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Cardioprotective Effects of Total Flavonoids Extracted from Xinjiang Sprig Rosa rugosa against Acute Ischemia/Reperfusion-Induced Myocardial Injury in Isolated Rat Heart

Abstract

This study evaluated the antioxidative and cardioprotective effects of total flavonoids extracted from Xinjiang sprig Rosa rugosa on ischemia/reperfusion (I/R) injury using an isolated Langendorff rat heart model. The possible mechanism of Xinjiang sprig rose total flavonoid (XSRTF) against I/R injury was also studied. XSRTF (5, 10, and 20 µg/mL) dissolved in Krebs–Henseleit buffer was administered to isolated rat heart. The XSRTF showed remarkable scavenging effects against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide anion radicals in vitro. XSRTF pretreatment improved the heart rate, increased LVDP, and decreased CK and LDH levels in coronary flow. This pretreatment also increased SOD activity and GSH/GSSG ratio but decreased MDA, TNF-α, and CRP levels and IL-8 and IL-6 activities. The infarct size and cell apoptosis in the hearts from the XSRTF-treated group were lower than those in the hearts from the I/R group. Therefore, the cardioprotective effects of XSRTF may be attributed to its antioxidant, antiapoptotic, and anti-inflammatory activities.

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Acknowledgments

This study was supported by the Xinjiang characteristics of natural medicine modernization and development of key technology research (2012BAI30B01) to Wen Chen, the Xinjiang production and Construction Corps funds for Distinguished Young Scientists (2011CD006) to Qiusheng Zheng.

Conflict of interests

The authors declare that they have no financial conflict of interests.

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Correspondence to Qiusheng Zheng or Wen Chen.

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Xuejiao Hou and Jichun Han have contributed equally to this article.

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Hou, X., Han, J., Yuan, C. et al. Cardioprotective Effects of Total Flavonoids Extracted from Xinjiang Sprig Rosa rugosa against Acute Ischemia/Reperfusion-Induced Myocardial Injury in Isolated Rat Heart. Cardiovasc Toxicol 16, 54–66 (2016). https://doi.org/10.1007/s12012-015-9308-0

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  • DOI: https://doi.org/10.1007/s12012-015-9308-0

Keywords

  • Xinjiang sprig Rosa rugosa
  • Total flavonoid
  • Antioxidant
  • Antiapoptotic
  • Anti-inflammatory
  • Cardioprotective activity
  • Ischemia/reperfusion