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Plasma Factor in Red Blood Cells Adhesion to Endothelial Cells: Humans and Rats

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Abstract

Erythrocyte adhesion to the vascular endothelium is one of the key determinants of microcirculatory blood flow. Adhesion is a complex process determined by the intricate interaction among red blood cells (RBC), plasma factors, and the vascular endothelium. Rats are commonly used as disease models to investigate the pathophysiology of various hematological disease processes occurring in humans and their response to prospective treatments. The aim of our study was to characterize the adhesion of RBC in adult blood from rat and human subjects, in order to test the validity of rat models for adhesion-related disease processes. We demonstrated that adhesion of RBC from rats (rRBC), to endothelial cells (EC) in plasma-free buffer, is stronger than from human subjects (hRBC). In addition, plasma proteins induced elevation of hRBC (eightfold) but depression of rRBC (threefold) adhesion to EC. It is thus suggested to be aware of the difference in RBC/EC interaction for human and rat subjects, when studying models of blood flow.

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Acknowledgments

This work was supported by grants from the National Blood Foundation (to S. Yedgar and G. Barshtein), the Israel Science Foundation No. 558/03 (to S. Yedgar and G. Barshtein), and the Walter and Greta Stiel Chair for Heart Studies (to S. Yedgar). The authors thank Mrs. O. Fredman for his technical assistance.

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Authors and Affiliations

  1. Surgery Department, New York University Hospital, New York, NY, USA

    Avraham Schlager

  2. Surgery Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

    Gideon Zamir

  3. Department of Biochemistry, The Faculty of Medicine, The Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel

    Gregory Barshtein & Saul Yedgar

  4. Pediatric Surgery Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

    Dan Arbell

Authors
  1. Avraham Schlager
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  2. Gideon Zamir
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  3. Gregory Barshtein
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  4. Saul Yedgar
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  5. Dan Arbell
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Correspondence to Gregory Barshtein.

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Schlager, A., Zamir, G., Barshtein, G. et al. Plasma Factor in Red Blood Cells Adhesion to Endothelial Cells: Humans and Rats. Cell Biochem Biophys 58, 157–161 (2010). https://doi.org/10.1007/s12013-010-9102-3

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