Skip to main content

Infrasound Exposure Induces Apoptosis of Rat Cardiac Myocytes by Regulating the Expression of Apoptosis-Related Proteins

Cardiovascular Toxicology volume 11, Article number: 341 (2011) Cite this article

Abstract

It has been reported that exposure to infrasound causes cardiac dysfunction. Allowing for the key role of apoptosis in the pathogenesis of cardiovascular diseases, the objective of this study was to investigate the apoptotic effects of infrasound. Cardiac myocytes cultured from neonatal rats were exposed to infrasound of 5 Hz at 130 dB. The apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Also, the expression levels of a series of apoptosis-related proteins were detected. As a result, infrasound induced apoptosis of cultured rat cardiac myocytes in a time-dependant manner. The expression of proapoptotic proteins such as Bax, caspase-3, caspase-8, caspase-9, and FAS was significantly up-regulated, with concomitant down-regulated expression of antiapoptotic proteins such as Bcl-x, and the inhibitory apoptosis proteins family proteins including XIAP, cIAP-1, and cIAP-2. The expression of poly (ADP-ribose) polymerase and β-catenin, which are the substrate proteins of caspase-3, was significantly decreased. In conclusion, infrasound is an apoptotic inducer of cardiac myocytes.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

39,95 €

Price includes VAT (Finland)

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Leventhall, G. (2007). What is infrasound? Progress in Biophysics and Molecular Biology, 93(1–3), 130–137.

    PubMed  Article  Google Scholar 

  2. Liu, Z. H., Chen, J. Z., Tang, Y., Chen, D., Ding, G. R., Liu, J., et al. (2004). Effects of infrasound on changes of intracellular calcium ion concentration and on expression of RyRs in hippocampus of rat brain. Journal of Low Frequency Noise, Vibration and Active Control, 23, 159–165.

    Google Scholar 

  3. Liu, J., Lin, T., Yan, X., Jiang, W., Shi, M., Ye, R., et al. (2010). Effects of infrasound on cell proliferation in the dentate gyrus of adult rats. Neuroreport, 21(8), 585–589.

    PubMed  Article  Google Scholar 

  4. Dang, W. M., Wang, S., Tian, S. X., Chen, B., Sun, F., Li, W., et al. (2007). Effects of infrasound on activities of 3beta hydroxysteroid dehydrogenase and acid phosphatase of polygonal cells in adrenal cortex zona fasciculate in mice. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi, 25(2), 91–95.

    PubMed  CAS  Google Scholar 

  5. Zhuang, Z., Pei, Z., & Chen, J. (2007). Infrasound-induced changes on sexual behavior in male rats and some underlying mechanisms. Environmental Toxicology and Pharmacology, 23, 111–114.

    PubMed  Article  CAS  Google Scholar 

  6. Gordeladze, A. S., Glinchikov, V. V., & Usenko, V. R. (1986). Experimental myocardial ischemia caused by infrasound. Gigiena truda i professional’nye zabolevaniia, 6, 30–33.

    PubMed  Google Scholar 

  7. Alekseev, S. V., Glinchikov, V. V., & Usenko, V. R. (1983). Myocardial ischemia in rats exposed to infrasound. Gigiena truda i professional’nye zabolevaniia, 8, 34–38.

    PubMed  Google Scholar 

  8. Pei, Z., Sang, H., Li, R., Xiao, P., He, J., Zhuang, Z., et al. (2007). Infrasound-induced hemodynamics, ultrastructure, and molecular changes in the rat myocardium. Environmental Toxicology, 22(2), 169–175.

    PubMed  Article  CAS  Google Scholar 

  9. Pei, Z., Zhuang, Z., Xiao, P., Chen, J., Sang, H., Ren, J., et al. (2009). Influence of infrasound exposure on the whole L-type calcium currents in rat ventricular myocytes. Cardiovascular Toxicology, 9(2), 70–77.

    PubMed  Article  CAS  Google Scholar 

  10. Lee, Y., & Gustafsson, A. B. (2009). Role of apoptosis in cardiovascular disease. Apoptosis, 14(4), 536–548.

    PubMed  Article  Google Scholar 

  11. Lü, S. Y., Zhu, M. Z., Wang, D. S., Yu, J., Guo, H. T., Hu, Y. Z., et al. (2004). Down-regulation of C-type natriuretic peptide receptor by vasonatrin peptide in cardiac myocytes and fibroblasts. Acta Pharmacologica Sinica, 25(4), 424–430.

    PubMed  Google Scholar 

  12. Tuo, Q. H., Liang, L., Zhu, B. Y., Cao, X., & Liao, D. F. (2008). Effect of Daxx on cholesterol accumulation in hepatic cells. World Journal of Gastroenterology, 14(3), 435–440.

