). The cause of hearing loss in 129 high-risk newborns. Journal of Clinical Otorhinolaryngology Head and Neck Surgery, 20, 325–326.
Hu, J. T., Xie, Z. D., & Chen, P. Y. (2007). Congenital cytomegalovirus infection and hearing deficit. Journal of Clinical Otorhinolaryngology Head and Neck Surgery, 10, 897–898.
Foweler, K. B., & Boppana, S. B. (2006). Congenital cytomegalovirus infection and hearing deficit. Journal of Clinical Virology, 35, 226–231.
Ross, S. A., Novak, Z. Z., Fowler, K. B., Nitin, A., Britt, W. J., & Boppana, S. B. (2009). Cytomegalovirus blood viral load and hearing loss in young children with congenital infection. The Pediatric Infectious Disease Journal, 28, 588–592.
Samileh, N., Ahmad, S., Mohammad, F., Framarz, M., Azardokht, T., & Jomeht, E. (2008). Role of cytomegalovirus in sensorineural hearing loss of children: A case-control study Tehran, Iran. International Journal of Pediatric Otorhinolaryngology, 72, 203–208.
Noyola, D. E., Demmler, G. J., Williamson, W. D., Griesser, C., Sellers, S., Llorente, A., et al. (2000). Cytomegalovirus urinary excretion and long term outcome in children with congenital cytomegalovirus infection. Pediatric Infectious Disease Journal, 19, 505–510.
Grosse, S. D., Ross, D. S., & Dollard, S. C. (2008). Congenital cytomegalovirus (CMV) infection as a cause of permanent bilateral hearing loss: a quantitative assessment. Journal of Clinical Virology, 41, 57–62.
Li, Z. J., & Hu, X. Q. (2004). Clinical observation of brainstem auditory evoked potential in infants with cytomegalovirus infections. Chinese Journal of Rehabilitation, 19, 224–225.
Han, L. P., Jiang, Y., & Tao, Y. (2006). Clinical characteristics and outcome of cytomegalovirus infection in infants. Journal of Applied Clinical Pediatrics, 21, 410–411.
Cheng, B. H., Wen, L. Z., & Zhang, Y. (2007). Expression of high mobility group box chromosomal protein 1 in inner ear of mice infected by murine cytomegalovirus. The Journal of Practical Medicine, 23, 336–338.
Acknowledgments
We thank the Affiliated Hospital of Xuzhou Medical University, Xuzhou, China for financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Qiao, Y., Meng, L., Wang, J. et al. Effect of Ganciclovir on Murine Cytomegalovirus-induced Hearing Loss in a Mouse Model. Cell Biochem Biophys 61, 407–412 (2011). https://doi.org/10.1007/s12013-011-9202-8
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12013-011-9202-8