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Effect of Zymosan on Antioxidant and Immune Function of S180 Tumor-Bearing Mice

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Abstract

The objective is to study the effect of zymosan on antioxidant and immune function of S180 tumor-bearing mice. Seventy Kunming mice were randomly divided into seven groups: a normal control group (NC), a tumor control group (TC), three dose groups of zymosan (low, medium, high), a cyclophosphamide (Cy) group, and a combination of zymosan and Cy group. The S180 tumor-bearing mice model was established by the inoculation of cancer cell suspension subcutaneously in the mouse’s right anterior limb. At the 19th day, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activity in liver homogenate were analyzed. The reverse transcriptase-polymerase chain reaction was used to determine the mRNA expression levels of IL-2, TNF-α, and TGF-β1. The activity of GSH-Px and SOD in the liver increased with the dose of zymosan, whereas the activity of MDA significantly decreased in the higher-dose groups of zymosan, compared to the TC group (P < 0.01). In the zymosan groups, mRNA expression levels in tissues of S180 tumor-bearing mice were significantly higher for TNF-α and IL-2, but lower for TGFβ1 than in the Cy or TC group (P < 0.01). The high-dose of zymosan markedly showed a depressant effect on S180 tumor, enhanced by the action of Cy that increased mRNA expression levels of TNF-α and IL-2. The mechanism of zymosan on the inhibition of tumor growth may be due to its ability to enhance the antioxidant and immune function in a dose-dependent manner.

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Abbreviations

Cy:

Cyclophosphamide

GSH-Px:

Glutathione peroxidase

H:

High-dose

L:

Low-dose

M:

Medium-dose

MDA:

Malondialdehyde

NC:

Normal control

RT-PCR:

Reverse transcriptase-polymerase chain reaction

SD:

Standard deviation

SOD:

Superoxide dismutase

TC:

Tumor control

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Acknowledgments

The authors thank Dr. L. V. Xiang for assistance in animal modeling. The support of Dr. Yao Lu for manuscript editing is also gratefully acknowledged.

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Authors and Affiliations

  1. Department of Clinical Nutrition, Changhai Hospital, The First Affiliated Hospital of The Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China

    Xiao-li Liu, Ning Lin, Deng Zan, Jing-Jue Yuan & Dong-Lian Cai

  2. Department of Clinical Nutrition, The Military General Hospital of Chengdu PLA, Chengdu, China

    Ning Lin

Authors
  1. Xiao-li Liu
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  2. Ning Lin
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  3. Deng Zan
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  4. Jing-Jue Yuan
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  5. Dong-Lian Cai
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Corresponding author

Correspondence to Dong-Lian Cai.

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Xiao-li Liu and Ning Lin contributed equally to this work.

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Liu, Xl., Lin, N., Zan, D. et al. Effect of Zymosan on Antioxidant and Immune Function of S180 Tumor-Bearing Mice. Cell Biochem Biophys 60, 225–229 (2011). https://doi.org/10.1007/s12013-010-9143-7

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  • DOI: https://doi.org/10.1007/s12013-010-9143-7

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