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Effect of Kaempferol Pretreatment on Myocardial Injury in Rats

Cardiovascular Toxicology volume 18, pages 312–328 (2018)Cite this article

Abstract

The present study was undertaken to evaluate the effect of kaempferol in isoprenaline (ISP)-induced myocardial injury in rats. ISP was administered subcutaneously for two subsequent days to induce myocardial injury. Assessment of myocardial injury was done by estimation of hemodynamic functions, myocardial infarcted area, cardiac injury markers, lipid profile, oxidative stress, pro-inflammatory cytokines and histopathology of heart and liver. Rats pretreated with kaempferol showed reduction in the myocardial infarcted area and heart rate. However, no improvement was observed in change in body weight, mean arterial, systolic and diastolic blood pressure. Kaempferol showed significant decrease in serum LDH, CK-MB, troponin-I and lipid profile. However, highest dose of kaempferol did not reduce the serum triglyceride level. Further, antioxidant enzymes, SOD and catalase, were also higher. However, reduced glutathione, serum SGOT and creatinine did not show any improvement. Kaempferol showed reduction in MDA level. Kaempferol at highest dose showed reduction in pro-MMP-2 expression and MMP-9 level. mRNA expression level of TNF-α was not different in kaempferol-pretreated myocardial injured rats with ISP-alone group. Pretreatment with kaempferol at highest dose showed mild mononuclear infiltration and degenerative changes in heart tissue section of myocardial injured rats. Rats pretreated with kaempferol at higher concentration showed normal cordlike arrangement of hepatocytes with moderate swelling of hepatocytes (vacuolar degeneration) around the central vein. Study suggests that kaempferol attenuated lipid profile, infarcted area and oxidative stress in ISP-induced myocardial injury in rats.

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Acknowledgements

Authors are thankful to the Director, ICAR-Indian Veterinary Research Institute, Izatnagar, for providing the necessary facilities to complete this work.

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  1. Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    Anamika Vishwakarma, Thakur Uttam Singh, Soya Rungsung, Tarun Kumar, Arunvikram Kandasamy, Subhashree Parida, Madhu Cholenahalli Lingaraju & Dinesh Kumar

  2. Division of Animal Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    Ajay Kumar

  3. Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    Asok Kumar

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  1. Anamika Vishwakarma
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Vishwakarma, A., Singh, T.U., Rungsung, S. et al. Effect of Kaempferol Pretreatment on Myocardial Injury in Rats. Cardiovasc Toxicol 18, 312–328 (2018). https://doi.org/10.1007/s12012-018-9443-5

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Keywords

  • Myocardial injury
  • Isoprenaline
  • Kaempferol
  • Rats