Abstract
In this study, we investigated the protective effect of selenium (Se)-enriched peptide isolated from Cardamine violifolia (SPE) against ethanol-induced liver injury. Cell proliferation assays show that different concentrations of SPE protect human embryonic liver L-02 cells against ethanol-induced injury in a dose-dependent manner. Treatment with 12 μmol/L Se increases the cell survival rate (82.44%) and reduces the release of alanine aminotransferase, aspartate transaminase, lactate dehydrogenase, and apoptosis rate. SPE treatment with 12 μmol/L Se effectively reduces the concentration of intracellular reactive oxygen species and increases the contents of intracellular superoxide dismutase (51.64 U/mg), catalase (4.41 U/mg), glutathione peroxidase (1205.28 nmol/g), and glutathione (66.67 μmol/g), thereby inhibiting the effect of ethanol-induced oxidative damage. The results of the transcriptomic analysis show that the glutathione metabolism and apoptotic pathway play significant roles in the protection of L-02 hepatocytes by SPE. Real-time qPCR analysis shows that SPE increases the mRNA expression of GPX1 and NGFR. The results of this study highlight the protective effects of SPE against ethanol-induced liver injury.
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Funding
This work was supported financially by the National Natural Science Foundation of China (No. 32172197, 32272314), the Innovative Project of the State Key Laboratory of Food Science and Technology (SKLF-ZZB-202307), and the program of “Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,” China.
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L. R. and W. S. wrote the main manuscript text. All authors reviewed the manuscript.
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Ling, R., Du, C., Li, Y. et al. Protective Effect of Selenium-enriched Peptide from Cardamine violifolia on Ethanol-induced L-02 Hepatocyte Injury. Biol Trace Elem Res (2024). https://doi.org/10.1007/s12011-024-04159-8
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DOI: https://doi.org/10.1007/s12011-024-04159-8