Pomegranate (Punica granatum L.) Juice Supplementation Attenuates Isoproterenol-Induced Cardiac Necrosis in Rats


The aim of the present study was to evaluate the efficacy of pre-supplementation with pomegranate (Punica granatum L.) juice (PJ) on heart weight, infarct size, plasma marker enzymes of cardiac damage, lipid peroxidation, endogenous enzymatic and non-enzymatic antioxidants, cardiac ATPases and histopathology of isoproterenol (IP)-induced cardiac necrosis (CN) in rats. Rats treated with IP (85 mg/kg, s.c.) for 2 days at an interval of 24 h caused significant (P < 0.05) infarct in myocardium and increase in heart weight, lipid peroxidation (LPO), activity levels of Ca+2 ATPase and plasma marker enzymes, while there was significant (P < 0.05) decrease in endogenous enzymatic and non-enzymatic antioxidants and Na+-K+ and Mg+2ATPases. Pre-supplementation with PJ for 30 consecutive days and treated with IP on days 29th and 30th showed significantly (P < 0.05) lesser increase in heart weight, infarct size, plasma marker enzymes, lipid peroxidation, Ca+2 ATPase and a significant protective effect in endogenous enzymatic and non-enzymatic antioxidants, Na+-K+ and Mg+2 ATPases compared to IP alone treated group. Present study provides first scientific report on protective effect of supplementation of Pomegranate juice against IP-induced CN in rats.

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The authors are grateful to University Grants Commission, New Delhi for providing Financial Assistance in the form of JRFSMS scholarship. Thanks are also due to Zydus Research Centre, Ahmedabad, India for providing experimental rats.

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Correspondence to Ranjitsinh V. Devkar.

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Jadeja, R.N., Thounaojam, M.C., Patel, D.K. et al. Pomegranate (Punica granatum L.) Juice Supplementation Attenuates Isoproterenol-Induced Cardiac Necrosis in Rats. Cardiovasc Toxicol 10, 174–180 (2010). https://doi.org/10.1007/s12012-010-9076-9

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  • Punica granatum L
  • Isoproterenol
  • Cardiac necrosis
  • Free radicals
  • Oxidative stress