    PubMed  Article  CAS  Google Scholar 

  13. Yu, J., Bulk, E., Ji, P., Hascher, A., Tang, M., Metzger, R., et al. (2010). The EPHB6 receptor tyrosine kinase is a metastasis suppressor that is frequently silenced by promoter DNA hypermethylation in non-small cell lung cancer. Clinical Cancer Research, 16(8), 2275–2283.

    PubMed  Article  CAS  Google Scholar 

  14. Tiberio, R., Marconi, A., Fila, C., Fumelli, C., Pignatti, M., Krajewski, S., et al. (2002). Keratinocytes enriched for stem cells are protected from anoikis via an integrin signaling pathway in a Bcl-2 dependent manner. FEBS letters, 524(1–3), 139–144.

    PubMed  Article  CAS  Google Scholar 

  15. Alenzi, F. Q., Lotfy, M., & Wyse, R. (2010). Swords of cell death: Caspase activation and regulation. Asian Pacific Journal of Cancer Prevention, 11(2), 271–280.

    PubMed  Google Scholar 

  16. Fukuda, K. (1999). Apoptosis-associated cleavage of beta-catenin in human colon cancer and rat hepatoma cells. International Journal of Biochemistry and Cell Biology, 31(3–4), 519–529.

    PubMed  Article  CAS  Google Scholar 

  17. Chaitanya, G. V., Steven, A. J., & Babu, P. P. (2010). PARP-1 cleavage fragments: Signatures of cell-death proteases in neurodegeneration. Cell Communication and Signaling, 8, 31.

    PubMed  Article  CAS  Google Scholar 

  18. Gyrd-Hansen, M., & Meier, P. (2010). IAPs: From caspase inhibitors to modulators of NF-kappaB, inflammation and cancer. Nature Reviews Cancer, 10(8), 561–574.

    PubMed  Article  CAS  Google Scholar 

  19. Strasser, A., Jost, P. J., & Nagata, S. (2009). The many roles of FAS receptor signaling in the immune system. Immunity, 30(2), 180–192.

    PubMed  Article  CAS  Google Scholar 

  20. Bao, Y., Fan, J. Z., Li, K., Li, C., & Yang, J. F. (2008). Effects of infrasound therapy on proliferation, apoptosis and ultrastructure of human B lymphoma Raji cells. Nan Fang Yi Ke Da Xue Xue Bao, 28(6), 1093–1095.

    PubMed  Google Scholar 

  21. Liu, Z. H., Chen, J. Z., Ye, L., Liu, J., Qiu, J. Y., Xu, J., et al. (2010). Effects of infrasound at 8 Hz 90 dB/130 dB on NMDAR1 expression and changes in intracellular calcium ion concentration in the hippocampus of rats. Molecular medicine reports, 3(6), 917–921.

    PubMed  Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30870579, 30870902, 30801385), Special grade of the financial support from China Postdoctoral Science Foundation (No. 200801266), New Medicine Research Fund (No. 2009ZX09103-671), and Natural Science Fund of Shaan’xi Province (No. 2010JM4050).

Author information

Authors and Affiliations

  1. Department of Cardiology, Third Hospital, Nanchang, 330009, Jiangxi, China

    Zhao-Hui Pei

  2. Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi’an, China

    Bao-Ying Chen

  3. Center of Teaching Experiment, School of Basic Medical Science, Fourth Military Medical University, 169 Changle West Road, Xi’an, Shaanxi, China

    Ru Tie, Hai-Feng Zhang, Ping Qu & Jun Yu

  4. Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, China

    Ge Zhao

  5. Department of Pharmacology, Fourth Military Medical University, Xi’an, China

    Xiao-Xing Zhu

  6. Department of Physiology, Fourth Military Medical University, Xi’an, China

    Miao-Zhang Zhu

Authors
  1. Zhao-Hui Pei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bao-Ying Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ru Tie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hai-Feng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ge Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ping Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiao-Xing Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Miao-Zhang Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jun Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Miao-Zhang Zhu or Jun Yu.

Additional information

Zhao-Hui Pei, Bao-Ying Chen, Ru Tie and Hai-Feng Zhang contributed equally to this work.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Pei, ZH., Chen, BY., Tie, R. et al. Infrasound Exposure Induces Apoptosis of Rat Cardiac Myocytes by Regulating the Expression of Apoptosis-Related Proteins. Cardiovasc Toxicol 11, 341 (2011). https://doi.org/10.1007/s12012-011-9126-y

Download citation

  • Published:

  • DOI: https://doi.org/10.1007/s12012-011-9126-y

Keywords

  • Infrasound
  • Apoptosis
  • Cardiac myocytes
  • Bcl-x
  • IAPs
  • Caspase