Cardiovascular Toxicology

, Volume 17, Issue 2, pp 185–189 | Cite as

The Correlation Between 9p21 Chromosome rs4977574 Polymorphism Genotypes and the Development of Coronary Artery Heart Disease

  • Oushan Tang
  • Jin Lv
  • Yinhong Cheng
  • Fengming Qin


Our aim is to investigate the correlation between 9p21 chromosome rs4977574 polymorphism genotypes and the development of coronary artery heart disease (CHD). Two hundred and eighty-nine patients with angiography-confirmed CHD were recruited as the CHD group, while 338 subjects without CHD symptoms were enrolled as the control group. For all participating subjects, the genotypes of rs4977574 polymorphism were examined by the real-time PCR analysis. Analyses acquired from single-locus technique showed that genotype distribution of rs4977574 polymorphism was significantly different (p = 0.041) between CHD group and the control group. Logistic regression analysis demonstrated that rs4977574 polymorphism in a dominant mode significantly increased (p = 0.038) the risk of CHD, where odds ratio (OR) was 0.71 and the 95 % confidence interval (CI) 0.58–0.97 was applied. 9p21 chromosome rs4977574 polymorphism genotypes are associated with the incidence and development of CHD. The presence of C allele may reduce the risk of CHD.


Coronary artery heart disease Gene polymorphism Glycosylated hemoglobin 



Coronary heart disease


Odds ratio


Confidence interval


Low-density lipoprotein cholesterol


High-density lipoprotein


Homozygous genotype for the T allele


T/C heterozygous genotype


Homozygous for the C allele


Single nucleotide polymorphism


Author Contributions

O.T., J.L., and Y.C. carried out the study and statistical analysis and drafted the manuscript. OT and FQ participated in the experiment design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest



  1. 1.
    Sathyamurthy, I., & Jayanthi, K. (2014). Dual antiplatelet therapy in acute coronary syndromes and coronary artery interventions. Journal of the Association of Physicians of India, 62, 596–601.PubMedGoogle Scholar
  2. 2.
    Ducrocq, G., & Steg, P. G. (2015). Treating coronary artery disease in patients with a history of cerebrovascular disease. Archives of Cardiovascular Diseases, 108(11), 606–611.CrossRefPubMedGoogle Scholar
  3. 3.
    Neelankavil, J., Rau, C. D., & Wang, Y. (2015). The genetic basis of coronary artery disease and atrial fibrillation: A search for disease mechanisms and therapeutic targets. Journal of Cardiothoracic and Vascular Anesthesia, 29, 1328–1332.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Roberts, R. (2014). Genetics of coronary artery disease. Circulation Research, 114, 1890–1903.CrossRefPubMedGoogle Scholar
  5. 5.
    Munir, M. S., Wang, Z., Alahdab, F., et al. (2014). The association of 9p21-3 locus with coronary atherosclerosis: A systematic review and meta-analysis. BMC Medical Genetics, 15, 66.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Rivera, N. V., Carreras-Torres, R., Roncarati, R., et al. (2013). Assessment of the 9p21.3 locus in severity of coronary artery disease in the presence and absence of type 2 diabetes. BMC Medical Genetics, 14, 11.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Assimes, T. L., Knowles, J. W., Basu, A., et al. (2008). Susceptibility locus for clinical and subclinical coronary artery disease at chromosome 9p21 in the multi-ethnic ADVANCE study. Human Molecular Genetics, 17, 2320–2328.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Lee, I. T., Goodarzi, M. O., Lee, W. J., et al. (2014). The chromosome 9p21 variant not predicting long-term cardiovascular mortality in chinese with established coronary artery disease: An eleven-year follow-up study. Biomed Research International, 2014, 626907. doi: 10.1155/2014/626907.PubMedPubMedCentralGoogle Scholar
  9. 9.
    Huang, Yi, Ye, Huadan, Hong, Qingxiao, et al. (2014). Association of CDKN2BAS polymorphism rs4977574 with coronary heart disease: A case-control study and a meta-analysis. International Journal of Molecular Sciences, 15(10), 17478–17492.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Saxena, R., Voight, B. F., Lyssenko, V., et al. (2007). Genome-wide association analysis identifies loci for type 2 diabetes and triglyceride levels. Science, 316, 1331–1336.CrossRefPubMedGoogle Scholar
  11. 11.
    Scott, L. J., Mohlke, K. L., Bonnycastle, L. L., et al. (2007). A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants. Science, 316, 1341–1345.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Doria, A., Wojcik, J., Xu, R., et al. (2008). Interaction between poor glycemic control and 9p21 locus on risk of coronary artery disease in type 2 diabetes. JAMA, 300, 2389–2397.CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Pipe, A. L., Papadakis, S., & Reid, R. D. (2010). The role of smoking cessation in the prevention of coronary artery disease. Current Atherosclerosis Reports, 12, 145–150.CrossRefPubMedGoogle Scholar
  14. 14.
    Pechlivanis, S., Muhleisen, T. W., Mohlenkamp, S., et al. (2013). Risk loci for coronary artery calcification replicated at 9p21 and 6q24 in the Heinz Nixdorf recall study. BMC Medical Genetics, 14, 23.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Preuss, M., Konig, I. R., Thompson, J. R., et al. (2010). Design of the coronary artery disease genome-wide replication and meta-analysis (CARDIoGRAM) study: A genome-wide association meta-analysis involving more than 22000 cases and 60000 controls. Circulation Cardiovascular Genetics, 3, 475–483.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Samani, N. J., Erdmann, J., Hall, A. S., et al. (2007). Genomewide association analysis of coronary artery disease. New England Journal of Medicine, 357, 443–453.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Oushan Tang
    • 1
  • Jin Lv
    • 1
  • Yinhong Cheng
    • 1
  • Fengming Qin
    • 1
  1. 1.Department of CardiologySecond Hospital of ShaoxingShaoxingChina

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