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Interference Microscopy in Cell Biophysics. 1. Principles and Methodological Aspects of Coherent Phase Microscopy

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Abstract

The purpose of our tandem publications is to review the applications of the coherent phase microscopy to cell biophysics. In this article, we briefly consider the fundamentals and methodological aspects of the coherent phase microscopy (CPM). One of important advantages of this method is a high sensitivity of CPM images to changes in physical–chemical properties of biological samples. The optical path length difference (OPLD), measured with CPM instruments in different domains of an object, serves as the informative optical parameter used for visualization of individual cells or intracellular organelles. Metabolically dependent variations of CPM images reflect changes in the functional state of biological objects. In the next article, we review results of CPM studies of different cells and intracellular energy-transducing organelles.

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Abbreviations

CPM:

Coherent phase microscopy or coherent phase microscope

DPM:

Dynamic phase microscopy

OPLD:

Optical path length difference

h(x,y):

2D distribution of the OPDL values in the image plane (x,y)

h(x):

The OPDL profile along a scan-line

Δh :

The height of the OPDL profile

Δn :

Refractivity

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Acknowledgments

The authors express their deep gratitude to I. Mazalov, D. Ublinsky, and S. Klushin who actively participated in the development of CPM “Airyscan”. We also thank A. Tavrov, G. Kufal, E. Perevedentseva, T. Vyshenskaya. A. Kretushev, and I. Klemjashov for their active participation in experiments on CPM imaging of biological objects. It is our debt of gratitude to say that A. Kretushev has made a basic contribution to the software development and the improvement of the instrument characteristics. We are also grateful to Dr. A.A. Shtil and Prof. L.S. Yagudzinsky for fruitful cooperation. We thank the anonymous referees for critical reading of the manuscript. The study was partly supported by the Russian Foundation for Basic Researches (grants 07-04-00473, 09-04-00978).

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Authors and Affiliations

  1. Moscow State Institute for Radioengineering, Electronics and Automation, prosp. Vernadskogo 78, Moscow, Russia, 119454

    Vladimir P. Tychinsky

  2. Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, Russia, 119992

    Alexander N. Tikhonov

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  1. Vladimir P. Tychinsky
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Correspondence to Vladimir P. Tychinsky or Alexander N. Tikhonov.

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Tychinsky, V.P., Tikhonov, A.N. Interference Microscopy in Cell Biophysics. 1. Principles and Methodological Aspects of Coherent Phase Microscopy. Cell Biochem Biophys 58, 107–116 (2010). https://doi.org/10.1007/s12013-010-9114-z

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